Soil Microbiome Study Based on DNA Extraction: A Review
In recent years, many different methods that allow for the analysis of the biodiversity and structure of the community of microorganisms inhabiting the soil environment have emerged. Many of these approaches are based on molecular methods including the study of genetic biodiversity based on DNA and...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-12-01
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| Series: | Water |
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| Online Access: | https://www.mdpi.com/2073-4441/14/24/3999 |
| _version_ | 1797454927490973696 |
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| author | Urszula Wydro |
| author_facet | Urszula Wydro |
| author_sort | Urszula Wydro |
| collection | DOAJ |
| description | In recent years, many different methods that allow for the analysis of the biodiversity and structure of the community of microorganisms inhabiting the soil environment have emerged. Many of these approaches are based on molecular methods including the study of genetic biodiversity based on DNA and RNA analysis. They are superior to conventional methods because they do not rely on time-consuming laboratory in vitro cultures and biochemical analyses. Moreover, methods based on the analysis of genetic material are characterized by high sensitivity and repeatability. The abovementioned issues are the subject of many reviews. The novelty of this article is the summarization of the main aspects of soil biological research including genetic techniques, bioinformatics and statistical tools. This approach could be an introduction for scientists starting their work in the field of genetic soil analysis. Additionally, examples of the application of molecular methods in soil research are presented. |
| first_indexed | 2024-03-09T15:45:07Z |
| format | Article |
| id | doaj.art-c53cade2c6b949c0b901dfbfd078a7ab |
| institution | Directory Open Access Journal |
| issn | 2073-4441 |
| language | English |
| last_indexed | 2024-03-09T15:45:07Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Water |
| spelling | doaj.art-c53cade2c6b949c0b901dfbfd078a7ab2023-11-24T18:41:06ZengMDPI AGWater2073-44412022-12-011424399910.3390/w14243999Soil Microbiome Study Based on DNA Extraction: A ReviewUrszula Wydro0Department of Chemistry, Biology and Biotechnology, Faculty of Civil Engineering and Environmental Sciences, Bialystok University of Technology, 15-351 Bialystok, PolandIn recent years, many different methods that allow for the analysis of the biodiversity and structure of the community of microorganisms inhabiting the soil environment have emerged. Many of these approaches are based on molecular methods including the study of genetic biodiversity based on DNA and RNA analysis. They are superior to conventional methods because they do not rely on time-consuming laboratory in vitro cultures and biochemical analyses. Moreover, methods based on the analysis of genetic material are characterized by high sensitivity and repeatability. The abovementioned issues are the subject of many reviews. The novelty of this article is the summarization of the main aspects of soil biological research including genetic techniques, bioinformatics and statistical tools. This approach could be an introduction for scientists starting their work in the field of genetic soil analysis. Additionally, examples of the application of molecular methods in soil research are presented.https://www.mdpi.com/2073-4441/14/24/3999soil microbial diversityDNA extractionHTSbioinformaticsdata analysis |
| spellingShingle | Urszula Wydro Soil Microbiome Study Based on DNA Extraction: A Review Water soil microbial diversity DNA extraction HTS bioinformatics data analysis |
| title | Soil Microbiome Study Based on DNA Extraction: A Review |
| title_full | Soil Microbiome Study Based on DNA Extraction: A Review |
| title_fullStr | Soil Microbiome Study Based on DNA Extraction: A Review |
| title_full_unstemmed | Soil Microbiome Study Based on DNA Extraction: A Review |
| title_short | Soil Microbiome Study Based on DNA Extraction: A Review |
| title_sort | soil microbiome study based on dna extraction a review |
| topic | soil microbial diversity DNA extraction HTS bioinformatics data analysis |
| url | https://www.mdpi.com/2073-4441/14/24/3999 |
| work_keys_str_mv | AT urszulawydro soilmicrobiomestudybasedondnaextractionareview |