Transcriptomic Profiling and Gene Disruption Revealed that Two Genes Related to PUFAs/DHA Biosynthesis May be Essential for Cell Growth of Aurantiochytrium sp.
Aurantiochytrium sp. PKU#SW7 is a thraustochytrid strain that was found to exhibit high potential for docosahexaenoic acid (DHA, C22:6n-3) production. In this work, the transcriptome of Aurantiochytrium sp. PKU#SW7 was analyzed for the study of genes involved in basic metabolic functions and especia...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2018-09-01
|
| Series: | Marine Drugs |
| Subjects: | |
| Online Access: | http://www.mdpi.com/1660-3397/16/9/310 |
| _version_ | 1797999500373000192 |
|---|---|
| author | Yuanmei Liang Ying Liu Jie Tang Jiong Ma Jay J. Cheng Maurycy Daroch |
| author_facet | Yuanmei Liang Ying Liu Jie Tang Jiong Ma Jay J. Cheng Maurycy Daroch |
| author_sort | Yuanmei Liang |
| collection | DOAJ |
| description | Aurantiochytrium sp. PKU#SW7 is a thraustochytrid strain that was found to exhibit high potential for docosahexaenoic acid (DHA, C22:6n-3) production. In this work, the transcriptome of Aurantiochytrium sp. PKU#SW7 was analyzed for the study of genes involved in basic metabolic functions and especially in the mechanisms of DHA biosynthesis. Sequence annotation and functional analysis revealed that the strain contains components of fatty acid synthesis (FAS) and polyketide synthase (PKS) pathways. Fatty acid desaturases and elongases were identified as components of FAS pathway, whilst key components of PKS pathway were also found in the cDNA library. The relative contribution of the two pathways to the synthesis of DHA was unknown, as both pathways appeared to be lacking full complement of genes for standalone synthesis of DHA. Further analysis of two putative genes encoding the very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase and dehydrase/isomerase involved in FAS and PKS pathways, respectively, revealed that under various salinity conditions, their relative expression levels changed corresponding to the variation of DHA content in Aurantiochytrium sp. Independent knock outs of these genes in Aurantiochytrium sp. resulted in poor cell growth, probably due to little or no intracellular DHA accumulation. Hence, it can be speculated that both genes are engaged in DHA biosynthesis and DHA in Aurantiochytrium sp. could be produced by jointed actions of both FAS and PKS systems. |
| first_indexed | 2024-04-11T11:04:23Z |
| format | Article |
| id | doaj.art-c5269ebf2edd4b349d202f20f0fb3726 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-04-11T11:04:23Z |
| publishDate | 2018-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-c5269ebf2edd4b349d202f20f0fb37262022-12-22T04:28:24ZengMDPI AGMarine Drugs1660-33972018-09-0116931010.3390/md16090310md16090310Transcriptomic Profiling and Gene Disruption Revealed that Two Genes Related to PUFAs/DHA Biosynthesis May be Essential for Cell Growth of Aurantiochytrium sp.Yuanmei Liang0Ying Liu1Jie Tang2Jiong Ma3Jay J. Cheng4Maurycy Daroch5School of Environment and Energy, Peking University Shenzhen Graduate School, Shenzhen 518055, ChinaGuangdong Engineering Research Centre for Marine Algal Biotechnology, Shenzhen Key Laboratory of Marine Bioresource and Eco-environmental Science, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518060, ChinaSchool of Pharmacy and Bioengineering, Chengdu University, Chengdu 610106, ChinaSchool of Environment and Energy, Peking University Shenzhen Graduate School, Shenzhen 518055, ChinaSchool of Environment and Energy, Peking University Shenzhen Graduate School, Shenzhen 518055, ChinaSchool of Environment and Energy, Peking University Shenzhen Graduate School, Shenzhen 518055, ChinaAurantiochytrium sp. PKU#SW7 is a thraustochytrid strain that was found to exhibit high potential for docosahexaenoic acid (DHA, C22:6n-3) production. In this work, the transcriptome of Aurantiochytrium sp. PKU#SW7 was analyzed for the study of genes involved in basic metabolic functions and especially in the mechanisms of DHA biosynthesis. Sequence annotation and functional analysis revealed that the strain contains components of fatty acid synthesis (FAS) and polyketide synthase (PKS) pathways. Fatty acid desaturases and elongases were identified as components of FAS pathway, whilst key components of PKS pathway were also found in the cDNA library. The relative contribution of the two pathways to the synthesis of DHA was unknown, as both pathways appeared to be lacking full complement of genes for standalone synthesis of DHA. Further analysis of two putative genes encoding the very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase and dehydrase/isomerase involved in FAS and PKS pathways, respectively, revealed that under various salinity conditions, their relative expression levels changed corresponding to the variation of DHA content in Aurantiochytrium sp. Independent knock outs of these genes in Aurantiochytrium sp. resulted in poor cell growth, probably due to little or no intracellular DHA accumulation. Hence, it can be speculated that both genes are engaged in DHA biosynthesis and DHA in Aurantiochytrium sp. could be produced by jointed actions of both FAS and PKS systems.http://www.mdpi.com/1660-3397/16/9/310thraustochytridAurantiochytriumtranscriptomevery-long-chain (3R)-3-hydroxyacyl-CoA dehydratasedehydrase/isomerase |
| spellingShingle | Yuanmei Liang Ying Liu Jie Tang Jiong Ma Jay J. Cheng Maurycy Daroch Transcriptomic Profiling and Gene Disruption Revealed that Two Genes Related to PUFAs/DHA Biosynthesis May be Essential for Cell Growth of Aurantiochytrium sp. Marine Drugs thraustochytrid Aurantiochytrium transcriptome very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase dehydrase/isomerase |
| title | Transcriptomic Profiling and Gene Disruption Revealed that Two Genes Related to PUFAs/DHA Biosynthesis May be Essential for Cell Growth of Aurantiochytrium sp. |
| title_full | Transcriptomic Profiling and Gene Disruption Revealed that Two Genes Related to PUFAs/DHA Biosynthesis May be Essential for Cell Growth of Aurantiochytrium sp. |
| title_fullStr | Transcriptomic Profiling and Gene Disruption Revealed that Two Genes Related to PUFAs/DHA Biosynthesis May be Essential for Cell Growth of Aurantiochytrium sp. |
| title_full_unstemmed | Transcriptomic Profiling and Gene Disruption Revealed that Two Genes Related to PUFAs/DHA Biosynthesis May be Essential for Cell Growth of Aurantiochytrium sp. |
| title_short | Transcriptomic Profiling and Gene Disruption Revealed that Two Genes Related to PUFAs/DHA Biosynthesis May be Essential for Cell Growth of Aurantiochytrium sp. |
| title_sort | transcriptomic profiling and gene disruption revealed that two genes related to pufas dha biosynthesis may be essential for cell growth of aurantiochytrium sp |
| topic | thraustochytrid Aurantiochytrium transcriptome very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase dehydrase/isomerase |
| url | http://www.mdpi.com/1660-3397/16/9/310 |
| work_keys_str_mv | AT yuanmeiliang transcriptomicprofilingandgenedisruptionrevealedthattwogenesrelatedtopufasdhabiosynthesismaybeessentialforcellgrowthofaurantiochytriumsp AT yingliu transcriptomicprofilingandgenedisruptionrevealedthattwogenesrelatedtopufasdhabiosynthesismaybeessentialforcellgrowthofaurantiochytriumsp AT jietang transcriptomicprofilingandgenedisruptionrevealedthattwogenesrelatedtopufasdhabiosynthesismaybeessentialforcellgrowthofaurantiochytriumsp AT jiongma transcriptomicprofilingandgenedisruptionrevealedthattwogenesrelatedtopufasdhabiosynthesismaybeessentialforcellgrowthofaurantiochytriumsp AT jayjcheng transcriptomicprofilingandgenedisruptionrevealedthattwogenesrelatedtopufasdhabiosynthesismaybeessentialforcellgrowthofaurantiochytriumsp AT maurycydaroch transcriptomicprofilingandgenedisruptionrevealedthattwogenesrelatedtopufasdhabiosynthesismaybeessentialforcellgrowthofaurantiochytriumsp |