ST7 Becomes One of the Most Common Staphylococcus aureus Clones After the COVID-19 Epidemic in the City of Wuhan, China
Jihong Gu,1,* Shucheng Shen,1,* Mengyuan Xiong,1,2 Jin Zhao,1,2 Hongpan Tian,1 Xiao Xiao,1,3 Yirong Li1– 3 1Department of Laboratory Medicine, Zhongnan Hospital of Wuhan University, Wuhan, People’s Republic of China; 2Wuhan Research Center for Infectious Diseases and Tumors of the Ch...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2023-02-01
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| Series: | Infection and Drug Resistance |
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| Online Access: | https://www.dovepress.com/st7-becomes-one-of-the-most-common-staphylococcus-aureus-clones-after--peer-reviewed-fulltext-article-IDR |
| _version_ | 1811165006428897280 |
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| author | Gu J Shen S Xiong M Zhao J Tian H Xiao X Li Y |
| author_facet | Gu J Shen S Xiong M Zhao J Tian H Xiao X Li Y |
| author_sort | Gu J |
| collection | DOAJ |
| description | Jihong Gu,1,* Shucheng Shen,1,* Mengyuan Xiong,1,2 Jin Zhao,1,2 Hongpan Tian,1 Xiao Xiao,1,3 Yirong Li1– 3 1Department of Laboratory Medicine, Zhongnan Hospital of Wuhan University, Wuhan, People’s Republic of China; 2Wuhan Research Center for Infectious Diseases and Tumors of the Chinese Academy of Medical Sciences, Wuhan, People’s Republic of China; 3Hubei Engineering Center for Infectious Disease Prevention, Control and Treatment, Wuhan, People’s Republic of China*These authors contributed equally to this workCorrespondence: Yirong Li, Department of Laboratory Medicine, Zhongnan Hospital of Wuhan University, Wuhan, 430061, People’s Republic of China, Tel +8618602718052, Email liyirong838@163.comBackground: Staphylococcus aureus (S. aureus) was able to rapidly evolve and adapt under the pressure of antibiotics, host immune and environmental change. After Corona Virus Disease 2019 (COVID-19) epidemic in Wuhan, China, a large number of disinfectants were used, which might result in rapid evolution of S. aureus.Methods: A total of 619 S. aureus isolates were collected from Zhongnan Hospital, Wuhan University from 2018 to 2021, including group BEFORE (309 strains collected before COVID-19 pandemic) and group AFTER (310 strains collected after COVID-19 pandemic), for comparing the changes of molecular epidemiology. The molecular characteristics of isolates were analyzed by Multi-locus sequence typing (MLST), spa, chromosomal cassette mec (SCCmec) typing, virulence genes were screened by the PCR, antibiotic susceptibility test was carried out by the VITEK system.Results: Thirty-six sequence types (STs) belonging to 14 clone complexes (CCs) were identified. ST5 was the most prevalent clone in both groups, and ST7, ranking the sixth in group BEFORE, became the second dominant clone in group AFTER (6.5% vs 10.0%), whereas ST239 decreased from the seventh to the fourteenth (5.8% vs 1.9%). ST7 in group AFTER had a higher positive rate of virulence genes, including hlb, fnbB, seb, lukDE, sdrE and the proportion of ST7-t091 MRSA strains increased from 19.1% to 50% compared with group BEFORE. Though no significant difference of MRSA proportion was found between two groups, SCCmec type-III in group AFTER decreased (p< 0.01). Though the rate of multidrug-resistance (MDR) decreased, the virulence genes hlb, hlg, fnbB, seb and pvl carrying rates were significantly elevated in MRSA strains of group AFTER.Conclusion: After COVID-19 pandemic, ST7 becomes one of the predominant S. aureus clones in Wuhan and the carrying rate of SCCmec and virulence genes is on the rise. Therefore, it is essential to strengthen the surveillance of ST7 S. aureus clone.Keywords: Staphylococcus aureus, molecular characterization, antimicrobial resistance, virulence gene, disinfectant |
| first_indexed | 2024-04-10T15:31:38Z |
| format | Article |
| id | doaj.art-c511418b2c3a4d2dbe4ba52638a5c966 |
| institution | Directory Open Access Journal |
| issn | 1178-6973 |
| language | English |
| last_indexed | 2024-04-10T15:31:38Z |
| publishDate | 2023-02-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Infection and Drug Resistance |
| spelling | doaj.art-c511418b2c3a4d2dbe4ba52638a5c9662023-02-13T17:59:03ZengDove Medical PressInfection and Drug Resistance1178-69732023-02-01Volume 1684385281603ST7 Becomes One of the Most Common Staphylococcus aureus Clones After the COVID-19 Epidemic in the City of Wuhan, ChinaGu JShen SXiong MZhao JTian HXiao XLi YJihong Gu,1,* Shucheng Shen,1,* Mengyuan Xiong,1,2 Jin Zhao,1,2 Hongpan Tian,1 Xiao Xiao,1,3 Yirong Li1– 3 1Department of Laboratory Medicine, Zhongnan Hospital of Wuhan University, Wuhan, People’s Republic of China; 2Wuhan Research Center for Infectious Diseases and Tumors of the Chinese Academy of Medical Sciences, Wuhan, People’s Republic of China; 3Hubei Engineering Center for Infectious Disease Prevention, Control and Treatment, Wuhan, People’s Republic of China*These authors contributed equally to this workCorrespondence: Yirong Li, Department of Laboratory Medicine, Zhongnan Hospital of Wuhan University, Wuhan, 430061, People’s Republic of China, Tel +8618602718052, Email liyirong838@163.comBackground: Staphylococcus aureus (S. aureus) was able to rapidly evolve and adapt under the pressure of antibiotics, host immune and environmental change. After Corona Virus Disease 2019 (COVID-19) epidemic in Wuhan, China, a large number of disinfectants were used, which might result in rapid evolution of S. aureus.Methods: A total of 619 S. aureus isolates were collected from Zhongnan Hospital, Wuhan University from 2018 to 2021, including group BEFORE (309 strains collected before COVID-19 pandemic) and group AFTER (310 strains collected after COVID-19 pandemic), for comparing the changes of molecular epidemiology. The molecular characteristics of isolates were analyzed by Multi-locus sequence typing (MLST), spa, chromosomal cassette mec (SCCmec) typing, virulence genes were screened by the PCR, antibiotic susceptibility test was carried out by the VITEK system.Results: Thirty-six sequence types (STs) belonging to 14 clone complexes (CCs) were identified. ST5 was the most prevalent clone in both groups, and ST7, ranking the sixth in group BEFORE, became the second dominant clone in group AFTER (6.5% vs 10.0%), whereas ST239 decreased from the seventh to the fourteenth (5.8% vs 1.9%). ST7 in group AFTER had a higher positive rate of virulence genes, including hlb, fnbB, seb, lukDE, sdrE and the proportion of ST7-t091 MRSA strains increased from 19.1% to 50% compared with group BEFORE. Though no significant difference of MRSA proportion was found between two groups, SCCmec type-III in group AFTER decreased (p< 0.01). Though the rate of multidrug-resistance (MDR) decreased, the virulence genes hlb, hlg, fnbB, seb and pvl carrying rates were significantly elevated in MRSA strains of group AFTER.Conclusion: After COVID-19 pandemic, ST7 becomes one of the predominant S. aureus clones in Wuhan and the carrying rate of SCCmec and virulence genes is on the rise. Therefore, it is essential to strengthen the surveillance of ST7 S. aureus clone.Keywords: Staphylococcus aureus, molecular characterization, antimicrobial resistance, virulence gene, disinfectanthttps://www.dovepress.com/st7-becomes-one-of-the-most-common-staphylococcus-aureus-clones-after--peer-reviewed-fulltext-article-IDRstaphylococcus aureusmolecular characterizationantimicrobial resistancevirulence genedisinfectant |
| spellingShingle | Gu J Shen S Xiong M Zhao J Tian H Xiao X Li Y ST7 Becomes One of the Most Common Staphylococcus aureus Clones After the COVID-19 Epidemic in the City of Wuhan, China Infection and Drug Resistance staphylococcus aureus molecular characterization antimicrobial resistance virulence gene disinfectant |
| title | ST7 Becomes One of the Most Common Staphylococcus aureus Clones After the COVID-19 Epidemic in the City of Wuhan, China |
| title_full | ST7 Becomes One of the Most Common Staphylococcus aureus Clones After the COVID-19 Epidemic in the City of Wuhan, China |
| title_fullStr | ST7 Becomes One of the Most Common Staphylococcus aureus Clones After the COVID-19 Epidemic in the City of Wuhan, China |
| title_full_unstemmed | ST7 Becomes One of the Most Common Staphylococcus aureus Clones After the COVID-19 Epidemic in the City of Wuhan, China |
| title_short | ST7 Becomes One of the Most Common Staphylococcus aureus Clones After the COVID-19 Epidemic in the City of Wuhan, China |
| title_sort | st7 becomes one of the most common staphylococcus aureus clones after the covid 19 epidemic in the city of wuhan china |
| topic | staphylococcus aureus molecular characterization antimicrobial resistance virulence gene disinfectant |
| url | https://www.dovepress.com/st7-becomes-one-of-the-most-common-staphylococcus-aureus-clones-after--peer-reviewed-fulltext-article-IDR |
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