Temperature synchronisation of circadian rhythms in primary human airway epithelial cells from children
Introduction Cellular circadian rhythms regulate immune pathways and inflammatory responses that mediate human disease such as asthma. Circadian rhythms in the lung may also contribute to exacerbations of chronic diseases such as asthma by regulating observed rhythms in mucus production, bronchial r...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMJ Publishing Group
2022-02-01
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| Series: | BMJ Open Respiratory Research |
| Online Access: | https://bmjopenrespres.bmj.com/content/9/1/e001319.full |
| _version_ | 1827899202522316800 |
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| author | Weston T Powell Lucille M Rich Elizabeth R Vanderwall Maria P White Jason S Debley |
| author_facet | Weston T Powell Lucille M Rich Elizabeth R Vanderwall Maria P White Jason S Debley |
| author_sort | Weston T Powell |
| collection | DOAJ |
| description | Introduction Cellular circadian rhythms regulate immune pathways and inflammatory responses that mediate human disease such as asthma. Circadian rhythms in the lung may also contribute to exacerbations of chronic diseases such as asthma by regulating observed rhythms in mucus production, bronchial reactivity, airway inflammation and airway resistance. Primary human airway epithelial cells (AECs) are commonly used to model human lung diseases, such as asthma, with circadian symptoms, but a method for synchronising circadian rhythms in AECs has not been developed, and the presence of circadian rhythms in human AECs remains uninvestigated.Methods We used temperature cycling to synchronise circadian rhythms in undifferentiated and differentiated primary human AECs. Reverse transcriptase-quantitative PCR was used to measure expression of the core circadian clock genes ARNTL, CLOCK, CRY1, CRY2, NR1D1, NR1D2, PER1 and PER2.Results Following temperature synchronisation, the core circadian genes ARNTL, CRY1, CRY2, NR1D1, NR1D2, PER1 and PER2 maintained endogenous 24-hour rhythms under constant conditions. Following serum shock, the core circadian genes ARNTL, NR1D1 and NR1D2 demonstrated rhythmic expression. Following temperature synchronisation, CXCL8 demonstrated rhythmic circadian expression.Conclusions Temperature synchronised circadian rhythms in AECs differentiated at an air–liquid interface can serve as a model to investigate circadian rhythms in pulmonary diseases. |
| first_indexed | 2024-03-12T23:14:48Z |
| format | Article |
| id | doaj.art-c53fff4510a8422d905772e53cf4f7e4 |
| institution | Directory Open Access Journal |
| issn | 2052-4439 |
| language | English |
| last_indexed | 2024-03-12T23:14:48Z |
| publishDate | 2022-02-01 |
| publisher | BMJ Publishing Group |
| record_format | Article |
| series | BMJ Open Respiratory Research |
| spelling | doaj.art-c53fff4510a8422d905772e53cf4f7e42023-07-17T14:30:07ZengBMJ Publishing GroupBMJ Open Respiratory Research2052-44392022-02-019110.1136/bmjresp-2022-001319Temperature synchronisation of circadian rhythms in primary human airway epithelial cells from childrenWeston T Powell0Lucille M Rich1Elizabeth R Vanderwall2Maria P White3Jason S Debley4Seattle Children`s Research Institute, Seattle, Washington, USASeattle Children`s Research Institute, Seattle, Washington, USASeattle Children`s Research Institute, Seattle, Washington, USASeattle Children`s Research Institute, Seattle, Washington, USASeattle Children`s Research Institute, Seattle, Washington, USAIntroduction Cellular circadian rhythms regulate immune pathways and inflammatory responses that mediate human disease such as asthma. Circadian rhythms in the lung may also contribute to exacerbations of chronic diseases such as asthma by regulating observed rhythms in mucus production, bronchial reactivity, airway inflammation and airway resistance. Primary human airway epithelial cells (AECs) are commonly used to model human lung diseases, such as asthma, with circadian symptoms, but a method for synchronising circadian rhythms in AECs has not been developed, and the presence of circadian rhythms in human AECs remains uninvestigated.Methods We used temperature cycling to synchronise circadian rhythms in undifferentiated and differentiated primary human AECs. Reverse transcriptase-quantitative PCR was used to measure expression of the core circadian clock genes ARNTL, CLOCK, CRY1, CRY2, NR1D1, NR1D2, PER1 and PER2.Results Following temperature synchronisation, the core circadian genes ARNTL, CRY1, CRY2, NR1D1, NR1D2, PER1 and PER2 maintained endogenous 24-hour rhythms under constant conditions. Following serum shock, the core circadian genes ARNTL, NR1D1 and NR1D2 demonstrated rhythmic expression. Following temperature synchronisation, CXCL8 demonstrated rhythmic circadian expression.Conclusions Temperature synchronised circadian rhythms in AECs differentiated at an air–liquid interface can serve as a model to investigate circadian rhythms in pulmonary diseases.https://bmjopenrespres.bmj.com/content/9/1/e001319.full |
| spellingShingle | Weston T Powell Lucille M Rich Elizabeth R Vanderwall Maria P White Jason S Debley Temperature synchronisation of circadian rhythms in primary human airway epithelial cells from children BMJ Open Respiratory Research |
| title | Temperature synchronisation of circadian rhythms in primary human airway epithelial cells from children |
| title_full | Temperature synchronisation of circadian rhythms in primary human airway epithelial cells from children |
| title_fullStr | Temperature synchronisation of circadian rhythms in primary human airway epithelial cells from children |
| title_full_unstemmed | Temperature synchronisation of circadian rhythms in primary human airway epithelial cells from children |
| title_short | Temperature synchronisation of circadian rhythms in primary human airway epithelial cells from children |
| title_sort | temperature synchronisation of circadian rhythms in primary human airway epithelial cells from children |
| url | https://bmjopenrespres.bmj.com/content/9/1/e001319.full |
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