Comparison of high-performance thin layer chromatography/UV-densitometry and UV-derivative spectrophotometry for the determination of trimetazidine in pharmaceutical formulations
New methods for assaying trimetazidine dihydrochloride on the basis of thin layer chromatography and spectrophotometry are proposed and compared in the paper. In HPTLC/UV-densitometry, separation is achieved by using a mobile phase composed of ammonia-methanol (30:70, V/V) on silica gel HPTLC plates...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Sciendo
2019-09-01
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| Series: | Acta Pharmaceutica |
| Subjects: | |
| Online Access: | https://doi.org/10.2478/acph-2019-0028 |
| _version_ | 1818586818655813632 |
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| author | Gackowski Marcin Koba Marcin Mądra-Gackowska Katarzyna Kruszewski Stefan |
| author_facet | Gackowski Marcin Koba Marcin Mądra-Gackowska Katarzyna Kruszewski Stefan |
| author_sort | Gackowski Marcin |
| collection | DOAJ |
| description | New methods for assaying trimetazidine dihydrochloride on the basis of thin layer chromatography and spectrophotometry are proposed and compared in the paper. In HPTLC/UV-densitometry, separation is achieved by using a mobile phase composed of ammonia-methanol (30:70, V/V) on silica gel HPTLC plates F254. Quantification using a non-linear calibration curve is accomplished by densito-metric detection at 230 nm. Derivative spectrophotometric determination of trimetazidine dihydrochloride is carried out from the fourth derivative of the absorbance at 233 nm in peak-zero mode. Statistical comparison led to the conclusion that there is no significant difference between the two studied methods and, moreover, that they demonstrate satisfactory accuracy and precision for routine applications. |
| first_indexed | 2024-12-16T08:59:01Z |
| format | Article |
| id | doaj.art-c53373f599704aeda9461af651e40d23 |
| institution | Directory Open Access Journal |
| issn | 1846-9558 |
| language | English |
| last_indexed | 2024-12-16T08:59:01Z |
| publishDate | 2019-09-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Acta Pharmaceutica |
| spelling | doaj.art-c53373f599704aeda9461af651e40d232022-12-21T22:37:13ZengSciendoActa Pharmaceutica1846-95582019-09-0169341342210.2478/acph-2019-0028acph-2019-0028Comparison of high-performance thin layer chromatography/UV-densitometry and UV-derivative spectrophotometry for the determination of trimetazidine in pharmaceutical formulationsGackowski Marcin0Koba Marcin1Mądra-Gackowska Katarzyna2Kruszewski Stefan3Department of Toxicology, Faculty of Pharmacy, Collegium Medicum of Nicolaus Copernicus University, Bydgoszcz, PolandDepartment of Toxicology, Faculty of Pharmacy, Collegium Medicum of Nicolaus Copernicus University, Bydgoszcz, PolandDepartment of Geriatrics, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Toruń, 85-094Bydgoszcz, PolandMedical Physics Division, Biophysics Department, Faculty of Pharmacy Collegium Medicum, Nicolaus Copernicus University, Bydgoszcz, PolandNew methods for assaying trimetazidine dihydrochloride on the basis of thin layer chromatography and spectrophotometry are proposed and compared in the paper. In HPTLC/UV-densitometry, separation is achieved by using a mobile phase composed of ammonia-methanol (30:70, V/V) on silica gel HPTLC plates F254. Quantification using a non-linear calibration curve is accomplished by densito-metric detection at 230 nm. Derivative spectrophotometric determination of trimetazidine dihydrochloride is carried out from the fourth derivative of the absorbance at 233 nm in peak-zero mode. Statistical comparison led to the conclusion that there is no significant difference between the two studied methods and, moreover, that they demonstrate satisfactory accuracy and precision for routine applications.https://doi.org/10.2478/acph-2019-0028trimetazidine dihydrochloridehigh performance thin layer chromatography/uv-densitometryderivative spectrophotometrypharmaceutical formulations |
| spellingShingle | Gackowski Marcin Koba Marcin Mądra-Gackowska Katarzyna Kruszewski Stefan Comparison of high-performance thin layer chromatography/UV-densitometry and UV-derivative spectrophotometry for the determination of trimetazidine in pharmaceutical formulations Acta Pharmaceutica trimetazidine dihydrochloride high performance thin layer chromatography/uv-densitometry derivative spectrophotometry pharmaceutical formulations |
| title | Comparison of high-performance thin layer chromatography/UV-densitometry and UV-derivative spectrophotometry for the determination of trimetazidine in pharmaceutical formulations |
| title_full | Comparison of high-performance thin layer chromatography/UV-densitometry and UV-derivative spectrophotometry for the determination of trimetazidine in pharmaceutical formulations |
| title_fullStr | Comparison of high-performance thin layer chromatography/UV-densitometry and UV-derivative spectrophotometry for the determination of trimetazidine in pharmaceutical formulations |
| title_full_unstemmed | Comparison of high-performance thin layer chromatography/UV-densitometry and UV-derivative spectrophotometry for the determination of trimetazidine in pharmaceutical formulations |
| title_short | Comparison of high-performance thin layer chromatography/UV-densitometry and UV-derivative spectrophotometry for the determination of trimetazidine in pharmaceutical formulations |
| title_sort | comparison of high performance thin layer chromatography uv densitometry and uv derivative spectrophotometry for the determination of trimetazidine in pharmaceutical formulations |
| topic | trimetazidine dihydrochloride high performance thin layer chromatography/uv-densitometry derivative spectrophotometry pharmaceutical formulations |
| url | https://doi.org/10.2478/acph-2019-0028 |
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