Research and Application Progress in Visualized RPA-LFD Nucleic Acid Detection Technology
Recombinase polymerase amplification (RPA) is a novel isothermal nucleic acid amplification technology. Compared to PCR, it features ease of use, high efficiency, high sensitivity, high specificity, and does not need specific instruments, which allows it as an alternative to PCR and the most promisi...
| Main Authors: | , , |
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| Format: | Article |
| Language: | zho |
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Editorial Office of Laboratory Animal and Comparative Medicine
2021-12-01
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| Series: | Shiyan dongwu yu bijiao yixue |
| Subjects: | |
| Online Access: | https://www.slarc.org.cn/dwyx/CN/10.12300/j.issn.1674-5817.2021.019 |
| _version_ | 1811215804186755072 |
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| author | YU Lingzhi TAO Lingyun WEI Xiaofeng |
| author_facet | YU Lingzhi TAO Lingyun WEI Xiaofeng |
| author_sort | YU Lingzhi |
| collection | DOAJ |
| description | Recombinase polymerase amplification (RPA) is a novel isothermal nucleic acid amplification technology. Compared to PCR, it features ease of use, high efficiency, high sensitivity, high specificity, and does not need specific instruments, which allows it as an alternative to PCR and the most promising tool for rapid molecular diagnosis. RPA combined with lateral flow dipstick (LFD) (RPA-LFD) enables visual detection of amplified products and has promising applications for rapid nucleic acid detection of pathogens on site. The novel technique provides a new method for quality supervision and inspection of laboratory animals. In this paper, we reviewed the principle, research status, and technical difficulties of RPA-LFD, as well as the progress in rapid extraction of nucleic acid on site. |
| first_indexed | 2024-04-12T06:28:32Z |
| format | Article |
| id | doaj.art-c52ddedd8e8e4e9d8a1b197fc8dcef30 |
| institution | Directory Open Access Journal |
| issn | 1674-5817 |
| language | zho |
| last_indexed | 2024-04-12T06:28:32Z |
| publishDate | 2021-12-01 |
| publisher | Editorial Office of Laboratory Animal and Comparative Medicine |
| record_format | Article |
| series | Shiyan dongwu yu bijiao yixue |
| spelling | doaj.art-c52ddedd8e8e4e9d8a1b197fc8dcef302022-12-22T03:44:05ZzhoEditorial Office of Laboratory Animal and Comparative MedicineShiyan dongwu yu bijiao yixue1674-58172021-12-0141654755310.12300/j.issn.1674-5817.2021.0191674-5817（2021）06-0547-07Research and Application Progress in Visualized RPA-LFD Nucleic Acid Detection TechnologyYU LingzhiTAO LingyunWEI XiaofengRecombinase polymerase amplification (RPA) is a novel isothermal nucleic acid amplification technology. Compared to PCR, it features ease of use, high efficiency, high sensitivity, high specificity, and does not need specific instruments, which allows it as an alternative to PCR and the most promising tool for rapid molecular diagnosis. RPA combined with lateral flow dipstick (LFD) (RPA-LFD) enables visual detection of amplified products and has promising applications for rapid nucleic acid detection of pathogens on site. The novel technique provides a new method for quality supervision and inspection of laboratory animals. In this paper, we reviewed the principle, research status, and technical difficulties of RPA-LFD, as well as the progress in rapid extraction of nucleic acid on site.https://www.slarc.org.cn/dwyx/CN/10.12300/j.issn.1674-5817.2021.019recombinase polymerase amplificationlateral flow dipstickrapid extraction of nucleic acid on sitesurveillance and control of animal epidemic diseases on site |
| spellingShingle | YU Lingzhi TAO Lingyun WEI Xiaofeng Research and Application Progress in Visualized RPA-LFD Nucleic Acid Detection Technology Shiyan dongwu yu bijiao yixue recombinase polymerase amplification lateral flow dipstick rapid extraction of nucleic acid on site surveillance and control of animal epidemic diseases on site |
| title | Research and Application Progress in Visualized RPA-LFD Nucleic Acid Detection Technology |
| title_full | Research and Application Progress in Visualized RPA-LFD Nucleic Acid Detection Technology |
| title_fullStr | Research and Application Progress in Visualized RPA-LFD Nucleic Acid Detection Technology |
| title_full_unstemmed | Research and Application Progress in Visualized RPA-LFD Nucleic Acid Detection Technology |
| title_short | Research and Application Progress in Visualized RPA-LFD Nucleic Acid Detection Technology |
| title_sort | research and application progress in visualized rpa lfd nucleic acid detection technology |
| topic | recombinase polymerase amplification lateral flow dipstick rapid extraction of nucleic acid on site surveillance and control of animal epidemic diseases on site |
| url | https://www.slarc.org.cn/dwyx/CN/10.12300/j.issn.1674-5817.2021.019 |
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