Molecular and anatomical organization of the dorsal raphe nucleus
The dorsal raphe nucleus (DRN) is an important source of neuromodulators and has been implicated in a wide variety of behavioral and neurological disorders. The DRN is subdivided into distinct anatomical subregions comprised of multiple cell types, and its complex cellular organization has impeded e...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
eLife Sciences Publications Ltd
2019-08-01
|
| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/46464 |
| _version_ | 1811253030499123200 |
|---|---|
| author | Kee Wui Huang Nicole E Ochandarena Adrienne C Philson Minsuk Hyun Jaclyn E Birnbaum Marcelo Cicconet Bernardo L Sabatini |
| author_facet | Kee Wui Huang Nicole E Ochandarena Adrienne C Philson Minsuk Hyun Jaclyn E Birnbaum Marcelo Cicconet Bernardo L Sabatini |
| author_sort | Kee Wui Huang |
| collection | DOAJ |
| description | The dorsal raphe nucleus (DRN) is an important source of neuromodulators and has been implicated in a wide variety of behavioral and neurological disorders. The DRN is subdivided into distinct anatomical subregions comprised of multiple cell types, and its complex cellular organization has impeded efforts to investigate the distinct circuit and behavioral functions of its subdomains. Here we used single-cell RNA sequencing, in situ hybridization, anatomical tracing, and spatial correlation analysis to map the transcriptional and spatial profiles of cells from the mouse DRN. Our analysis of 39,411 single-cell transcriptomes revealed at least 18 distinct neuron subtypes and 5 serotonergic neuron subtypes with distinct molecular and anatomical properties, including a serotonergic neuron subtype that preferentially innervates the basal ganglia. Our study lays out the molecular organization of distinct serotonergic and non-serotonergic subsystems, and will facilitate the design of strategies for further dissection of the DRN and its diverse functions. |
| first_indexed | 2024-04-12T16:43:38Z |
| format | Article |
| id | doaj.art-c50ce8c2d8a44385ab2c694c9b35011d |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T16:43:38Z |
| publishDate | 2019-08-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-c50ce8c2d8a44385ab2c694c9b35011d2022-12-22T03:24:40ZengeLife Sciences Publications LtdeLife2050-084X2019-08-01810.7554/eLife.46464Molecular and anatomical organization of the dorsal raphe nucleusKee Wui Huang0https://orcid.org/0000-0003-2265-4550Nicole E Ochandarena1Adrienne C Philson2Minsuk Hyun3Jaclyn E Birnbaum4Marcelo Cicconet5Bernardo L Sabatini6https://orcid.org/0000-0003-0095-9177Department of Neurobiology, Howard Hughes Medical Institute, Harvard Medical School, Boston, United StatesDepartment of Neurobiology, Howard Hughes Medical Institute, Harvard Medical School, Boston, United StatesDepartment of Neurobiology, Howard Hughes Medical Institute, Harvard Medical School, Boston, United StatesDepartment of Neurobiology, Howard Hughes Medical Institute, Harvard Medical School, Boston, United StatesDepartment of Neurobiology, Howard Hughes Medical Institute, Harvard Medical School, Boston, United StatesImage and Data Analysis Core, Harvard Medical School, Boston, United StatesDepartment of Neurobiology, Howard Hughes Medical Institute, Harvard Medical School, Boston, United StatesThe dorsal raphe nucleus (DRN) is an important source of neuromodulators and has been implicated in a wide variety of behavioral and neurological disorders. The DRN is subdivided into distinct anatomical subregions comprised of multiple cell types, and its complex cellular organization has impeded efforts to investigate the distinct circuit and behavioral functions of its subdomains. Here we used single-cell RNA sequencing, in situ hybridization, anatomical tracing, and spatial correlation analysis to map the transcriptional and spatial profiles of cells from the mouse DRN. Our analysis of 39,411 single-cell transcriptomes revealed at least 18 distinct neuron subtypes and 5 serotonergic neuron subtypes with distinct molecular and anatomical properties, including a serotonergic neuron subtype that preferentially innervates the basal ganglia. Our study lays out the molecular organization of distinct serotonergic and non-serotonergic subsystems, and will facilitate the design of strategies for further dissection of the DRN and its diverse functions.https://elifesciences.org/articles/46464dorsal raphe nucleussingle cell RNAseqserotoninneuromodulation |
| spellingShingle | Kee Wui Huang Nicole E Ochandarena Adrienne C Philson Minsuk Hyun Jaclyn E Birnbaum Marcelo Cicconet Bernardo L Sabatini Molecular and anatomical organization of the dorsal raphe nucleus eLife dorsal raphe nucleus single cell RNAseq serotonin neuromodulation |
| title | Molecular and anatomical organization of the dorsal raphe nucleus |
| title_full | Molecular and anatomical organization of the dorsal raphe nucleus |
| title_fullStr | Molecular and anatomical organization of the dorsal raphe nucleus |
| title_full_unstemmed | Molecular and anatomical organization of the dorsal raphe nucleus |
| title_short | Molecular and anatomical organization of the dorsal raphe nucleus |
| title_sort | molecular and anatomical organization of the dorsal raphe nucleus |
| topic | dorsal raphe nucleus single cell RNAseq serotonin neuromodulation |
| url | https://elifesciences.org/articles/46464 |
| work_keys_str_mv | AT keewuihuang molecularandanatomicalorganizationofthedorsalraphenucleus AT nicoleeochandarena molecularandanatomicalorganizationofthedorsalraphenucleus AT adriennecphilson molecularandanatomicalorganizationofthedorsalraphenucleus AT minsukhyun molecularandanatomicalorganizationofthedorsalraphenucleus AT jaclynebirnbaum molecularandanatomicalorganizationofthedorsalraphenucleus AT marcelocicconet molecularandanatomicalorganizationofthedorsalraphenucleus AT bernardolsabatini molecularandanatomicalorganizationofthedorsalraphenucleus |