The severity of glomerular endothelial cell injury is associated with infiltrating macrophage heterogeneity in endocapillary proliferative glomerulonephritis
Abstract Endocapillary proliferation occurs in various types of glomerulonephritis (GN), with varying prognoses. We examined 42 renal biopsy samples representing endocapillary proliferative lesions from post-streptococcal acute GN (PSAGN), Henoch–Schönlein purpura nephritis (HSPN), and lupus nephrit...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2021-06-01
|
| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-92655-5 |
| _version_ | 1818460327565590528 |
|---|---|
| author | Momoko Arai Akiko Mii Tetsuya Kashiwagi Akira Shimizu Yukinao Sakai |
| author_facet | Momoko Arai Akiko Mii Tetsuya Kashiwagi Akira Shimizu Yukinao Sakai |
| author_sort | Momoko Arai |
| collection | DOAJ |
| description | Abstract Endocapillary proliferation occurs in various types of glomerulonephritis (GN), with varying prognoses. We examined 42 renal biopsy samples representing endocapillary proliferative lesions from post-streptococcal acute GN (PSAGN), Henoch–Schönlein purpura nephritis (HSPN), and lupus nephritis (LN). In PSAGN, the glomerular capillary network was maintained, although severe lesions displayed dots or short, curved lines, indicating CD34-positive capillaries and suggesting capillary obstruction. Conversely, patients with LN and HSPN displayed obstruction of CD34-positive capillaries with dissociation from the glomerular basement membrane even in mild lesions. According to computer-assisted morphologic analysis, the cell density did not differ between the diseases. However, in PSAGN, the number of capillary loops was significantly increased, with a larger glomerular capillary luminal area than in the other groups. In addition, the number and frequency of CD163-positive cells (M2 macrophages) tended to be higher in PSAGN, while there were no significant differences in the number of CD68-positive (total) macrophages. These results indicate that in PSAGN, endothelial cell damage is less severe, and angiogenesis may be promoted. The severity of endothelial cell injury in each disease may be associated with differences in infiltrating inflammatory cell phenotypes. |
| first_indexed | 2024-12-14T23:28:29Z |
| format | Article |
| id | doaj.art-c5311baf07594898b006d3ffba144134 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-14T23:28:29Z |
| publishDate | 2021-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-c5311baf07594898b006d3ffba1441342022-12-21T22:43:44ZengNature PortfolioScientific Reports2045-23222021-06-0111111110.1038/s41598-021-92655-5The severity of glomerular endothelial cell injury is associated with infiltrating macrophage heterogeneity in endocapillary proliferative glomerulonephritisMomoko Arai0Akiko Mii1Tetsuya Kashiwagi2Akira Shimizu3Yukinao Sakai4Department of Nephrology, Nippon Medical SchoolDepartment of Nephrology, Nippon Medical SchoolDepartment of Nephrology, Nippon Medical SchoolDepartment of Analytic Human Pathology, Nippon Medical SchoolDepartment of Nephrology, Nippon Medical SchoolAbstract Endocapillary proliferation occurs in various types of glomerulonephritis (GN), with varying prognoses. We examined 42 renal biopsy samples representing endocapillary proliferative lesions from post-streptococcal acute GN (PSAGN), Henoch–Schönlein purpura nephritis (HSPN), and lupus nephritis (LN). In PSAGN, the glomerular capillary network was maintained, although severe lesions displayed dots or short, curved lines, indicating CD34-positive capillaries and suggesting capillary obstruction. Conversely, patients with LN and HSPN displayed obstruction of CD34-positive capillaries with dissociation from the glomerular basement membrane even in mild lesions. According to computer-assisted morphologic analysis, the cell density did not differ between the diseases. However, in PSAGN, the number of capillary loops was significantly increased, with a larger glomerular capillary luminal area than in the other groups. In addition, the number and frequency of CD163-positive cells (M2 macrophages) tended to be higher in PSAGN, while there were no significant differences in the number of CD68-positive (total) macrophages. These results indicate that in PSAGN, endothelial cell damage is less severe, and angiogenesis may be promoted. The severity of endothelial cell injury in each disease may be associated with differences in infiltrating inflammatory cell phenotypes.https://doi.org/10.1038/s41598-021-92655-5 |
| spellingShingle | Momoko Arai Akiko Mii Tetsuya Kashiwagi Akira Shimizu Yukinao Sakai The severity of glomerular endothelial cell injury is associated with infiltrating macrophage heterogeneity in endocapillary proliferative glomerulonephritis Scientific Reports |
| title | The severity of glomerular endothelial cell injury is associated with infiltrating macrophage heterogeneity in endocapillary proliferative glomerulonephritis |
| title_full | The severity of glomerular endothelial cell injury is associated with infiltrating macrophage heterogeneity in endocapillary proliferative glomerulonephritis |
| title_fullStr | The severity of glomerular endothelial cell injury is associated with infiltrating macrophage heterogeneity in endocapillary proliferative glomerulonephritis |
| title_full_unstemmed | The severity of glomerular endothelial cell injury is associated with infiltrating macrophage heterogeneity in endocapillary proliferative glomerulonephritis |
| title_short | The severity of glomerular endothelial cell injury is associated with infiltrating macrophage heterogeneity in endocapillary proliferative glomerulonephritis |
| title_sort | severity of glomerular endothelial cell injury is associated with infiltrating macrophage heterogeneity in endocapillary proliferative glomerulonephritis |
| url | https://doi.org/10.1038/s41598-021-92655-5 |
| work_keys_str_mv | AT momokoarai theseverityofglomerularendothelialcellinjuryisassociatedwithinfiltratingmacrophageheterogeneityinendocapillaryproliferativeglomerulonephritis AT akikomii theseverityofglomerularendothelialcellinjuryisassociatedwithinfiltratingmacrophageheterogeneityinendocapillaryproliferativeglomerulonephritis AT tetsuyakashiwagi theseverityofglomerularendothelialcellinjuryisassociatedwithinfiltratingmacrophageheterogeneityinendocapillaryproliferativeglomerulonephritis AT akirashimizu theseverityofglomerularendothelialcellinjuryisassociatedwithinfiltratingmacrophageheterogeneityinendocapillaryproliferativeglomerulonephritis AT yukinaosakai theseverityofglomerularendothelialcellinjuryisassociatedwithinfiltratingmacrophageheterogeneityinendocapillaryproliferativeglomerulonephritis AT momokoarai severityofglomerularendothelialcellinjuryisassociatedwithinfiltratingmacrophageheterogeneityinendocapillaryproliferativeglomerulonephritis AT akikomii severityofglomerularendothelialcellinjuryisassociatedwithinfiltratingmacrophageheterogeneityinendocapillaryproliferativeglomerulonephritis AT tetsuyakashiwagi severityofglomerularendothelialcellinjuryisassociatedwithinfiltratingmacrophageheterogeneityinendocapillaryproliferativeglomerulonephritis AT akirashimizu severityofglomerularendothelialcellinjuryisassociatedwithinfiltratingmacrophageheterogeneityinendocapillaryproliferativeglomerulonephritis AT yukinaosakai severityofglomerularendothelialcellinjuryisassociatedwithinfiltratingmacrophageheterogeneityinendocapillaryproliferativeglomerulonephritis |