Gradient-temperature hot-embossing for dense micropillar array fabrication on thick cyclo-olefin polymeric plates: An example of a microfluidic chromatography column fabrication
The fabrication of dense and high aspect ratio pillar arrays is important for several applications. In particular polymeric microfluidic chromatography columns are of high importance for various biological, pharmaceutical and chemical separations. Here, a cyclo‐olefin polymeric (COP) microcolumn fab...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2019-12-01
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| Series: | Micro and Nano Engineering |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590007219300279 |
| _version_ | 1819265264324182016 |
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| author | Ioannis Kourmpetis Athina S. Kastania Kosmas Ellinas Katerina Tsougeni Martyna Baca Wim De Malsche Evangelos Gogolides |
| author_facet | Ioannis Kourmpetis Athina S. Kastania Kosmas Ellinas Katerina Tsougeni Martyna Baca Wim De Malsche Evangelos Gogolides |
| author_sort | Ioannis Kourmpetis |
| collection | DOAJ |
| description | The fabrication of dense and high aspect ratio pillar arrays is important for several applications. In particular polymeric microfluidic chromatography columns are of high importance for various biological, pharmaceutical and chemical separations. Here, a cyclo‐olefin polymeric (COP) microcolumn fabrication process is developed using hot embossing with a temperature gradient for replication of a fine and highly dense array of micropillars on the surface of a 2 mm thick polymer plate without chip deformation. The design consists of an array of ordered cylindrical pillars with 15 μm diameter, 4 μm interpillar, and 2 μm pillar-wall distance with 20 μm height. The produced microcolumn is subsequently sealed with thermal bonding in a laminator and tested for liquid pressure operation up to 20 bar. Since COPs are hydrophobic by nature and have been used for reversed-phase liquid chromatographic (RPLC) separations in the past, preliminary separation experiments are also demonstrated in the fabricated column. Keywords: Cyclo-olefin polymer (COP), Hot embossing, Microcolumn, Thermal bonding, Reversed phase chromatography |
| first_indexed | 2024-12-23T20:42:37Z |
| format | Article |
| id | doaj.art-c5264112b5d8498db83537af6dc0bf45 |
| institution | Directory Open Access Journal |
| issn | 2590-0072 |
| language | English |
| last_indexed | 2024-12-23T20:42:37Z |
| publishDate | 2019-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Micro and Nano Engineering |
| spelling | doaj.art-c5264112b5d8498db83537af6dc0bf452022-12-21T17:31:52ZengElsevierMicro and Nano Engineering2590-00722019-12-015Gradient-temperature hot-embossing for dense micropillar array fabrication on thick cyclo-olefin polymeric plates: An example of a microfluidic chromatography column fabricationIoannis Kourmpetis0Athina S. Kastania1Kosmas Ellinas2Katerina Tsougeni3Martyna Baca4Wim De Malsche5Evangelos Gogolides6Institute of Nanoscience and Nanotechnology, NCSR “Demokritos”, Patriarhou Gregoriou and Neapoleos 27, 153 10 Aghia Paraskevi, GreeceInstitute of Nanoscience and Nanotechnology, NCSR “Demokritos”, Patriarhou Gregoriou and Neapoleos 27, 153 10 Aghia Paraskevi, Greece; Department of Chemistry, National and Kapodistrian University of Athens, 157 71 Athens, GreeceInstitute of Nanoscience and Nanotechnology, NCSR “Demokritos”, Patriarhou Gregoriou and Neapoleos 27, 153 10 Aghia Paraskevi, GreeceInstitute of Nanoscience and Nanotechnology, NCSR “Demokritos”, Patriarhou Gregoriou and Neapoleos 27, 153 10 Aghia Paraskevi, GreeceVrije Universiteit Brussel, Pleinlaan 2, B1050, BelgiumVrije Universiteit Brussel, Pleinlaan 2, B1050, BelgiumInstitute of Nanoscience and Nanotechnology, NCSR “Demokritos”, Patriarhou Gregoriou and Neapoleos 27, 153 10 Aghia Paraskevi, Greece; Corresponding author.The fabrication of dense and high aspect ratio pillar arrays is important for several applications. In particular polymeric microfluidic chromatography columns are of high importance for various biological, pharmaceutical and chemical separations. Here, a cyclo‐olefin polymeric (COP) microcolumn fabrication process is developed using hot embossing with a temperature gradient for replication of a fine and highly dense array of micropillars on the surface of a 2 mm thick polymer plate without chip deformation. The design consists of an array of ordered cylindrical pillars with 15 μm diameter, 4 μm interpillar, and 2 μm pillar-wall distance with 20 μm height. The produced microcolumn is subsequently sealed with thermal bonding in a laminator and tested for liquid pressure operation up to 20 bar. Since COPs are hydrophobic by nature and have been used for reversed-phase liquid chromatographic (RPLC) separations in the past, preliminary separation experiments are also demonstrated in the fabricated column. Keywords: Cyclo-olefin polymer (COP), Hot embossing, Microcolumn, Thermal bonding, Reversed phase chromatographyhttp://www.sciencedirect.com/science/article/pii/S2590007219300279 |
| spellingShingle | Ioannis Kourmpetis Athina S. Kastania Kosmas Ellinas Katerina Tsougeni Martyna Baca Wim De Malsche Evangelos Gogolides Gradient-temperature hot-embossing for dense micropillar array fabrication on thick cyclo-olefin polymeric plates: An example of a microfluidic chromatography column fabrication Micro and Nano Engineering |
| title | Gradient-temperature hot-embossing for dense micropillar array fabrication on thick cyclo-olefin polymeric plates: An example of a microfluidic chromatography column fabrication |
| title_full | Gradient-temperature hot-embossing for dense micropillar array fabrication on thick cyclo-olefin polymeric plates: An example of a microfluidic chromatography column fabrication |
| title_fullStr | Gradient-temperature hot-embossing for dense micropillar array fabrication on thick cyclo-olefin polymeric plates: An example of a microfluidic chromatography column fabrication |
| title_full_unstemmed | Gradient-temperature hot-embossing for dense micropillar array fabrication on thick cyclo-olefin polymeric plates: An example of a microfluidic chromatography column fabrication |
| title_short | Gradient-temperature hot-embossing for dense micropillar array fabrication on thick cyclo-olefin polymeric plates: An example of a microfluidic chromatography column fabrication |
| title_sort | gradient temperature hot embossing for dense micropillar array fabrication on thick cyclo olefin polymeric plates an example of a microfluidic chromatography column fabrication |
| url | http://www.sciencedirect.com/science/article/pii/S2590007219300279 |
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