Prevalence of silver resistance determinants and extended-spectrum β-lactamases in bacterial species causing wound infection: First report from Bangladesh
Background: The use of silver is rapidly rising in wound care and silver-containing dressings are widely used along with other antibiotics, particularly β-lactams. Consequently, concerns are being raised regarding the emergence of silver-resistance and cross-resistance to β-lactams. Therefore, this...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-03-01
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| Series: | New Microbes and New Infections |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2052297523000239 |
| _version_ | 1797844354127101952 |
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| author | Kazi Sarjana Safain Mohammad Sazzadul Islam Jumanah Amatullah Mohammad Al Mahmud-Un-Nabi Golam Sarower Bhuyan Jakia Rahman Suprovath Kumar Sarker Md Tarikul Islam Rosy Sultana Firdausi Qadri Kaiissar Mannoor |
| author_facet | Kazi Sarjana Safain Mohammad Sazzadul Islam Jumanah Amatullah Mohammad Al Mahmud-Un-Nabi Golam Sarower Bhuyan Jakia Rahman Suprovath Kumar Sarker Md Tarikul Islam Rosy Sultana Firdausi Qadri Kaiissar Mannoor |
| author_sort | Kazi Sarjana Safain |
| collection | DOAJ |
| description | Background: The use of silver is rapidly rising in wound care and silver-containing dressings are widely used along with other antibiotics, particularly β-lactams. Consequently, concerns are being raised regarding the emergence of silver-resistance and cross-resistance to β-lactams. Therefore, this study aimed to determine the phenotypic and genotypic profiles of silver-resistance and extended-spectrum β-lactamases in isolates from chronic wounds. Methods: 317 wound swab specimens were collected from tertiary hospitals of Dhaka city and analysed for the microbial identification. The antibiotic resistance/susceptibility profiles were determined and phenotypes of silver resistant isolates were examined. The presence of silver-resistance (sil) genes (silE, silP, and silS) and extended-spectrum β-lactamases (ESBL) (CTX-M-1, NDM-1, KPC, OXA-48, and VIM-1) were explored in isolated microorganisms. Results: A total of 501 strains were isolated with Staphylococcus aureus (24%) as the predominant organism. In 29% of the samples, polymicrobial infections were observed. A large proportion of Enterobacterales (59%) was resistant to carbapenems and a significantly high multiple antibiotic-resistance indexes (>0.2) were seen for 53% of organisms (P < 0.001). According to molecular analysis, the most prevalent types of ESBL and sil gene were CTX-M-1 (47%) and silE (42%), respectively. Furthermore, phenotypic silver-nitrate susceptibility testing showed significant minimum-inhibitory-concentration patterns between sil-negative and sil-positive isolates. We further observed co-occurrence of silver-resistance determinants and ESBLs (65%). Conclusions: Notably, this is the first-time detection of silver-resistance along with its co-detection with ESBLs in Bangladesh. This research highlights the need for selecting appropriate treatment strategies and developing new alternative therapies to minimize microbial infection in wounds. |
| first_indexed | 2024-04-09T17:20:55Z |
| format | Article |
| id | doaj.art-c53e03f27c8649d493e68dfa90da4318 |
| institution | Directory Open Access Journal |
| issn | 2052-2975 |
| language | English |
| last_indexed | 2024-04-09T17:20:55Z |
| publishDate | 2023-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | New Microbes and New Infections |
| spelling | doaj.art-c53e03f27c8649d493e68dfa90da43182023-04-19T04:22:27ZengElsevierNew Microbes and New Infections2052-29752023-03-0152101104Prevalence of silver resistance determinants and extended-spectrum β-lactamases in bacterial species causing wound infection: First report from BangladeshKazi Sarjana Safain0Mohammad Sazzadul Islam1Jumanah Amatullah2Mohammad Al Mahmud-Un-Nabi3Golam Sarower Bhuyan4Jakia Rahman5Suprovath Kumar Sarker6Md Tarikul Islam7Rosy Sultana8Firdausi Qadri9Kaiissar Mannoor10Infectious Diseases Laboratory, Institute for Developing Science and Health Initiatives, Dhaka, Bangladesh; Department of Mathematics and Natural Sciences, BRAC University, Dhaka, BangladeshGenetics and Genomics Laboratory, Institute for Developing Science and Health Initiatives, Dhaka, Bangladesh; Department of Biochemistry and Molecular Biology, Jagannath University, Dhaka, BangladeshInfectious Diseases Laboratory, Institute for Developing Science and Health Initiatives, Dhaka, Bangladesh; Department of Mathematics and Natural Sciences, BRAC University, Dhaka, BangladeshInfectious Diseases Laboratory, Institute for Developing Science and Health Initiatives, Dhaka, BangladeshInfectious Diseases Laboratory, Institute for Developing Science and Health Initiatives, Dhaka, BangladeshInfectious Diseases Laboratory, Institute for Developing Science and Health Initiatives, Dhaka, Bangladesh; Department of Mathematics and Natural Sciences, BRAC University, Dhaka, BangladeshGenetics and Genomics Laboratory, Institute for Developing Science and Health Initiatives, Dhaka, BangladeshGenetics and Genomics Laboratory, Institute for Developing Science and Health Initiatives, Dhaka, BangladeshInfectious Diseases Laboratory, Institute for Developing Science and Health Initiatives, Dhaka, Bangladesh; Department of Immunology, Bangladesh University of Health Sciences, Dhaka, BangladeshInfectious Diseases Laboratory, Institute for Developing Science and Health Initiatives, Dhaka, Bangladesh; Genetics and Genomics Laboratory, Institute for Developing Science and Health Initiatives, Dhaka, Bangladesh; Department of Enteric and Respiratory Infectious Diseases, Infectious Diseases Division, International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka, BangladeshInfectious Diseases Laboratory, Institute for Developing Science and Health Initiatives, Dhaka, Bangladesh; Genetics and Genomics Laboratory, Institute for Developing Science and Health Initiatives, Dhaka, Bangladesh; Corresponding author. Infectious Diseases Laboratory, Institute for Developing Science and Health Initiatives, Dhaka, BangladeshBackground: The use of silver is rapidly rising in wound care and silver-containing dressings are widely used along with other antibiotics, particularly β-lactams. Consequently, concerns are being raised regarding the emergence of silver-resistance and cross-resistance to β-lactams. Therefore, this study aimed to determine the phenotypic and genotypic profiles of silver-resistance and extended-spectrum β-lactamases in isolates from chronic wounds. Methods: 317 wound swab specimens were collected from tertiary hospitals of Dhaka city and analysed for the microbial identification. The antibiotic resistance/susceptibility profiles were determined and phenotypes of silver resistant isolates were examined. The presence of silver-resistance (sil) genes (silE, silP, and silS) and extended-spectrum β-lactamases (ESBL) (CTX-M-1, NDM-1, KPC, OXA-48, and VIM-1) were explored in isolated microorganisms. Results: A total of 501 strains were isolated with Staphylococcus aureus (24%) as the predominant organism. In 29% of the samples, polymicrobial infections were observed. A large proportion of Enterobacterales (59%) was resistant to carbapenems and a significantly high multiple antibiotic-resistance indexes (>0.2) were seen for 53% of organisms (P < 0.001). According to molecular analysis, the most prevalent types of ESBL and sil gene were CTX-M-1 (47%) and silE (42%), respectively. Furthermore, phenotypic silver-nitrate susceptibility testing showed significant minimum-inhibitory-concentration patterns between sil-negative and sil-positive isolates. We further observed co-occurrence of silver-resistance determinants and ESBLs (65%). Conclusions: Notably, this is the first-time detection of silver-resistance along with its co-detection with ESBLs in Bangladesh. This research highlights the need for selecting appropriate treatment strategies and developing new alternative therapies to minimize microbial infection in wounds.http://www.sciencedirect.com/science/article/pii/S2052297523000239Wound infectionSilver resistanceExtended-spectrum β-lactamaseCo-occurrenceLow-income countries |
| spellingShingle | Kazi Sarjana Safain Mohammad Sazzadul Islam Jumanah Amatullah Mohammad Al Mahmud-Un-Nabi Golam Sarower Bhuyan Jakia Rahman Suprovath Kumar Sarker Md Tarikul Islam Rosy Sultana Firdausi Qadri Kaiissar Mannoor Prevalence of silver resistance determinants and extended-spectrum β-lactamases in bacterial species causing wound infection: First report from Bangladesh New Microbes and New Infections Wound infection Silver resistance Extended-spectrum β-lactamase Co-occurrence Low-income countries |
| title | Prevalence of silver resistance determinants and extended-spectrum β-lactamases in bacterial species causing wound infection: First report from Bangladesh |
| title_full | Prevalence of silver resistance determinants and extended-spectrum β-lactamases in bacterial species causing wound infection: First report from Bangladesh |
| title_fullStr | Prevalence of silver resistance determinants and extended-spectrum β-lactamases in bacterial species causing wound infection: First report from Bangladesh |
| title_full_unstemmed | Prevalence of silver resistance determinants and extended-spectrum β-lactamases in bacterial species causing wound infection: First report from Bangladesh |
| title_short | Prevalence of silver resistance determinants and extended-spectrum β-lactamases in bacterial species causing wound infection: First report from Bangladesh |
| title_sort | prevalence of silver resistance determinants and extended spectrum β lactamases in bacterial species causing wound infection first report from bangladesh |
| topic | Wound infection Silver resistance Extended-spectrum β-lactamase Co-occurrence Low-income countries |
| url | http://www.sciencedirect.com/science/article/pii/S2052297523000239 |
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