Temperature-Biased miRNA Expression Patterns during European Sea Bass (<i>Dicentrarchus labrax</i>) Development
Environmental effects and, particularly, temperature changes have been demonstrated to influence the activity, function, and well-being of teleosts. Temperature may change seasonally in the wild, and in captivity under aquaculture operations. Moreover, climate change is expected to shift temperature...
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MDPI AG
2022-09-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/19/11164 |
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| author | Maria Papadaki Elisavet Kaitetzidou Ioannis E. Papadakis Dimitris G. Sfakianakis Nikos Papandroulakis Constantinos C. Mylonas Elena Sarropoulou |
| author_facet | Maria Papadaki Elisavet Kaitetzidou Ioannis E. Papadakis Dimitris G. Sfakianakis Nikos Papandroulakis Constantinos C. Mylonas Elena Sarropoulou |
| author_sort | Maria Papadaki |
| collection | DOAJ |
| description | Environmental effects and, particularly, temperature changes have been demonstrated to influence the activity, function, and well-being of teleosts. Temperature may change seasonally in the wild, and in captivity under aquaculture operations. Moreover, climate change is expected to shift temperature profiles worldwide. MicroRNAs (miRNA) are important temperature-sensitive gene-expression regulators acting at the post-transcriptional level. They are known to be key regulators in development, reproduction, and immune responses. Therefore, early larval development of the European sea bass (<i>Dicentrarchus labrax</i>), one of the most extensively cultured species in Mediterranean aquaculture, was investigated at early rearing temperatures, i.e., 15, 17.5, and 20 °C, in regard to the impact of temperatures on miRNAs through sncRNA high-throughput sequencing but also at the phenotypic level in terms of growth, sex, vision, and skeletal deformities. Expression profiling revealed stage- and temperature-specific miRNA expression targeting genes with roles in reproduction and immune response mainly at the flexion and all-fins stages. Similar stage- and temperature-specific results were also observed concerning the number of rod cells and lower jaw elongation. The present work presents for the first time highly promising results on the influence of early rearing temperature at the post-transcriptional level during European sea bass development, with a putative impact on reproduction and immune response, as well as regarding teleost vision and larval development. |
| first_indexed | 2024-03-09T21:42:33Z |
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| id | doaj.art-c51ba1eff9fe4d588b676dff361d2ed4 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T21:42:33Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-c51ba1eff9fe4d588b676dff361d2ed42023-11-23T20:28:21ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-09-0123191116410.3390/ijms231911164Temperature-Biased miRNA Expression Patterns during European Sea Bass (<i>Dicentrarchus labrax</i>) DevelopmentMaria Papadaki0Elisavet Kaitetzidou1Ioannis E. Papadakis2Dimitris G. Sfakianakis3Nikos Papandroulakis4Constantinos C. Mylonas5Elena Sarropoulou6Institute of Marine Biology, Biotechnology, and Aquaculture, Hellenic Centre for Marine Research, 71003 Heraklion, Crete, GreeceInstitute of Marine Biology, Biotechnology, and Aquaculture, Hellenic Centre for Marine Research, 71003 Heraklion, Crete, GreeceInstitute of Marine Biology, Biotechnology, and Aquaculture, Hellenic Centre for Marine Research, 71003 Heraklion, Crete, GreeceDepartment of Biology, University of Crete, 71409 Heraklion, Crete, GreeceInstitute of Marine Biology, Biotechnology, and Aquaculture, Hellenic Centre for Marine Research, 71003 Heraklion, Crete, GreeceInstitute of Marine Biology, Biotechnology, and Aquaculture, Hellenic Centre for Marine Research, 71003 Heraklion, Crete, GreeceInstitute of Marine Biology, Biotechnology, and Aquaculture, Hellenic Centre for Marine Research, 71003 Heraklion, Crete, GreeceEnvironmental effects and, particularly, temperature changes have been demonstrated to influence the activity, function, and well-being of teleosts. Temperature may change seasonally in the wild, and in captivity under aquaculture operations. Moreover, climate change is expected to shift temperature profiles worldwide. MicroRNAs (miRNA) are important temperature-sensitive gene-expression regulators acting at the post-transcriptional level. They are known to be key regulators in development, reproduction, and immune responses. Therefore, early larval development of the European sea bass (<i>Dicentrarchus labrax</i>), one of the most extensively cultured species in Mediterranean aquaculture, was investigated at early rearing temperatures, i.e., 15, 17.5, and 20 °C, in regard to the impact of temperatures on miRNAs through sncRNA high-throughput sequencing but also at the phenotypic level in terms of growth, sex, vision, and skeletal deformities. Expression profiling revealed stage- and temperature-specific miRNA expression targeting genes with roles in reproduction and immune response mainly at the flexion and all-fins stages. Similar stage- and temperature-specific results were also observed concerning the number of rod cells and lower jaw elongation. The present work presents for the first time highly promising results on the influence of early rearing temperature at the post-transcriptional level during European sea bass development, with a putative impact on reproduction and immune response, as well as regarding teleost vision and larval development.https://www.mdpi.com/1422-0067/23/19/11164temperaturemiRNAEuropean sea bassdevelopmentgene expressionphenotypes |
| spellingShingle | Maria Papadaki Elisavet Kaitetzidou Ioannis E. Papadakis Dimitris G. Sfakianakis Nikos Papandroulakis Constantinos C. Mylonas Elena Sarropoulou Temperature-Biased miRNA Expression Patterns during European Sea Bass (<i>Dicentrarchus labrax</i>) Development International Journal of Molecular Sciences temperature miRNA European sea bass development gene expression phenotypes |
| title | Temperature-Biased miRNA Expression Patterns during European Sea Bass (<i>Dicentrarchus labrax</i>) Development |
| title_full | Temperature-Biased miRNA Expression Patterns during European Sea Bass (<i>Dicentrarchus labrax</i>) Development |
| title_fullStr | Temperature-Biased miRNA Expression Patterns during European Sea Bass (<i>Dicentrarchus labrax</i>) Development |
| title_full_unstemmed | Temperature-Biased miRNA Expression Patterns during European Sea Bass (<i>Dicentrarchus labrax</i>) Development |
| title_short | Temperature-Biased miRNA Expression Patterns during European Sea Bass (<i>Dicentrarchus labrax</i>) Development |
| title_sort | temperature biased mirna expression patterns during european sea bass i dicentrarchus labrax i development |
| topic | temperature miRNA European sea bass development gene expression phenotypes |
| url | https://www.mdpi.com/1422-0067/23/19/11164 |
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