Berberine Suppresses TNF-α-induced MMP-9 and Cell Invasion through Inhibition of AP-1 Activity in MDA-MB-231 Human Breast Cancer Cells
Invasion of cancer cell induced by matrix metalloproteinase-9 (MMP-9) is one of pivotal steps in cancer metastasis. Herein, we investigated how cell invasion was regulated by berberine (BBR), an isoquinoline derivative alkaloid compound, in MDA-MB-231 human breast cancer cells. The basal level of MM...
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MDPI AG
2008-12-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/13/12/2975/ |
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| author | Jae Hyuck Choi Jong Bin Kim Seok Jin Nam Jung-Hyun Yang Jung-Han Kim Jeong Eon Lee Sangmin Kim |
| author_facet | Jae Hyuck Choi Jong Bin Kim Seok Jin Nam Jung-Hyun Yang Jung-Han Kim Jeong Eon Lee Sangmin Kim |
| author_sort | Jae Hyuck Choi |
| collection | DOAJ |
| description | Invasion of cancer cell induced by matrix metalloproteinase-9 (MMP-9) is one of pivotal steps in cancer metastasis. Herein, we investigated how cell invasion was regulated by berberine (BBR), an isoquinoline derivative alkaloid compound, in MDA-MB-231 human breast cancer cells. The basal level of MMP-9 activity and expression was dose-dependently increased by TNF-α, while TNF-a-induced MMP-9 gelatinase activity and expression was decreased by BBR. To investigate regulatory mechanism of TNF-α-induced MMP-9 expression, we pretreated cells with UO126 (MEK inhibitor), SB203580 (p38 inhibitor) and SP600125 (JNK inhibitor), respectively. Interestingly, TNF-α-induced MMP-9 activity and expression was decreased by UO126 and SB203580, but not by SP600125. Therefore, we further examined the effects of BBR on TNF-α-induced AP-1 DNA binding activity which is a downstream target of ERK and p38. Our data showed that TNF-α-induced AP-1 DNA binding activity was inhibited by BBR. Finally, we investigated the effect of BBR on TNF-α-induced cell invasion. TNF-α-induced cell invasion was significantly decreased by BBR treatment. Taken together, we suggest that TNF-α-induced MMP-9 expression and cell invasion are decreased by BBR through the suppression of AP-1 DNA binding activity in MDA-MB-231 human breast cancer cells. |
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| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-04-13T22:10:22Z |
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| spelling | doaj.art-c52fcffe955a40df9b1a0de242c54e512022-12-22T02:27:47ZengMDPI AGMolecules1420-30492008-12-0113122975298510.3390/molecules13122975Berberine Suppresses TNF-α-induced MMP-9 and Cell Invasion through Inhibition of AP-1 Activity in MDA-MB-231 Human Breast Cancer CellsJae Hyuck ChoiJong Bin KimSeok Jin NamJung-Hyun YangJung-Han KimJeong Eon LeeSangmin KimInvasion of cancer cell induced by matrix metalloproteinase-9 (MMP-9) is one of pivotal steps in cancer metastasis. Herein, we investigated how cell invasion was regulated by berberine (BBR), an isoquinoline derivative alkaloid compound, in MDA-MB-231 human breast cancer cells. The basal level of MMP-9 activity and expression was dose-dependently increased by TNF-α, while TNF-a-induced MMP-9 gelatinase activity and expression was decreased by BBR. To investigate regulatory mechanism of TNF-α-induced MMP-9 expression, we pretreated cells with UO126 (MEK inhibitor), SB203580 (p38 inhibitor) and SP600125 (JNK inhibitor), respectively. Interestingly, TNF-α-induced MMP-9 activity and expression was decreased by UO126 and SB203580, but not by SP600125. Therefore, we further examined the effects of BBR on TNF-α-induced AP-1 DNA binding activity which is a downstream target of ERK and p38. Our data showed that TNF-α-induced AP-1 DNA binding activity was inhibited by BBR. Finally, we investigated the effect of BBR on TNF-α-induced cell invasion. TNF-α-induced cell invasion was significantly decreased by BBR treatment. Taken together, we suggest that TNF-α-induced MMP-9 expression and cell invasion are decreased by BBR through the suppression of AP-1 DNA binding activity in MDA-MB-231 human breast cancer cells.http://www.mdpi.com/1420-3049/13/12/2975/BerberineAP-1MMP-9Cell invasion |
| spellingShingle | Jae Hyuck Choi Jong Bin Kim Seok Jin Nam Jung-Hyun Yang Jung-Han Kim Jeong Eon Lee Sangmin Kim Berberine Suppresses TNF-α-induced MMP-9 and Cell Invasion through Inhibition of AP-1 Activity in MDA-MB-231 Human Breast Cancer Cells Molecules Berberine AP-1 MMP-9 Cell invasion |
| title | Berberine Suppresses TNF-α-induced MMP-9 and Cell Invasion through Inhibition of AP-1 Activity in MDA-MB-231 Human Breast Cancer Cells |
| title_full | Berberine Suppresses TNF-α-induced MMP-9 and Cell Invasion through Inhibition of AP-1 Activity in MDA-MB-231 Human Breast Cancer Cells |
| title_fullStr | Berberine Suppresses TNF-α-induced MMP-9 and Cell Invasion through Inhibition of AP-1 Activity in MDA-MB-231 Human Breast Cancer Cells |
| title_full_unstemmed | Berberine Suppresses TNF-α-induced MMP-9 and Cell Invasion through Inhibition of AP-1 Activity in MDA-MB-231 Human Breast Cancer Cells |
| title_short | Berberine Suppresses TNF-α-induced MMP-9 and Cell Invasion through Inhibition of AP-1 Activity in MDA-MB-231 Human Breast Cancer Cells |
| title_sort | berberine suppresses tnf aza induced mmp 9 and cell invasion through inhibition of ap 1 activity in mda mb 231 human breast cancer cells |
| topic | Berberine AP-1 MMP-9 Cell invasion |
| url | http://www.mdpi.com/1420-3049/13/12/2975/ |
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