Regulation of RNA Interference Pathways in the Insect Vector <i>Laodelphax striatellus</i> by Viral Proteins of Rice Stripe Virus
RNA interference (RNAi), especially the small interfering RNA (siRNA) and microRNA (miRNA) pathways, plays an important role in defending against viruses in plants and insects. However, how insect-transmitted phytoviruses regulate the RNAi-mediated antiviral response in vector insects has barely bee...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-08-01
|
| Series: | Viruses |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1999-4915/13/8/1591 |
| _version_ | 1829454371398615040 |
|---|---|
| author | Yan Xiao Qiong Li Wei Wang Yumei Fu Feng Cui |
| author_facet | Yan Xiao Qiong Li Wei Wang Yumei Fu Feng Cui |
| author_sort | Yan Xiao |
| collection | DOAJ |
| description | RNA interference (RNAi), especially the small interfering RNA (siRNA) and microRNA (miRNA) pathways, plays an important role in defending against viruses in plants and insects. However, how insect-transmitted phytoviruses regulate the RNAi-mediated antiviral response in vector insects has barely been uncovered. In this study, we explored the interaction between rice stripe virus (RSV) and the miRNA and siRNA pathways of the small brown planthopper, which is a vector insect. The transcript and protein levels of key genes in the two RNAi pathways did not change during the RSV infection process. When the expression of insect <i>Ago1</i>, <i>Ago2</i>, or <i>Translin</i> was silenced by the injection of double-stranded RNAs targeting these genes, viral replication was promoted with <i>Ago2</i> silencing but inhibited with <i>Translin</i> silencing. Protein-protein binding assays showed that viral NS2 and RNA-dependent RNA polymerase interacted with insect Ago2 and Translin, respectively. When <i>NS2</i> was knocked down, the transcript level of <i>Ago2</i> increased and viral replication was inhibited. Therefore, viral NS2 behaved like an siRNA suppressor in vector insects. This protein-binding regulation of insect RNAi systems reflects a complicated and diverse coevolution of viruses with their vector insects. |
| first_indexed | 2024-03-10T08:17:56Z |
| format | Article |
| id | doaj.art-c530a413cbb3434d898a9964b1e322a6 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-10T08:17:56Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-c530a413cbb3434d898a9964b1e322a62023-11-22T10:11:46ZengMDPI AGViruses1999-49152021-08-01138159110.3390/v13081591Regulation of RNA Interference Pathways in the Insect Vector <i>Laodelphax striatellus</i> by Viral Proteins of Rice Stripe VirusYan Xiao0Qiong Li1Wei Wang2Yumei Fu3Feng Cui4State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, ChinaState Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, ChinaState Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, ChinaKey Laboratory of Tropical Translational Medicine of Ministry of Education, School of Tropical Medicine and Laboratory Medicine, Hainan Medical University, Haikou 571199, ChinaState Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, ChinaRNA interference (RNAi), especially the small interfering RNA (siRNA) and microRNA (miRNA) pathways, plays an important role in defending against viruses in plants and insects. However, how insect-transmitted phytoviruses regulate the RNAi-mediated antiviral response in vector insects has barely been uncovered. In this study, we explored the interaction between rice stripe virus (RSV) and the miRNA and siRNA pathways of the small brown planthopper, which is a vector insect. The transcript and protein levels of key genes in the two RNAi pathways did not change during the RSV infection process. When the expression of insect <i>Ago1</i>, <i>Ago2</i>, or <i>Translin</i> was silenced by the injection of double-stranded RNAs targeting these genes, viral replication was promoted with <i>Ago2</i> silencing but inhibited with <i>Translin</i> silencing. Protein-protein binding assays showed that viral NS2 and RNA-dependent RNA polymerase interacted with insect Ago2 and Translin, respectively. When <i>NS2</i> was knocked down, the transcript level of <i>Ago2</i> increased and viral replication was inhibited. Therefore, viral NS2 behaved like an siRNA suppressor in vector insects. This protein-binding regulation of insect RNAi systems reflects a complicated and diverse coevolution of viruses with their vector insects.https://www.mdpi.com/1999-4915/13/8/1591siRNAmiRNArice stripe virusplanthoppertranslin<i>Ago2</i> |
| spellingShingle | Yan Xiao Qiong Li Wei Wang Yumei Fu Feng Cui Regulation of RNA Interference Pathways in the Insect Vector <i>Laodelphax striatellus</i> by Viral Proteins of Rice Stripe Virus Viruses siRNA miRNA rice stripe virus planthopper translin <i>Ago2</i> |
| title | Regulation of RNA Interference Pathways in the Insect Vector <i>Laodelphax striatellus</i> by Viral Proteins of Rice Stripe Virus |
| title_full | Regulation of RNA Interference Pathways in the Insect Vector <i>Laodelphax striatellus</i> by Viral Proteins of Rice Stripe Virus |
| title_fullStr | Regulation of RNA Interference Pathways in the Insect Vector <i>Laodelphax striatellus</i> by Viral Proteins of Rice Stripe Virus |
| title_full_unstemmed | Regulation of RNA Interference Pathways in the Insect Vector <i>Laodelphax striatellus</i> by Viral Proteins of Rice Stripe Virus |
| title_short | Regulation of RNA Interference Pathways in the Insect Vector <i>Laodelphax striatellus</i> by Viral Proteins of Rice Stripe Virus |
| title_sort | regulation of rna interference pathways in the insect vector i laodelphax striatellus i by viral proteins of rice stripe virus |
| topic | siRNA miRNA rice stripe virus planthopper translin <i>Ago2</i> |
| url | https://www.mdpi.com/1999-4915/13/8/1591 |
| work_keys_str_mv | AT yanxiao regulationofrnainterferencepathwaysintheinsectvectorilaodelphaxstriatellusibyviralproteinsofricestripevirus AT qiongli regulationofrnainterferencepathwaysintheinsectvectorilaodelphaxstriatellusibyviralproteinsofricestripevirus AT weiwang regulationofrnainterferencepathwaysintheinsectvectorilaodelphaxstriatellusibyviralproteinsofricestripevirus AT yumeifu regulationofrnainterferencepathwaysintheinsectvectorilaodelphaxstriatellusibyviralproteinsofricestripevirus AT fengcui regulationofrnainterferencepathwaysintheinsectvectorilaodelphaxstriatellusibyviralproteinsofricestripevirus |