Antimicrobial activity of essential oils extracted from clove and lemongrass against pathogenic bacteria isolated from bovine, swine and poultry feces
The objective was to verify the antibacterial activity of lemongrass and clove oils against Escherichia coli isolated from poultry feces, Staphylococcus aureus isolated from swine and poultry feces and Salmonella sp. isolated from swine and bovine feces. The antimicrobial activity was evaluated by t...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Universidade Estadual de Londrina
2019-07-01
|
| Series: | Semina: Ciências Agrárias |
| Subjects: | |
| Online Access: | https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/33753 |
| _version_ | 1828055366783467520 |
|---|---|
| author | Stephanie Pedrosa de Oliveira Gabriel Santos Persiquini Cunha João Paulo Bicalho Prates Francine Souza Alves Fonseca Keicy Sandy Silvestre de Souza Alcinei Místico Azevedo Alessandra Rejane Ericsson de Oliveira Xavier Eliane Macedo Sobrinho Santos Hércules Otacílio Santos Anna Christina de Almeida |
| author_facet | Stephanie Pedrosa de Oliveira Gabriel Santos Persiquini Cunha João Paulo Bicalho Prates Francine Souza Alves Fonseca Keicy Sandy Silvestre de Souza Alcinei Místico Azevedo Alessandra Rejane Ericsson de Oliveira Xavier Eliane Macedo Sobrinho Santos Hércules Otacílio Santos Anna Christina de Almeida |
| author_sort | Stephanie Pedrosa de Oliveira |
| collection | DOAJ |
| description | The objective was to verify the antibacterial activity of lemongrass and clove oils against Escherichia coli isolated from poultry feces, Staphylococcus aureus isolated from swine and poultry feces and Salmonella sp. isolated from swine and bovine feces. The antimicrobial activity was evaluated by the disc diffusion test using different concentrations of the oils: 160, 80, 40, 20 and 10 ?l ml -1. The multi-resistance of strains relative to that of conventional antimicrobials was also evaluated by the disk diffusion technique, using Multiple Antibiotic Resistance (MAR) index. The oils were characterized based on the results of chromatographic analyses, of which, analysis of lemongrass has been previously published (AZEVEDO et al., 2016). Lemongrass and clove essential oils presented citral and eugenol as the major compound, respectively. The concentrations of the essential oils had a significant effect on the extent of the growth inhibition halo and the coefficient of determination (r²) was higher than 0.80. Clove essential oil generated the largest zone of inhibition when tested against Escherichia coli and S. aureus from poultry feces and Salmonella sp. from the feces of swine, while lemongrass essential oil presented better results against S. aureus isolated from swine feces and Salmonella sp. from bovine feces. S. aureus and Salmonella sp. were multi-resistant to the antimicrobials tested. It is concluded that the essential oils tested have antimicrobial activity against bacteria isolated from bovine, swine, and poultry feces and that this activity is proportional to the concentration of oils and the microorganisms studied. |
| first_indexed | 2024-04-10T20:37:33Z |
| format | Article |
| id | doaj.art-c509b6f7b26c494284d96d8452163a0d |
| institution | Directory Open Access Journal |
| issn | 1676-546X 1679-0359 |
| language | English |
| last_indexed | 2024-04-10T20:37:33Z |
| publishDate | 2019-07-01 |
| publisher | Universidade Estadual de Londrina |
| record_format | Article |
| series | Semina: Ciências Agrárias |
| spelling | doaj.art-c509b6f7b26c494284d96d8452163a0d2023-01-24T20:31:15ZengUniversidade Estadual de LondrinaSemina: Ciências Agrárias1676-546X1679-03592019-07-0140510.5433/1679-0359.2019v40n5p1937Antimicrobial activity of essential oils extracted from clove and lemongrass against pathogenic bacteria isolated from bovine, swine and poultry fecesStephanie Pedrosa de Oliveira0Gabriel Santos Persiquini Cunha1João Paulo Bicalho Prates2Francine Souza Alves Fonseca3Keicy Sandy Silvestre de Souza4Alcinei Místico Azevedo5Alessandra Rejane Ericsson de Oliveira Xavier6Eliane Macedo Sobrinho Santos7Hércules Otacílio Santos8Anna Christina de Almeida9Universidade Federal de Minas GeraisUniversidade Federal de Minas GeraisUniversidade Federal de Minas GeraisUniversidade Federal de Minas GeraisUniversidade Federal de Minas GeraisUniversidade Federal de Minas GeraisUniversidade Estadual de Montes ClarosInstituto Federal do Norte de Minas GeraisInstituto Federal do Norte de Minas GeraisUniversidade Federal de Minas GeraisThe objective was to verify the antibacterial activity of lemongrass and clove oils against Escherichia coli isolated from poultry feces, Staphylococcus aureus isolated from swine and poultry feces and Salmonella sp. isolated from swine and bovine feces. The antimicrobial activity was evaluated by the disc diffusion test using different concentrations of the oils: 160, 80, 40, 20 and 10 ?l ml -1. The multi-resistance of strains relative to that of conventional antimicrobials was also evaluated by the disk diffusion technique, using Multiple Antibiotic Resistance (MAR) index. The oils were characterized based on the results of chromatographic analyses, of which, analysis of lemongrass has been previously published (AZEVEDO et al., 2016). Lemongrass and clove essential oils presented citral and eugenol as the major compound, respectively. The concentrations of the essential oils had a significant effect on the extent of the growth inhibition halo and the coefficient of determination (r²) was higher than 0.80. Clove essential oil generated the largest zone of inhibition when tested against Escherichia coli and S. aureus from poultry feces and Salmonella sp. from the feces of swine, while lemongrass essential oil presented better results against S. aureus isolated from swine feces and Salmonella sp. from bovine feces. S. aureus and Salmonella sp. were multi-resistant to the antimicrobials tested. It is concluded that the essential oils tested have antimicrobial activity against bacteria isolated from bovine, swine, and poultry feces and that this activity is proportional to the concentration of oils and the microorganisms studied.https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/33753AntimicrobialCloveCymbopogon flexuosusLemongrassSyzygium aromaticum. |
| spellingShingle | Stephanie Pedrosa de Oliveira Gabriel Santos Persiquini Cunha João Paulo Bicalho Prates Francine Souza Alves Fonseca Keicy Sandy Silvestre de Souza Alcinei Místico Azevedo Alessandra Rejane Ericsson de Oliveira Xavier Eliane Macedo Sobrinho Santos Hércules Otacílio Santos Anna Christina de Almeida Antimicrobial activity of essential oils extracted from clove and lemongrass against pathogenic bacteria isolated from bovine, swine and poultry feces Semina: Ciências Agrárias Antimicrobial Clove Cymbopogon flexuosus Lemongrass Syzygium aromaticum. |
| title | Antimicrobial activity of essential oils extracted from clove and lemongrass against pathogenic bacteria isolated from bovine, swine and poultry feces |
| title_full | Antimicrobial activity of essential oils extracted from clove and lemongrass against pathogenic bacteria isolated from bovine, swine and poultry feces |
| title_fullStr | Antimicrobial activity of essential oils extracted from clove and lemongrass against pathogenic bacteria isolated from bovine, swine and poultry feces |
| title_full_unstemmed | Antimicrobial activity of essential oils extracted from clove and lemongrass against pathogenic bacteria isolated from bovine, swine and poultry feces |
| title_short | Antimicrobial activity of essential oils extracted from clove and lemongrass against pathogenic bacteria isolated from bovine, swine and poultry feces |
| title_sort | antimicrobial activity of essential oils extracted from clove and lemongrass against pathogenic bacteria isolated from bovine swine and poultry feces |
| topic | Antimicrobial Clove Cymbopogon flexuosus Lemongrass Syzygium aromaticum. |
| url | https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/33753 |
| work_keys_str_mv | AT stephaniepedrosadeoliveira antimicrobialactivityofessentialoilsextractedfromcloveandlemongrassagainstpathogenicbacteriaisolatedfrombovineswineandpoultryfeces AT gabrielsantospersiquinicunha antimicrobialactivityofessentialoilsextractedfromcloveandlemongrassagainstpathogenicbacteriaisolatedfrombovineswineandpoultryfeces AT joaopaulobicalhoprates antimicrobialactivityofessentialoilsextractedfromcloveandlemongrassagainstpathogenicbacteriaisolatedfrombovineswineandpoultryfeces AT francinesouzaalvesfonseca antimicrobialactivityofessentialoilsextractedfromcloveandlemongrassagainstpathogenicbacteriaisolatedfrombovineswineandpoultryfeces AT keicysandysilvestredesouza antimicrobialactivityofessentialoilsextractedfromcloveandlemongrassagainstpathogenicbacteriaisolatedfrombovineswineandpoultryfeces AT alcineimisticoazevedo antimicrobialactivityofessentialoilsextractedfromcloveandlemongrassagainstpathogenicbacteriaisolatedfrombovineswineandpoultryfeces AT alessandrarejaneericssondeoliveiraxavier antimicrobialactivityofessentialoilsextractedfromcloveandlemongrassagainstpathogenicbacteriaisolatedfrombovineswineandpoultryfeces AT elianemacedosobrinhosantos antimicrobialactivityofessentialoilsextractedfromcloveandlemongrassagainstpathogenicbacteriaisolatedfrombovineswineandpoultryfeces AT herculesotaciliosantos antimicrobialactivityofessentialoilsextractedfromcloveandlemongrassagainstpathogenicbacteriaisolatedfrombovineswineandpoultryfeces AT annachristinadealmeida antimicrobialactivityofessentialoilsextractedfromcloveandlemongrassagainstpathogenicbacteriaisolatedfrombovineswineandpoultryfeces |