Fabric phase sorptive extraction-gas chromatography-mass spectrometry for the determination of favipiravir in biological and forensic samples
Favipiravir, a pyrazine analog, is proposed as providential antiviral agent against the COVID-19 infection during 2020 pandemic emergency. For the first time, a fabric phase sorptive extraction (FPSE) combined with gas chromatography-mass spectrometry (GC-MS) has been developed and applied for the d...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-05-01
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| Series: | Advances in Sample Preparation |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2772582023000086 |
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| author | Rajeev Jain Bharti Jain Abuzar Kabir Atul Bajaj Ratnasekhar Ch Shweta Sharma |
| author_facet | Rajeev Jain Bharti Jain Abuzar Kabir Atul Bajaj Ratnasekhar Ch Shweta Sharma |
| author_sort | Rajeev Jain |
| collection | DOAJ |
| description | Favipiravir, a pyrazine analog, is proposed as providential antiviral agent against the COVID-19 infection during 2020 pandemic emergency. For the first time, a fabric phase sorptive extraction (FPSE) combined with gas chromatography-mass spectrometry (GC-MS) has been developed and applied for the determination of favipiravir (FAV) in biological samples (human plasma, blood and urine), pharmaceutical and forensic samples. The method comprises of extraction of FAV by FPSE followed by its derivatization with N, O-bis (trimethylsilyl) trifluoroacetamide (BSTFA) and GC-MS analysis. Design of experiment-based optimization was performed using Placket-Burman Design (PBD) and Central Composite Design (CCD) for the screening of significant factors of FPSE and their optimization, respectively. Among all tested membranes, sol-gel polyethylene glycol (PEG) has offered the best extraction efficiency for FAV. Under optimum conditions, the proposed method was found to be linear in the range of 0.01–10 µg mL−1 by GC-MS. The LODs and LOQs were as low as 0.001-0.0026 µg mL−1 and 0.003-0.0086 µg mL−1, respectively by GC-MS. Intra-day and inter-day precisions were less than 5 and 10%, respectively, showing good method precision. The proposed method has been successfully applied to detect and quantify FAV in human urine, whole blood and plasma samples along with seized forensic samples. In addition, the proposed method has been evaluated for its green character by ComplexGAPI index. |
| first_indexed | 2024-03-13T07:57:27Z |
| format | Article |
| id | doaj.art-c50a9161bd5e42d3a4f35847784271e0 |
| institution | Directory Open Access Journal |
| issn | 2772-5820 |
| language | English |
| last_indexed | 2024-03-13T07:57:27Z |
| publishDate | 2023-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Advances in Sample Preparation |
| spelling | doaj.art-c50a9161bd5e42d3a4f35847784271e02023-06-02T04:24:06ZengElsevierAdvances in Sample Preparation2772-58202023-05-016100058Fabric phase sorptive extraction-gas chromatography-mass spectrometry for the determination of favipiravir in biological and forensic samplesRajeev Jain0Bharti Jain1Abuzar Kabir2Atul Bajaj3Ratnasekhar Ch4Shweta Sharma5Central Forensic Science Laboratory, Dakshin Marg, Sector – 36A, Chandigarh 160036, India; Corresponding authors.Central Forensic Science Laboratory, Dakshin Marg, Sector – 36A, Chandigarh 160036, India; Institute of Forensic Science & Criminology, Panjab University, Chandigarh 160014, IndiaDepartment of Chemistry and Biochemistry, Florida International University, Miami, FL, USA; Corresponding authors.Central Forensic Science Laboratory, Dakshin Marg, Sector – 36A, Chandigarh 160036, IndiaMetabolomics Lab, CSIR—Central Institute of Medicinal & Aromatic Plants (CIMAP), Lucknow 226015, IndiaInstitute of Forensic Science & Criminology, Panjab University, Chandigarh 160014, India; Corresponding authors.Favipiravir, a pyrazine analog, is proposed as providential antiviral agent against the COVID-19 infection during 2020 pandemic emergency. For the first time, a fabric phase sorptive extraction (FPSE) combined with gas chromatography-mass spectrometry (GC-MS) has been developed and applied for the determination of favipiravir (FAV) in biological samples (human plasma, blood and urine), pharmaceutical and forensic samples. The method comprises of extraction of FAV by FPSE followed by its derivatization with N, O-bis (trimethylsilyl) trifluoroacetamide (BSTFA) and GC-MS analysis. Design of experiment-based optimization was performed using Placket-Burman Design (PBD) and Central Composite Design (CCD) for the screening of significant factors of FPSE and their optimization, respectively. Among all tested membranes, sol-gel polyethylene glycol (PEG) has offered the best extraction efficiency for FAV. Under optimum conditions, the proposed method was found to be linear in the range of 0.01–10 µg mL−1 by GC-MS. The LODs and LOQs were as low as 0.001-0.0026 µg mL−1 and 0.003-0.0086 µg mL−1, respectively by GC-MS. Intra-day and inter-day precisions were less than 5 and 10%, respectively, showing good method precision. The proposed method has been successfully applied to detect and quantify FAV in human urine, whole blood and plasma samples along with seized forensic samples. In addition, the proposed method has been evaluated for its green character by ComplexGAPI index.http://www.sciencedirect.com/science/article/pii/S2772582023000086Fabric phase sorptive extractionFavipiravirCOVID-19GC-MSAnti-viral drugsForensic chemistry |
| spellingShingle | Rajeev Jain Bharti Jain Abuzar Kabir Atul Bajaj Ratnasekhar Ch Shweta Sharma Fabric phase sorptive extraction-gas chromatography-mass spectrometry for the determination of favipiravir in biological and forensic samples Advances in Sample Preparation Fabric phase sorptive extraction Favipiravir COVID-19 GC-MS Anti-viral drugs Forensic chemistry |
| title | Fabric phase sorptive extraction-gas chromatography-mass spectrometry for the determination of favipiravir in biological and forensic samples |
| title_full | Fabric phase sorptive extraction-gas chromatography-mass spectrometry for the determination of favipiravir in biological and forensic samples |
| title_fullStr | Fabric phase sorptive extraction-gas chromatography-mass spectrometry for the determination of favipiravir in biological and forensic samples |
| title_full_unstemmed | Fabric phase sorptive extraction-gas chromatography-mass spectrometry for the determination of favipiravir in biological and forensic samples |
| title_short | Fabric phase sorptive extraction-gas chromatography-mass spectrometry for the determination of favipiravir in biological and forensic samples |
| title_sort | fabric phase sorptive extraction gas chromatography mass spectrometry for the determination of favipiravir in biological and forensic samples |
| topic | Fabric phase sorptive extraction Favipiravir COVID-19 GC-MS Anti-viral drugs Forensic chemistry |
| url | http://www.sciencedirect.com/science/article/pii/S2772582023000086 |
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