The Glaciozyma antarctica genome reveals an array of systems that provide sustained responses towards temperature variations in a persistently cold habitat.
Extremely low temperatures present various challenges to life that include ice formation and effects on metabolic capacity. Psyhcrophilic microorganisms typically have an array of mechanisms to enable survival in cold temperatures. In this study, we sequenced and analysed the genome of a psychrophil...
| Main Authors: | , , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2018-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5791967?pdf=render |
| _version_ | 1818540682774577152 |
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| author | Mohd Firdaus-Raih Noor Haza Fazlin Hashim Izwan Bharudin Mohd Faizal Abu Bakar Kie Kyon Huang Halimah Alias Bernard K B Lee Mohd Noor Mat Isa Shuhaila Mat-Sharani Suhaila Sulaiman Lih Jinq Tay Radziah Zolkefli Yusuf Muhammad Noor Douglas Sie Nguong Law Siti Hamidah Abdul Rahman Rosli Md-Illias Farah Diba Abu Bakar Nazalan Najimudin Abdul Munir Abdul Murad Nor Muhammad Mahadi |
| author_facet | Mohd Firdaus-Raih Noor Haza Fazlin Hashim Izwan Bharudin Mohd Faizal Abu Bakar Kie Kyon Huang Halimah Alias Bernard K B Lee Mohd Noor Mat Isa Shuhaila Mat-Sharani Suhaila Sulaiman Lih Jinq Tay Radziah Zolkefli Yusuf Muhammad Noor Douglas Sie Nguong Law Siti Hamidah Abdul Rahman Rosli Md-Illias Farah Diba Abu Bakar Nazalan Najimudin Abdul Munir Abdul Murad Nor Muhammad Mahadi |
| author_sort | Mohd Firdaus-Raih |
| collection | DOAJ |
| description | Extremely low temperatures present various challenges to life that include ice formation and effects on metabolic capacity. Psyhcrophilic microorganisms typically have an array of mechanisms to enable survival in cold temperatures. In this study, we sequenced and analysed the genome of a psychrophilic yeast isolated in the Antarctic region, Glaciozyma antarctica. The genome annotation identified 7857 protein coding sequences. From the genome sequence analysis we were able to identify genes that encoded for proteins known to be associated with cold survival, in addition to annotating genes that are unique to G. antarctica. For genes that are known to be involved in cold adaptation such as anti-freeze proteins (AFPs), our gene expression analysis revealed that they were differentially transcribed over time and in response to different temperatures. This indicated the presence of an array of adaptation systems that can respond to a changing but persistent cold environment. We were also able to validate the activity of all the AFPs annotated where the recombinant AFPs demonstrated anti-freeze capacity. This work is an important foundation for further collective exploration into psychrophilic microbiology where among other potential, the genes unique to this species may represent a pool of novel mechanisms for cold survival. |
| first_indexed | 2024-12-11T21:58:35Z |
| format | Article |
| id | doaj.art-c535eb7e07ea42f887282ca670758a77 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-11T21:58:35Z |
| publishDate | 2018-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-c535eb7e07ea42f887282ca670758a772022-12-22T00:49:12ZengPublic Library of Science (PLoS)PLoS ONE1932-62032018-01-01131e018994710.1371/journal.pone.0189947The Glaciozyma antarctica genome reveals an array of systems that provide sustained responses towards temperature variations in a persistently cold habitat.Mohd Firdaus-RaihNoor Haza Fazlin HashimIzwan BharudinMohd Faizal Abu BakarKie Kyon HuangHalimah AliasBernard K B LeeMohd Noor Mat IsaShuhaila Mat-SharaniSuhaila SulaimanLih Jinq TayRadziah ZolkefliYusuf Muhammad NoorDouglas Sie Nguong LawSiti Hamidah Abdul RahmanRosli Md-IlliasFarah Diba Abu BakarNazalan NajimudinAbdul Munir Abdul MuradNor Muhammad MahadiExtremely low temperatures present various challenges to life that include ice formation and effects on metabolic capacity. Psyhcrophilic microorganisms typically have an array of mechanisms to enable survival in cold temperatures. In this study, we sequenced and analysed the genome of a psychrophilic yeast isolated in the Antarctic region, Glaciozyma antarctica. The genome annotation identified 7857 protein coding sequences. From the genome sequence analysis we were able to identify genes that encoded for proteins known to be associated with cold survival, in addition to annotating genes that are unique to G. antarctica. For genes that are known to be involved in cold adaptation such as anti-freeze proteins (AFPs), our gene expression analysis revealed that they were differentially transcribed over time and in response to different temperatures. This indicated the presence of an array of adaptation systems that can respond to a changing but persistent cold environment. We were also able to validate the activity of all the AFPs annotated where the recombinant AFPs demonstrated anti-freeze capacity. This work is an important foundation for further collective exploration into psychrophilic microbiology where among other potential, the genes unique to this species may represent a pool of novel mechanisms for cold survival.http://europepmc.org/articles/PMC5791967?pdf=render |
| spellingShingle | Mohd Firdaus-Raih Noor Haza Fazlin Hashim Izwan Bharudin Mohd Faizal Abu Bakar Kie Kyon Huang Halimah Alias Bernard K B Lee Mohd Noor Mat Isa Shuhaila Mat-Sharani Suhaila Sulaiman Lih Jinq Tay Radziah Zolkefli Yusuf Muhammad Noor Douglas Sie Nguong Law Siti Hamidah Abdul Rahman Rosli Md-Illias Farah Diba Abu Bakar Nazalan Najimudin Abdul Munir Abdul Murad Nor Muhammad Mahadi The Glaciozyma antarctica genome reveals an array of systems that provide sustained responses towards temperature variations in a persistently cold habitat. PLoS ONE |
| title | The Glaciozyma antarctica genome reveals an array of systems that provide sustained responses towards temperature variations in a persistently cold habitat. |
| title_full | The Glaciozyma antarctica genome reveals an array of systems that provide sustained responses towards temperature variations in a persistently cold habitat. |
| title_fullStr | The Glaciozyma antarctica genome reveals an array of systems that provide sustained responses towards temperature variations in a persistently cold habitat. |
| title_full_unstemmed | The Glaciozyma antarctica genome reveals an array of systems that provide sustained responses towards temperature variations in a persistently cold habitat. |
| title_short | The Glaciozyma antarctica genome reveals an array of systems that provide sustained responses towards temperature variations in a persistently cold habitat. |
| title_sort | glaciozyma antarctica genome reveals an array of systems that provide sustained responses towards temperature variations in a persistently cold habitat |
| url | http://europepmc.org/articles/PMC5791967?pdf=render |
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