Application of LC-MS/MS MRM to Determine Staphylococcal Enterotoxins (SEB and SEA) in Milk
Staphylococcus aureus is one of the important aetiological agents of food intoxications in Europe and can cause gastro-enteritis through the production of various staphylococcal enterotoxins (SEs) in foods. Due to their stability and ease of production and dissemination, some SEs have also been stud...
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| Format: | Article |
| Language: | English |
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MDPI AG
2016-04-01
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| Series: | Toxins |
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| Online Access: | http://www.mdpi.com/2072-6651/8/4/118 |
| _version_ | 1798041025857454080 |
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| author | Mirjana Andjelkovic Varvara Tsilia Andreja Rajkovic Koen De Cremer Joris Van Loco |
| author_facet | Mirjana Andjelkovic Varvara Tsilia Andreja Rajkovic Koen De Cremer Joris Van Loco |
| author_sort | Mirjana Andjelkovic |
| collection | DOAJ |
| description | Staphylococcus aureus is one of the important aetiological agents of food intoxications in Europe and can cause gastro-enteritis through the production of various staphylococcal enterotoxins (SEs) in foods. Due to their stability and ease of production and dissemination, some SEs have also been studied as potential agents for bioterrorism. Therefore, specific and accurate analytical tools are required to detect and quantify SEs. Online solid-phase extraction liquid chromatography electrospray ionization tandem mass spectrometry (online SPE-LC-ESI-MS/MS) based on multiple reaction monitoring (MRM) was used to detect and quantify two types of SE (A and B) spiked in milk and buffer solution. SE extraction and concentration was performed according to the European Screening Method developed by the European Reference Laboratory for Coagulase Positive Staphylococci. Trypsin digests were screened for the presence of SEs using selected proteotypic heavy-labeled peptides as internal standards. SEA and SEB were successfully detected in milk samples using LC-MS/MS in MRM mode. The selected SE peptides were proteotypic for each toxin, allowing the discrimination of SEA and SEB in a single run. The detection limit of SEA and SEB was approximately 8 and 4 ng/g, respectively. |
| first_indexed | 2024-04-11T22:15:44Z |
| format | Article |
| id | doaj.art-c52bc2fca28d40e397de5f46b3059ea3 |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-04-11T22:15:44Z |
| publishDate | 2016-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-c52bc2fca28d40e397de5f46b3059ea32022-12-22T04:00:23ZengMDPI AGToxins2072-66512016-04-018411810.3390/toxins8040118toxins8040118Application of LC-MS/MS MRM to Determine Staphylococcal Enterotoxins (SEB and SEA) in MilkMirjana Andjelkovic0Varvara Tsilia1Andreja Rajkovic2Koen De Cremer3Joris Van Loco4Food, Medicine and Consumer Safety, Scientific Institute of Public Health (WIV-ISP), Juliette Wytsmanstraat 14, 1050 Brussels, BelgiumFood, Medicine and Consumer Safety, Scientific Institute of Public Health (WIV-ISP), Juliette Wytsmanstraat 14, 1050 Brussels, BelgiumLaboratory of Food Microbiology and Food Preservation, Ghent University (UGent), Coupure Links 657, 9000 Ghent, BelgiumFood, Medicine and Consumer Safety, Scientific Institute of Public Health (WIV-ISP), Juliette Wytsmanstraat 14, 1050 Brussels, BelgiumFood, Medicine and Consumer Safety, Scientific Institute of Public Health (WIV-ISP), Juliette Wytsmanstraat 14, 1050 Brussels, BelgiumStaphylococcus aureus is one of the important aetiological agents of food intoxications in Europe and can cause gastro-enteritis through the production of various staphylococcal enterotoxins (SEs) in foods. Due to their stability and ease of production and dissemination, some SEs have also been studied as potential agents for bioterrorism. Therefore, specific and accurate analytical tools are required to detect and quantify SEs. Online solid-phase extraction liquid chromatography electrospray ionization tandem mass spectrometry (online SPE-LC-ESI-MS/MS) based on multiple reaction monitoring (MRM) was used to detect and quantify two types of SE (A and B) spiked in milk and buffer solution. SE extraction and concentration was performed according to the European Screening Method developed by the European Reference Laboratory for Coagulase Positive Staphylococci. Trypsin digests were screened for the presence of SEs using selected proteotypic heavy-labeled peptides as internal standards. SEA and SEB were successfully detected in milk samples using LC-MS/MS in MRM mode. The selected SE peptides were proteotypic for each toxin, allowing the discrimination of SEA and SEB in a single run. The detection limit of SEA and SEB was approximately 8 and 4 ng/g, respectively.http://www.mdpi.com/2072-6651/8/4/118Staphylococcus aureus enterotoxinsSEASEBmilkUPLC-ESI-MS/MS |
| spellingShingle | Mirjana Andjelkovic Varvara Tsilia Andreja Rajkovic Koen De Cremer Joris Van Loco Application of LC-MS/MS MRM to Determine Staphylococcal Enterotoxins (SEB and SEA) in Milk Toxins Staphylococcus aureus enterotoxins SEA SEB milk UPLC-ESI-MS/MS |
| title | Application of LC-MS/MS MRM to Determine Staphylococcal Enterotoxins (SEB and SEA) in Milk |
| title_full | Application of LC-MS/MS MRM to Determine Staphylococcal Enterotoxins (SEB and SEA) in Milk |
| title_fullStr | Application of LC-MS/MS MRM to Determine Staphylococcal Enterotoxins (SEB and SEA) in Milk |
| title_full_unstemmed | Application of LC-MS/MS MRM to Determine Staphylococcal Enterotoxins (SEB and SEA) in Milk |
| title_short | Application of LC-MS/MS MRM to Determine Staphylococcal Enterotoxins (SEB and SEA) in Milk |
| title_sort | application of lc ms ms mrm to determine staphylococcal enterotoxins seb and sea in milk |
| topic | Staphylococcus aureus enterotoxins SEA SEB milk UPLC-ESI-MS/MS |
| url | http://www.mdpi.com/2072-6651/8/4/118 |
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