Synthesis, Characterization and Evaluation of Potential Antioxidant Free Radical Scavenging Activity of Novel Synthetic Flavonoid
Background: Natural products like flavonoids from the natural or synthetic origin are known to possess anticancer, antioxidant, anti-inflammatory activities. However, there is few/no experimental proof presently available regarding these. In the present study, hydroxy thiophene derivative of flavono...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wolters Kluwer Medknow Publications
2020-01-01
|
| Series: | BLDE University Journal of Health Sciences |
| Subjects: | |
| Online Access: | http://www.bldeujournalhs.in/article.asp?issn=2468-838X;year=2020;volume=5;issue=3;spage=50;epage=51;aulast=Bhixavatimath;type=0 |
| _version_ | 1818600759953981440 |
|---|---|
| author | Prabhulingayya S Bhixavatimath Yasmeen Maniyar Akram Naikwadi |
| author_facet | Prabhulingayya S Bhixavatimath Yasmeen Maniyar Akram Naikwadi |
| author_sort | Prabhulingayya S Bhixavatimath |
| collection | DOAJ |
| description | Background: Natural products like flavonoids from the natural or synthetic origin are known to possess anticancer, antioxidant, anti-inflammatory activities. However, there is few/no experimental proof presently available regarding these. In the present study, hydroxy thiophene derivative of flavonoid was synthesized by Claisen–Schmidt reaction using grinding technique and evaluated for antioxidant activity.
Objective: The present study was aimed to synthesize, characterize and evaluate the free radical scavenging antioxidant activity by using in-vitro assay methods.
Methods: Synthesized compound was characterized on the basis of Fourier-Transform Infrared Spectroscopy (FTIR), proton Nuclear Magnetic Resonance (1H NMR),13 C NMR, Mass Spectroscopy (MS) and elemental analysis. The in vitro scavenging activity was studied on hydroxyl, nitric oxide radicals and superoxide anions. Further in the in vivo studies, the liver homogenate of diabetic mellitus rats induced by low dose streptozotocin fed on a high-fat diet, estimated for lipid peroxidase (TBARS), catalase(CAT), superoxide dismutase(SOD) activity and reduced glutathione (GSH) content by colorimetric assays.
Results: The NF compound exhibited the dose-dependent free radical scavenging activity especially at 100mcg to 200mcg/ml concentration but lesser than that of standard drugs.
Conclusions: The present study was demonstrated that the novel flavonoid (NF) found to have high antioxidant free radical scavenging activity against various oxidative stress systems which needs to be confirmed by other assays. |
| first_indexed | 2024-12-16T12:40:36Z |
| format | Article |
| id | doaj.art-c53a652cf587446eae4a6eeea16db309 |
| institution | Directory Open Access Journal |
| issn | 2468-838X 2456-1975 |
| language | English |
| last_indexed | 2024-12-16T12:40:36Z |
| publishDate | 2020-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | BLDE University Journal of Health Sciences |
| spelling | doaj.art-c53a652cf587446eae4a6eeea16db3092022-12-21T22:31:26ZengWolters Kluwer Medknow PublicationsBLDE University Journal of Health Sciences2468-838X2456-19752020-01-0153505110.4103/2468-838X.303820Synthesis, Characterization and Evaluation of Potential Antioxidant Free Radical Scavenging Activity of Novel Synthetic FlavonoidPrabhulingayya S BhixavatimathYasmeen ManiyarAkram NaikwadiBackground: Natural products like flavonoids from the natural or synthetic origin are known to possess anticancer, antioxidant, anti-inflammatory activities. However, there is few/no experimental proof presently available regarding these. In the present study, hydroxy thiophene derivative of flavonoid was synthesized by Claisen–Schmidt reaction using grinding technique and evaluated for antioxidant activity. Objective: The present study was aimed to synthesize, characterize and evaluate the free radical scavenging antioxidant activity by using in-vitro assay methods. Methods: Synthesized compound was characterized on the basis of Fourier-Transform Infrared Spectroscopy (FTIR), proton Nuclear Magnetic Resonance (1H NMR),13 C NMR, Mass Spectroscopy (MS) and elemental analysis. The in vitro scavenging activity was studied on hydroxyl, nitric oxide radicals and superoxide anions. Further in the in vivo studies, the liver homogenate of diabetic mellitus rats induced by low dose streptozotocin fed on a high-fat diet, estimated for lipid peroxidase (TBARS), catalase(CAT), superoxide dismutase(SOD) activity and reduced glutathione (GSH) content by colorimetric assays. Results: The NF compound exhibited the dose-dependent free radical scavenging activity especially at 100mcg to 200mcg/ml concentration but lesser than that of standard drugs. Conclusions: The present study was demonstrated that the novel flavonoid (NF) found to have high antioxidant free radical scavenging activity against various oxidative stress systems which needs to be confirmed by other assays.http://www.bldeujournalhs.in/article.asp?issn=2468-838X;year=2020;volume=5;issue=3;spage=50;epage=51;aulast=Bhixavatimath;type=0claisen–schmidtthiophenespectroscopyftirnmrhydroxylscavenging |
| spellingShingle | Prabhulingayya S Bhixavatimath Yasmeen Maniyar Akram Naikwadi Synthesis, Characterization and Evaluation of Potential Antioxidant Free Radical Scavenging Activity of Novel Synthetic Flavonoid BLDE University Journal of Health Sciences claisen–schmidt thiophene spectroscopy ftir nmr hydroxyl scavenging |
| title | Synthesis, Characterization and Evaluation of Potential Antioxidant Free Radical Scavenging Activity of Novel Synthetic Flavonoid |
| title_full | Synthesis, Characterization and Evaluation of Potential Antioxidant Free Radical Scavenging Activity of Novel Synthetic Flavonoid |
| title_fullStr | Synthesis, Characterization and Evaluation of Potential Antioxidant Free Radical Scavenging Activity of Novel Synthetic Flavonoid |
| title_full_unstemmed | Synthesis, Characterization and Evaluation of Potential Antioxidant Free Radical Scavenging Activity of Novel Synthetic Flavonoid |
| title_short | Synthesis, Characterization and Evaluation of Potential Antioxidant Free Radical Scavenging Activity of Novel Synthetic Flavonoid |
| title_sort | synthesis characterization and evaluation of potential antioxidant free radical scavenging activity of novel synthetic flavonoid |
| topic | claisen–schmidt thiophene spectroscopy ftir nmr hydroxyl scavenging |
| url | http://www.bldeujournalhs.in/article.asp?issn=2468-838X;year=2020;volume=5;issue=3;spage=50;epage=51;aulast=Bhixavatimath;type=0 |
| work_keys_str_mv | AT prabhulingayyasbhixavatimath synthesischaracterizationandevaluationofpotentialantioxidantfreeradicalscavengingactivityofnovelsyntheticflavonoid AT yasmeenmaniyar synthesischaracterizationandevaluationofpotentialantioxidantfreeradicalscavengingactivityofnovelsyntheticflavonoid AT akramnaikwadi synthesischaracterizationandevaluationofpotentialantioxidantfreeradicalscavengingactivityofnovelsyntheticflavonoid |