Chemical Characterization and Cytotoxic and Antioxidant Activity Evaluation of the Ethanol Extract from the Bulbs of <i>Pancratium maritimun</i> Collected in Sicily
<i>P. maritimum</i> L., belonging to the Amaryllidaceae family, is a species that grows on beaches and coastal sand dunes mainly on both sides of the Mediterranean Sea and Black Sea, the Middle East, and up to the Caucasus region. It has been largely investigated due to its several inter...
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MDPI AG
2023-05-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/28/10/3986 |
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| author | Adele Cicio Stefania Sut Stefano Dall’Acqua Maurizio Bruno Claudio Luparello Rosa Serio Maria Grazia Zizzo |
| author_facet | Adele Cicio Stefania Sut Stefano Dall’Acqua Maurizio Bruno Claudio Luparello Rosa Serio Maria Grazia Zizzo |
| author_sort | Adele Cicio |
| collection | DOAJ |
| description | <i>P. maritimum</i> L., belonging to the Amaryllidaceae family, is a species that grows on beaches and coastal sand dunes mainly on both sides of the Mediterranean Sea and Black Sea, the Middle East, and up to the Caucasus region. It has been largely investigated due to its several interesting biological properties. With the aim of providing new insights into the phytochemistry and pharmacology of this species, the ethanolic extract of the bulbs from a local accession, not previously studied, growing in Sicily (Italy), was investigated. This chemical analysis, performed by mono- and bi-dimensional NMR spectroscopy, as well as LC-DAD-MSn, allowed to identify several alkaloids, three of which were never detected in the genus <i>Pancratium.</i> Furthermore, the cytotoxicity of the preparation was assessed in differentiated human Caco-2 intestinal cells by trypan blue exclusion assay, and its antioxidant potential was evaluated using the DCFH-DA radical scavenging method. The results obtained demonstrate that <i>P. maritimum</i> bulbs’ extract exerts no cytotoxic effect and is able to remove free radicals at all the concentrations tested. |
| first_indexed | 2024-03-11T03:27:44Z |
| format | Article |
| id | doaj.art-c531f0912a654804bd2b9c6b3a477178 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-11T03:27:44Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-c531f0912a654804bd2b9c6b3a4771782023-11-18T02:37:37ZengMDPI AGMolecules1420-30492023-05-012810398610.3390/molecules28103986Chemical Characterization and Cytotoxic and Antioxidant Activity Evaluation of the Ethanol Extract from the Bulbs of <i>Pancratium maritimun</i> Collected in SicilyAdele Cicio0Stefania Sut1Stefano Dall’Acqua2Maurizio Bruno3Claudio Luparello4Rosa Serio5Maria Grazia Zizzo6Department of Biological, Chemical and Pharmaceutical Sciences and Technologies (STEBICEF), Università degli Studi di Palermo, Viale delle Scienze, ed. 16, 90128 Palermo, ItalyDepartment of Pharmaceutical and Pharmacological Sciences, University of Padova, via F. Marzolo 5, 35131 Padova, ItalyDepartment of Pharmaceutical and Pharmacological Sciences, University of Padova, via F. Marzolo 5, 35131 Padova, ItalyDepartment of Biological, Chemical and Pharmaceutical Sciences and Technologies (STEBICEF), Università degli Studi di Palermo, Viale delle Scienze, ed. 16, 90128 Palermo, ItalyDepartment of Biological, Chemical and Pharmaceutical Sciences and Technologies (STEBICEF), Università degli Studi di Palermo, Viale delle Scienze, ed. 16, 90128 Palermo, ItalyDepartment of Biological, Chemical and Pharmaceutical Sciences and Technologies (STEBICEF), Università degli Studi di Palermo, Viale delle Scienze, ed. 16, 90128 Palermo, ItalyDepartment of Biological, Chemical and Pharmaceutical Sciences and Technologies (STEBICEF), Università degli Studi di Palermo, Viale delle Scienze, ed. 16, 90128 Palermo, Italy<i>P. maritimum</i> L., belonging to the Amaryllidaceae family, is a species that grows on beaches and coastal sand dunes mainly on both sides of the Mediterranean Sea and Black Sea, the Middle East, and up to the Caucasus region. It has been largely investigated due to its several interesting biological properties. With the aim of providing new insights into the phytochemistry and pharmacology of this species, the ethanolic extract of the bulbs from a local accession, not previously studied, growing in Sicily (Italy), was investigated. This chemical analysis, performed by mono- and bi-dimensional NMR spectroscopy, as well as LC-DAD-MSn, allowed to identify several alkaloids, three of which were never detected in the genus <i>Pancratium.</i> Furthermore, the cytotoxicity of the preparation was assessed in differentiated human Caco-2 intestinal cells by trypan blue exclusion assay, and its antioxidant potential was evaluated using the DCFH-DA radical scavenging method. The results obtained demonstrate that <i>P. maritimum</i> bulbs’ extract exerts no cytotoxic effect and is able to remove free radicals at all the concentrations tested.https://www.mdpi.com/1420-3049/28/10/3986Amaryllidaceaealkaloidscytotoxic activityantioxidant activityCaco-2 cells |
| spellingShingle | Adele Cicio Stefania Sut Stefano Dall’Acqua Maurizio Bruno Claudio Luparello Rosa Serio Maria Grazia Zizzo Chemical Characterization and Cytotoxic and Antioxidant Activity Evaluation of the Ethanol Extract from the Bulbs of <i>Pancratium maritimun</i> Collected in Sicily Molecules Amaryllidaceae alkaloids cytotoxic activity antioxidant activity Caco-2 cells |
| title | Chemical Characterization and Cytotoxic and Antioxidant Activity Evaluation of the Ethanol Extract from the Bulbs of <i>Pancratium maritimun</i> Collected in Sicily |
| title_full | Chemical Characterization and Cytotoxic and Antioxidant Activity Evaluation of the Ethanol Extract from the Bulbs of <i>Pancratium maritimun</i> Collected in Sicily |
| title_fullStr | Chemical Characterization and Cytotoxic and Antioxidant Activity Evaluation of the Ethanol Extract from the Bulbs of <i>Pancratium maritimun</i> Collected in Sicily |
| title_full_unstemmed | Chemical Characterization and Cytotoxic and Antioxidant Activity Evaluation of the Ethanol Extract from the Bulbs of <i>Pancratium maritimun</i> Collected in Sicily |
| title_short | Chemical Characterization and Cytotoxic and Antioxidant Activity Evaluation of the Ethanol Extract from the Bulbs of <i>Pancratium maritimun</i> Collected in Sicily |
| title_sort | chemical characterization and cytotoxic and antioxidant activity evaluation of the ethanol extract from the bulbs of i pancratium maritimun i collected in sicily |
| topic | Amaryllidaceae alkaloids cytotoxic activity antioxidant activity Caco-2 cells |
| url | https://www.mdpi.com/1420-3049/28/10/3986 |
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