Impaired Expression of Cytoplasmic Actins Leads to Chromosomal Instability of MDA-MB-231 Basal-Like Mammary Gland Cancer Cell Line
We have shown previously that two cytoplasmic actin isoforms play different roles in neoplastic cell transformation. Namely, β-cytoplasmic actin acts as a tumor suppressor, whereas γ-cytoplasmic actin enhances malignant features of tumor cells. The distinct participation of each cytoplasmic actin in...
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MDPI AG
2021-04-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/26/8/2151 |
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| author | Vera Dugina Galina Shagieva Mariya Novikova Svetlana Lavrushkina Olga Sokova Igor Kireev Pavel Kopnin |
| author_facet | Vera Dugina Galina Shagieva Mariya Novikova Svetlana Lavrushkina Olga Sokova Igor Kireev Pavel Kopnin |
| author_sort | Vera Dugina |
| collection | DOAJ |
| description | We have shown previously that two cytoplasmic actin isoforms play different roles in neoplastic cell transformation. Namely, β-cytoplasmic actin acts as a tumor suppressor, whereas γ-cytoplasmic actin enhances malignant features of tumor cells. The distinct participation of each cytoplasmic actin in the cell cycle driving was also observed. The goal of this study was to describe the diverse roles of cytoplasmic actins in the progression of chromosomal instability of MDA-MB-231 basal-like human carcinoma cell line. We performed traditional methods of chromosome visualization, as well as 3D-IF microscopy and western blotting for CENP-A detection/quantification, to investigate chromosome morphology. Downregulation of cytoplasmic actin isoforms alters the phenotype and karyotype of MDA-MB-231 breast cancer cells. Moreover, β-actin depletion leads to the progression of chromosomal instability with endoreduplication and aneuploidy increase. On the contrary, γ-actin downregulation results not only in reduced percentage of mitotic carcinoma cells, but leads to chromosome stability, reduced polyploidy, and aneuploidy. |
| first_indexed | 2024-03-10T12:30:16Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T12:30:16Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
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| series | Molecules |
| spelling | doaj.art-c5060d069b3e4e6bb90bab0bb296b81a2023-11-21T14:43:46ZengMDPI AGMolecules1420-30492021-04-01268215110.3390/molecules26082151Impaired Expression of Cytoplasmic Actins Leads to Chromosomal Instability of MDA-MB-231 Basal-Like Mammary Gland Cancer Cell LineVera Dugina0Galina Shagieva1Mariya Novikova2Svetlana Lavrushkina3Olga Sokova4Igor Kireev5Pavel Kopnin6Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 119992 Moscow, RussiaBelozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 119992 Moscow, RussiaN.N. Blokhin National Medical Research Center of Oncology, 115478 Moscow, RussiaBelozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 119992 Moscow, RussiaN.N. Blokhin National Medical Research Center of Oncology, 115478 Moscow, RussiaBelozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 119992 Moscow, RussiaN.N. Blokhin National Medical Research Center of Oncology, 115478 Moscow, RussiaWe have shown previously that two cytoplasmic actin isoforms play different roles in neoplastic cell transformation. Namely, β-cytoplasmic actin acts as a tumor suppressor, whereas γ-cytoplasmic actin enhances malignant features of tumor cells. The distinct participation of each cytoplasmic actin in the cell cycle driving was also observed. The goal of this study was to describe the diverse roles of cytoplasmic actins in the progression of chromosomal instability of MDA-MB-231 basal-like human carcinoma cell line. We performed traditional methods of chromosome visualization, as well as 3D-IF microscopy and western blotting for CENP-A detection/quantification, to investigate chromosome morphology. Downregulation of cytoplasmic actin isoforms alters the phenotype and karyotype of MDA-MB-231 breast cancer cells. Moreover, β-actin depletion leads to the progression of chromosomal instability with endoreduplication and aneuploidy increase. On the contrary, γ-actin downregulation results not only in reduced percentage of mitotic carcinoma cells, but leads to chromosome stability, reduced polyploidy, and aneuploidy.https://www.mdpi.com/1420-3049/26/8/2151mammary gland canceractin isoformschromosomal instability (CIN) |
| spellingShingle | Vera Dugina Galina Shagieva Mariya Novikova Svetlana Lavrushkina Olga Sokova Igor Kireev Pavel Kopnin Impaired Expression of Cytoplasmic Actins Leads to Chromosomal Instability of MDA-MB-231 Basal-Like Mammary Gland Cancer Cell Line Molecules mammary gland cancer actin isoforms chromosomal instability (CIN) |
| title | Impaired Expression of Cytoplasmic Actins Leads to Chromosomal Instability of MDA-MB-231 Basal-Like Mammary Gland Cancer Cell Line |
| title_full | Impaired Expression of Cytoplasmic Actins Leads to Chromosomal Instability of MDA-MB-231 Basal-Like Mammary Gland Cancer Cell Line |
| title_fullStr | Impaired Expression of Cytoplasmic Actins Leads to Chromosomal Instability of MDA-MB-231 Basal-Like Mammary Gland Cancer Cell Line |
| title_full_unstemmed | Impaired Expression of Cytoplasmic Actins Leads to Chromosomal Instability of MDA-MB-231 Basal-Like Mammary Gland Cancer Cell Line |
| title_short | Impaired Expression of Cytoplasmic Actins Leads to Chromosomal Instability of MDA-MB-231 Basal-Like Mammary Gland Cancer Cell Line |
| title_sort | impaired expression of cytoplasmic actins leads to chromosomal instability of mda mb 231 basal like mammary gland cancer cell line |
| topic | mammary gland cancer actin isoforms chromosomal instability (CIN) |
| url | https://www.mdpi.com/1420-3049/26/8/2151 |
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