Optimized ultrasound-assisted recovery, HPLC/LC-MS identification and biological activities of Tetrapleura tetraptera L. dry fruit polyphenols
Little information exists in literature about the optimization of ultrasound-assisted extraction (UAE) of Tetrapleura tetraptera (TT) fruit polyphenols (TTP). The use of conventional methods and lack of literature on the application of green extraction methods limit the study scope and application o...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-10-01
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| Series: | Food Chemistry Advances |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2772753X22000818 |
| _version_ | 1811178535759380480 |
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| author | Courage Sedem Dzah |
| author_facet | Courage Sedem Dzah |
| author_sort | Courage Sedem Dzah |
| collection | DOAJ |
| description | Little information exists in literature about the optimization of ultrasound-assisted extraction (UAE) of Tetrapleura tetraptera (TT) fruit polyphenols (TTP). The use of conventional methods and lack of literature on the application of green extraction methods limit the study scope and application of TT. This study optimized UAE to maximize total phenol content (TPC) and antioxidant activity (AA) of TTP extract. TTP extract was characterized using HPLC/LC-MS. Cellular antioxidant activity (CAA), cytotoxicity and antimicrobial activity of TTP against E. coli, Staph. aureus and B. subtilis were investigated. TPC of 7.044 mg GAE g−1 dw−1 and AA of 66.558% were observed at optimum time, frequency, power and liquid-to-solid ratio of 20 min, 20 kHz, 150 W and 26 mL g−1, respectively. Twenty-four phenolic acids and flavonoids were identified. TTP exhibited high CAA and cytotoxicity in HepG2 liver cancer cells and also showed strong bactericidal activity even at low concentrations. |
| first_indexed | 2024-04-11T06:21:04Z |
| format | Article |
| id | doaj.art-c51577418e4b43ad8e94756f6f3440da |
| institution | Directory Open Access Journal |
| issn | 2772-753X |
| language | English |
| last_indexed | 2024-04-11T06:21:04Z |
| publishDate | 2022-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Food Chemistry Advances |
| spelling | doaj.art-c51577418e4b43ad8e94756f6f3440da2022-12-22T04:40:36ZengElsevierFood Chemistry Advances2772-753X2022-10-011100093Optimized ultrasound-assisted recovery, HPLC/LC-MS identification and biological activities of Tetrapleura tetraptera L. dry fruit polyphenolsCourage Sedem Dzah0Department of Food Science and Technology, Faculty of Applied Sciences and Technology, Ho Technical University, Ho, HP217, GhanaLittle information exists in literature about the optimization of ultrasound-assisted extraction (UAE) of Tetrapleura tetraptera (TT) fruit polyphenols (TTP). The use of conventional methods and lack of literature on the application of green extraction methods limit the study scope and application of TT. This study optimized UAE to maximize total phenol content (TPC) and antioxidant activity (AA) of TTP extract. TTP extract was characterized using HPLC/LC-MS. Cellular antioxidant activity (CAA), cytotoxicity and antimicrobial activity of TTP against E. coli, Staph. aureus and B. subtilis were investigated. TPC of 7.044 mg GAE g−1 dw−1 and AA of 66.558% were observed at optimum time, frequency, power and liquid-to-solid ratio of 20 min, 20 kHz, 150 W and 26 mL g−1, respectively. Twenty-four phenolic acids and flavonoids were identified. TTP exhibited high CAA and cytotoxicity in HepG2 liver cancer cells and also showed strong bactericidal activity even at low concentrations.http://www.sciencedirect.com/science/article/pii/S2772753X22000818Ultrasound assisted extractionAntimicrobial activityTetrapleura tetrapteraPolyphenolsAntioxidant activityGreen extraction |
| spellingShingle | Courage Sedem Dzah Optimized ultrasound-assisted recovery, HPLC/LC-MS identification and biological activities of Tetrapleura tetraptera L. dry fruit polyphenols Food Chemistry Advances Ultrasound assisted extraction Antimicrobial activity Tetrapleura tetraptera Polyphenols Antioxidant activity Green extraction |
| title | Optimized ultrasound-assisted recovery, HPLC/LC-MS identification and biological activities of Tetrapleura tetraptera L. dry fruit polyphenols |
| title_full | Optimized ultrasound-assisted recovery, HPLC/LC-MS identification and biological activities of Tetrapleura tetraptera L. dry fruit polyphenols |
| title_fullStr | Optimized ultrasound-assisted recovery, HPLC/LC-MS identification and biological activities of Tetrapleura tetraptera L. dry fruit polyphenols |
| title_full_unstemmed | Optimized ultrasound-assisted recovery, HPLC/LC-MS identification and biological activities of Tetrapleura tetraptera L. dry fruit polyphenols |
| title_short | Optimized ultrasound-assisted recovery, HPLC/LC-MS identification and biological activities of Tetrapleura tetraptera L. dry fruit polyphenols |
| title_sort | optimized ultrasound assisted recovery hplc lc ms identification and biological activities of tetrapleura tetraptera l dry fruit polyphenols |
| topic | Ultrasound assisted extraction Antimicrobial activity Tetrapleura tetraptera Polyphenols Antioxidant activity Green extraction |
| url | http://www.sciencedirect.com/science/article/pii/S2772753X22000818 |
| work_keys_str_mv | AT couragesedemdzah optimizedultrasoundassistedrecoveryhplclcmsidentificationandbiologicalactivitiesoftetrapleuratetrapteraldryfruitpolyphenols |