Comparison and Harmonization of Different Semi-Automated and Automated qRT-PCR Assays in the Assessment of SARS-CoV-2

Comparison and Harmonization of Different Semi-Automated and Automated qRT-PCR Assays in the Assessment of SARS-CoV-2

In SARS-CoV-2 diagnostics, cycle threshold (Ct) values from qRT-PCRs semi-quantitatively estimate a patient’s viral load. However, relevant analytical differences between qRT-PCR assays are often neglected. This study was designed (i) to identify such differences between five commonly used assays an...

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Bibliographic Details
Main Authors: Sascha Dierks, Karin Thiele, Wolfgang Bohne, Raimond Lugert, Michael Weig, Uwe Groß, Nicolas von Ahsen, Julie Schanz, Andreas Fischer, Moritz Schnelle
Format: Article
Language:English
Published: MDPI AG 2022-10-01
Series:Viruses
Subjects:
COVID-19
SARS-CoV-2
quantitative reverse transcriptase-polymerase chain reaction
cycle threshold value
viral load
Online Access:https://www.mdpi.com/1999-4915/14/10/2239

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