Phytochemical Screening, Cytotoxicity and Antioxidant Activities of Leaves Extracts from Eucalyptus citriodora
The genus Eucalyptus has been used in African traditional medicine for the treatment of cardiovascular diseases and diabetes. In this work, chloroform and methanol extracts from the leaves of Eucalyptus citriodora was investigated for their phytochemicals, cytotoxicity and antioxidant potentials. Ph...
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| Format: | Article |
| Language: | English |
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Mulawarman University
2021-06-01
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| Series: | Journal of Tropical Pharmacy and Chemistry |
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| Online Access: | https://jtpc.farmasi.unmul.ac.id/index.php/jtpc/article/view/280 |
| _version_ | 1797637479482785792 |
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| author | Musa Maikudi Idris Adamu Mohammed Yelwa Aminu Muhammad |
| author_facet | Musa Maikudi Idris Adamu Mohammed Yelwa Aminu Muhammad |
| author_sort | Musa Maikudi Idris |
| collection | DOAJ |
| description | The genus Eucalyptus has been used in African traditional medicine for the treatment of cardiovascular diseases and diabetes. In this work, chloroform and methanol extracts from the leaves of Eucalyptus citriodora was investigated for their phytochemicals, cytotoxicity and antioxidant potentials. Phytochemical screening of the extracts showed the presence of alkaloid, flavonoid, phenols, reducing sugar and steroids in the methanol extract. IR absorptions of the extract supported the presence of these phytochemicals by revealing the bands 3335 cm-1 (O-H), 1613, 1480 cm-1 (C=C) and 1721 cm-1 (C=O). Evaluation of the cytotoxicity of the methanol extract using brine shrimp assay, suggested that, the extract was not toxic with LC50 value of 1.64 mg/mL. Antioxidant potentials of both chloroform and methanol extracts were determined using phenolic content quantification and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging. Methanol extract (37.32 ?g TAE/mg) had higher phenolic content than the chloroform extract (12.09 ?g TAE/mg). The radical scavenging potentials of the extracts recorded inhibitory activities of IC50 values of 892.7 ?g/mL (chloroform extract) and 8.3 ?g/mL (methanol extracts) relative to the positive control values of 31.1 ?g/mL (butylated hydroxytoluene) and 3.5 ?g/mL (ascorbic acid). These results showed that E. citriodora might contain promising antioxidant agents. |
| first_indexed | 2024-03-11T12:49:01Z |
| format | Article |
| id | doaj.art-c53df3646198430caf64e8a38e838aa8 |
| institution | Directory Open Access Journal |
| issn | 2087-7099 2407-6090 |
| language | English |
| last_indexed | 2024-03-11T12:49:01Z |
| publishDate | 2021-06-01 |
| publisher | Mulawarman University |
| record_format | Article |
| series | Journal of Tropical Pharmacy and Chemistry |
| spelling | doaj.art-c53df3646198430caf64e8a38e838aa82023-11-04T06:00:44ZengMulawarman UniversityJournal of Tropical Pharmacy and Chemistry2087-70992407-60902021-06-015310.25026/jtpc.v5i3.280Phytochemical Screening, Cytotoxicity and Antioxidant Activities of Leaves Extracts from Eucalyptus citriodoraMusa Maikudi Idris0Adamu Mohammed Yelwa1Aminu Muhammad2Department of Pure and Industrial Chemistry, Faculty of Physical Sciences, Bayero University Kano, P.M.B. 3011, Kano, NigeriaDepartment of Chemistry, School of Science Education, Federal College of Education (Technical), Potiskum, Yobe State, NigeriaDepartment of Pure and Industrial Chemistry, Faculty of Physical Sciences, Bayero University, KanoThe genus Eucalyptus has been used in African traditional medicine for the treatment of cardiovascular diseases and diabetes. In this work, chloroform and methanol extracts from the leaves of Eucalyptus citriodora was investigated for their phytochemicals, cytotoxicity and antioxidant potentials. Phytochemical screening of the extracts showed the presence of alkaloid, flavonoid, phenols, reducing sugar and steroids in the methanol extract. IR absorptions of the extract supported the presence of these phytochemicals by revealing the bands 3335 cm-1 (O-H), 1613, 1480 cm-1 (C=C) and 1721 cm-1 (C=O). Evaluation of the cytotoxicity of the methanol extract using brine shrimp assay, suggested that, the extract was not toxic with LC50 value of 1.64 mg/mL. Antioxidant potentials of both chloroform and methanol extracts were determined using phenolic content quantification and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging. Methanol extract (37.32 ?g TAE/mg) had higher phenolic content than the chloroform extract (12.09 ?g TAE/mg). The radical scavenging potentials of the extracts recorded inhibitory activities of IC50 values of 892.7 ?g/mL (chloroform extract) and 8.3 ?g/mL (methanol extracts) relative to the positive control values of 31.1 ?g/mL (butylated hydroxytoluene) and 3.5 ?g/mL (ascorbic acid). These results showed that E. citriodora might contain promising antioxidant agents.https://jtpc.farmasi.unmul.ac.id/index.php/jtpc/article/view/280Eucalyptusphytochemicalsbrine shrimpantioxidant |
| spellingShingle | Musa Maikudi Idris Adamu Mohammed Yelwa Aminu Muhammad Phytochemical Screening, Cytotoxicity and Antioxidant Activities of Leaves Extracts from Eucalyptus citriodora Journal of Tropical Pharmacy and Chemistry Eucalyptus phytochemicals brine shrimp antioxidant |
| title | Phytochemical Screening, Cytotoxicity and Antioxidant Activities of Leaves Extracts from Eucalyptus citriodora |
| title_full | Phytochemical Screening, Cytotoxicity and Antioxidant Activities of Leaves Extracts from Eucalyptus citriodora |
| title_fullStr | Phytochemical Screening, Cytotoxicity and Antioxidant Activities of Leaves Extracts from Eucalyptus citriodora |
| title_full_unstemmed | Phytochemical Screening, Cytotoxicity and Antioxidant Activities of Leaves Extracts from Eucalyptus citriodora |
| title_short | Phytochemical Screening, Cytotoxicity and Antioxidant Activities of Leaves Extracts from Eucalyptus citriodora |
| title_sort | phytochemical screening cytotoxicity and antioxidant activities of leaves extracts from eucalyptus citriodora |
| topic | Eucalyptus phytochemicals brine shrimp antioxidant |
| url | https://jtpc.farmasi.unmul.ac.id/index.php/jtpc/article/view/280 |
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