ppGpp signaling plays a critical role in virulence of Acinetobacter baumannii
Acinetobacter baumannii, a major nosocomial pathogen, survives in diverse hospital environments, and its multidrug resistance is a major concern. The ppGpp-dependent stringent response mediates the reprogramming of genes with diverse functions in several bacteria. We investigated whether ppGpp is in...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2021-12-01
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| Series: | Virulence |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/21505594.2021.1961660 |
| _version_ | 1798014867087556608 |
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| author | Kyeongmin Kim Maidul Islam Hye-won Jung Daejin Lim Kwangsoo Kim Sung-Gwon Lee Chungoo Park Je Chul Lee Minsang Shin |
| author_facet | Kyeongmin Kim Maidul Islam Hye-won Jung Daejin Lim Kwangsoo Kim Sung-Gwon Lee Chungoo Park Je Chul Lee Minsang Shin |
| author_sort | Kyeongmin Kim |
| collection | DOAJ |
| description | Acinetobacter baumannii, a major nosocomial pathogen, survives in diverse hospital environments, and its multidrug resistance is a major concern. The ppGpp-dependent stringent response mediates the reprogramming of genes with diverse functions in several bacteria. We investigated whether ppGpp is involved in A. baumannii’s pathogenesis by examining biofilm formation, surface motility, adhesion, invasion, and mouse infection studies. Transcriptome analysis of early stationary phase cultures revealed 498 differentially-expressed genes (≥ 2-fold change) in a ppGpp-deficient A. baumannii strain; 220 and 278 genes were up and downregulated, respectively. Csu operon expression, important in pilus biosynthesis during early biofilm formation, was significantly reduced in the ppGpp-deficient strain. Our findings suggest that ppGpp signaling influences A. baumannii biofilm formation, surface motility, adherence, and virulence. We showed the association between ppGpp and pathogenicity in A. baumannii for the first time; ppGpp may be a novel antivirulence target in A. baumannii. |
| first_indexed | 2024-04-11T15:24:00Z |
| format | Article |
| id | doaj.art-c524af68c1184bbd8cf00215d78779a0 |
| institution | Directory Open Access Journal |
| issn | 2150-5594 2150-5608 |
| language | English |
| last_indexed | 2024-04-11T15:24:00Z |
| publishDate | 2021-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Virulence |
| spelling | doaj.art-c524af68c1184bbd8cf00215d78779a02022-12-22T04:16:18ZengTaylor & Francis GroupVirulence2150-55942150-56082021-12-011212122213210.1080/21505594.2021.19616601961660ppGpp signaling plays a critical role in virulence of Acinetobacter baumanniiKyeongmin Kim0Maidul Islam1Hye-won Jung2Daejin Lim3Kwangsoo Kim4Sung-Gwon Lee5Chungoo Park6Je Chul Lee7Minsang Shin8School of Medicine, Kyungpook National UniversitySchool of Medicine, Kyungpook National UniversitySchool of Medicine, Kyungpook National UniversityChonnam National University Medical SchoolChonnam National University Medical SchoolSchool of Biological Sciences and Technology, Chonnam National UniversitySchool of Biological Sciences and Technology, Chonnam National UniversitySchool of Medicine, Kyungpook National UniversitySchool of Medicine, Kyungpook National UniversityAcinetobacter baumannii, a major nosocomial pathogen, survives in diverse hospital environments, and its multidrug resistance is a major concern. The ppGpp-dependent stringent response mediates the reprogramming of genes with diverse functions in several bacteria. We investigated whether ppGpp is involved in A. baumannii’s pathogenesis by examining biofilm formation, surface motility, adhesion, invasion, and mouse infection studies. Transcriptome analysis of early stationary phase cultures revealed 498 differentially-expressed genes (≥ 2-fold change) in a ppGpp-deficient A. baumannii strain; 220 and 278 genes were up and downregulated, respectively. Csu operon expression, important in pilus biosynthesis during early biofilm formation, was significantly reduced in the ppGpp-deficient strain. Our findings suggest that ppGpp signaling influences A. baumannii biofilm formation, surface motility, adherence, and virulence. We showed the association between ppGpp and pathogenicity in A. baumannii for the first time; ppGpp may be a novel antivirulence target in A. baumannii.http://dx.doi.org/10.1080/21505594.2021.1961660acinetobacter baumanniippgpptranscriptomebiofilmvirulence |
| spellingShingle | Kyeongmin Kim Maidul Islam Hye-won Jung Daejin Lim Kwangsoo Kim Sung-Gwon Lee Chungoo Park Je Chul Lee Minsang Shin ppGpp signaling plays a critical role in virulence of Acinetobacter baumannii Virulence acinetobacter baumannii ppgpp transcriptome biofilm virulence |
| title | ppGpp signaling plays a critical role in virulence of Acinetobacter baumannii |
| title_full | ppGpp signaling plays a critical role in virulence of Acinetobacter baumannii |
| title_fullStr | ppGpp signaling plays a critical role in virulence of Acinetobacter baumannii |
| title_full_unstemmed | ppGpp signaling plays a critical role in virulence of Acinetobacter baumannii |
| title_short | ppGpp signaling plays a critical role in virulence of Acinetobacter baumannii |
| title_sort | ppgpp signaling plays a critical role in virulence of acinetobacter baumannii |
| topic | acinetobacter baumannii ppgpp transcriptome biofilm virulence |
| url | http://dx.doi.org/10.1080/21505594.2021.1961660 |
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