A Convenient Fluorescence-Based Assay for the Detection of Sucrose Transport and the Introduction of a Sucrose Transporter from Potato into <i>Clostridium</i> Strains
A convenient and effective sucrose transport assay for <i>Clostridium</i> strains is needed. Traditional methods, such as <sup>14</sup>C-sucrose isotope labelling, use radioactive materials and are not convenient for many laboratories. Here, a sucrose transporter from potato...
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| Format: | Article |
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MDPI AG
2019-09-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/24/19/3495 |
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| author | Zhikai Zhang Lihua Lin Hongchi Tang Shaowei Zeng Yuan Guo Yutuo Wei Ribo Huang Hao Pang Liqin Du |
| author_facet | Zhikai Zhang Lihua Lin Hongchi Tang Shaowei Zeng Yuan Guo Yutuo Wei Ribo Huang Hao Pang Liqin Du |
| author_sort | Zhikai Zhang |
| collection | DOAJ |
| description | A convenient and effective sucrose transport assay for <i>Clostridium</i> strains is needed. Traditional methods, such as <sup>14</sup>C-sucrose isotope labelling, use radioactive materials and are not convenient for many laboratories. Here, a sucrose transporter from potato was introduced into <i>Clostridium</i>, and a fluorescence assay based on esculin was used for the analysis of sucrose transport in <i>Clostridium</i> strains. This showed that the heterologously expressed potato sucrose transporter is functional in <i>Clostridium</i>. Recombinant engineering of high-level sucrose transport would aid sucrose fermentation in <i>Clostridium</i> strains. The assay described herein provides an important technological platform for studying sucrose transporter function following heterologous expression in <i>Clostridium</i>. |
| first_indexed | 2024-12-10T21:56:55Z |
| format | Article |
| id | doaj.art-c537662e01324b3288baffdccb9bcf75 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-10T21:56:55Z |
| publishDate | 2019-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-c537662e01324b3288baffdccb9bcf752022-12-22T01:32:01ZengMDPI AGMolecules1420-30492019-09-012419349510.3390/molecules24193495molecules24193495A Convenient Fluorescence-Based Assay for the Detection of Sucrose Transport and the Introduction of a Sucrose Transporter from Potato into <i>Clostridium</i> StrainsZhikai Zhang0Lihua Lin1Hongchi Tang2Shaowei Zeng3Yuan Guo4Yutuo Wei5Ribo Huang6Hao Pang7Liqin Du8State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi Research Center for Microbial and Enzymatic Technology, College of Life Science and Technology, Guangxi University, Daxue Road No. 100, Nanning 530005, ChinaNational Engineering Research Center for Non-Food Biorefinery, State Key Laboratory of Non-Food Biomass and Enzyme Technology, Guangxi Key Laboratory of Bio-refinery, Guangxi Academy of Sciences, Daling Road No. 98, Nanning 530007, ChinaNational Engineering Research Center for Non-Food Biorefinery, State Key Laboratory of Non-Food Biomass and Enzyme Technology, Guangxi Key Laboratory of Bio-refinery, Guangxi Academy of Sciences, Daling Road No. 98, Nanning 530007, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi Research Center for Microbial and Enzymatic Technology, College of Life Science and Technology, Guangxi University, Daxue Road No. 100, Nanning 530005, ChinaNational Engineering Research Center for Non-Food Biorefinery, State Key Laboratory of Non-Food Biomass and Enzyme Technology, Guangxi Key Laboratory of Bio-refinery, Guangxi Academy of Sciences, Daling Road No. 98, Nanning 530007, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi Research Center for Microbial and Enzymatic Technology, College of Life Science and Technology, Guangxi University, Daxue Road No. 100, Nanning 530005, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi Research Center for Microbial and Enzymatic Technology, College of Life Science and Technology, Guangxi University, Daxue Road No. 100, Nanning 530005, ChinaNational Engineering Research Center for Non-Food Biorefinery, State Key Laboratory of Non-Food Biomass and Enzyme Technology, Guangxi Key Laboratory of Bio-refinery, Guangxi Academy of Sciences, Daling Road No. 98, Nanning 530007, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi Research Center for Microbial and Enzymatic Technology, College of Life Science and Technology, Guangxi University, Daxue Road No. 100, Nanning 530005, ChinaA convenient and effective sucrose transport assay for <i>Clostridium</i> strains is needed. Traditional methods, such as <sup>14</sup>C-sucrose isotope labelling, use radioactive materials and are not convenient for many laboratories. Here, a sucrose transporter from potato was introduced into <i>Clostridium</i>, and a fluorescence assay based on esculin was used for the analysis of sucrose transport in <i>Clostridium</i> strains. This showed that the heterologously expressed potato sucrose transporter is functional in <i>Clostridium</i>. Recombinant engineering of high-level sucrose transport would aid sucrose fermentation in <i>Clostridium</i> strains. The assay described herein provides an important technological platform for studying sucrose transporter function following heterologous expression in <i>Clostridium</i>.https://www.mdpi.com/1420-3049/24/19/3495sucrose transporteresculinfluorescence assay<i>clostridium</i> |
| spellingShingle | Zhikai Zhang Lihua Lin Hongchi Tang Shaowei Zeng Yuan Guo Yutuo Wei Ribo Huang Hao Pang Liqin Du A Convenient Fluorescence-Based Assay for the Detection of Sucrose Transport and the Introduction of a Sucrose Transporter from Potato into <i>Clostridium</i> Strains Molecules sucrose transporter esculin fluorescence assay <i>clostridium</i> |
| title | A Convenient Fluorescence-Based Assay for the Detection of Sucrose Transport and the Introduction of a Sucrose Transporter from Potato into <i>Clostridium</i> Strains |
| title_full | A Convenient Fluorescence-Based Assay for the Detection of Sucrose Transport and the Introduction of a Sucrose Transporter from Potato into <i>Clostridium</i> Strains |
| title_fullStr | A Convenient Fluorescence-Based Assay for the Detection of Sucrose Transport and the Introduction of a Sucrose Transporter from Potato into <i>Clostridium</i> Strains |
| title_full_unstemmed | A Convenient Fluorescence-Based Assay for the Detection of Sucrose Transport and the Introduction of a Sucrose Transporter from Potato into <i>Clostridium</i> Strains |
| title_short | A Convenient Fluorescence-Based Assay for the Detection of Sucrose Transport and the Introduction of a Sucrose Transporter from Potato into <i>Clostridium</i> Strains |
| title_sort | convenient fluorescence based assay for the detection of sucrose transport and the introduction of a sucrose transporter from potato into i clostridium i strains |
| topic | sucrose transporter esculin fluorescence assay <i>clostridium</i> |
| url | https://www.mdpi.com/1420-3049/24/19/3495 |
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