Characterization of Secondary Metabolites of Leaf Buds from Some Species and Hybrids of <i>Populus</i> by Gas Chromatography Coupled with Mass Detection and Two-Dimensional High-Performance Thin-Layer Chromatography Methods with Assessment of Their Antioxidant Activity
Poplars provide medicinal raw plant materials used in pharmacy. Leaf buds are one of the herbal medicinal products collected from poplars, having anti-inflammatory and antiseptic properties, but there are no quality standards for their production and there is a need to determine their botanical sour...
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| Format: | Article |
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MDPI AG
2024-04-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/25/7/3971 |
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| author | Loretta Pobłocka-Olech Valery A. Isidorov Mirosława Krauze-Baranowska |
| author_facet | Loretta Pobłocka-Olech Valery A. Isidorov Mirosława Krauze-Baranowska |
| author_sort | Loretta Pobłocka-Olech |
| collection | DOAJ |
| description | Poplars provide medicinal raw plant materials used in pharmacy. Leaf buds are one of the herbal medicinal products collected from poplars, having anti-inflammatory and antiseptic properties, but there are no quality standards for their production and there is a need to determine their botanical sources. Therefore, the chemical compositions of the leaf buds from four species and varieties of poplars, <i>Populus balsamifera</i>, <i>P. × berolinensis</i>, <i>P. × canadensis</i> ‘Marilandica’, and <i>P. wilsonii</i> were investigated and compared using gas chromatography coupled with mass detection (GC-MS) and two-dimensional high-performance thin-layer chromatography (2D-HPTLC) in order to search for taxa characterized by a high content of biologically active compounds and with a diverse chemical composition that determines their therapeutic effects. The presence of 163 compounds belonging to the groups of flavonoids, phenolic acids derivatives, glycerides, and sesquiterpenes was revealed. Moreover, the conditions for the separation and identification of biologically active compounds occurring in analyzed leaf buds using 2D-HPTLC were optimized and used for metabolomic profiling of the studied poplars, enabling their fast and simple botanical identification. The total phenolic (TPC) and flavonoid (TFC) contents of examined extracts were determined and their antioxidant capacities were estimated by spectrophotometric DPPH, ABTS, and FRAP assays. Based on the analysis of phytochemicals and antioxidant activity, <i>P. × berolinensis</i> buds were selected as the raw plant material for medicinal purposes with the highest content of active compounds and the strongest antioxidant activity. |
| first_indexed | 2024-04-24T10:42:38Z |
| format | Article |
| id | doaj.art-c54012575a0c419882703b9c53245490 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-04-24T10:42:38Z |
| publishDate | 2024-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-c54012575a0c419882703b9c532454902024-04-12T13:20:16ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672024-04-01257397110.3390/ijms25073971Characterization of Secondary Metabolites of Leaf Buds from Some Species and Hybrids of <i>Populus</i> by Gas Chromatography Coupled with Mass Detection and Two-Dimensional High-Performance Thin-Layer Chromatography Methods with Assessment of Their Antioxidant ActivityLoretta Pobłocka-Olech0Valery A. Isidorov1Mirosława Krauze-Baranowska2Department of Pharmacognosy with Medicinal Plants Garden, Medical University of Gdańsk, 80-416 Gdańsk, PolandInstitute of Forest Sciences, Bialystok Technical University, 15-351 Bialystok, PolandDepartment of Pharmacognosy with Medicinal Plants Garden, Medical University of Gdańsk, 80-416 Gdańsk, PolandPoplars provide medicinal raw plant materials used in pharmacy. Leaf buds are one of the herbal medicinal products collected from poplars, having anti-inflammatory and antiseptic properties, but there are no quality standards for their production and there is a need to determine their botanical sources. Therefore, the chemical compositions of the leaf buds from four species and varieties of poplars, <i>Populus balsamifera</i>, <i>P. × berolinensis</i>, <i>P. × canadensis</i> ‘Marilandica’, and <i>P. wilsonii</i> were investigated and compared using gas chromatography coupled with mass detection (GC-MS) and two-dimensional high-performance thin-layer chromatography (2D-HPTLC) in order to search for taxa characterized by a high content of biologically active compounds and with a diverse chemical composition that determines their therapeutic effects. The presence of 163 compounds belonging to the groups of flavonoids, phenolic acids derivatives, glycerides, and sesquiterpenes was revealed. Moreover, the conditions for the separation and identification of biologically active compounds occurring in analyzed leaf buds using 2D-HPTLC were optimized and used for metabolomic profiling of the studied poplars, enabling their fast and simple botanical identification. The total phenolic (TPC) and flavonoid (TFC) contents of examined extracts were determined and their antioxidant capacities were estimated by spectrophotometric DPPH, ABTS, and FRAP assays. Based on the analysis of phytochemicals and antioxidant activity, <i>P. × berolinensis</i> buds were selected as the raw plant material for medicinal purposes with the highest content of active compounds and the strongest antioxidant activity.https://www.mdpi.com/1422-0067/25/7/3971poplar budsbioautographyflavonoidsphenolicsstandardization |
| spellingShingle | Loretta Pobłocka-Olech Valery A. Isidorov Mirosława Krauze-Baranowska Characterization of Secondary Metabolites of Leaf Buds from Some Species and Hybrids of <i>Populus</i> by Gas Chromatography Coupled with Mass Detection and Two-Dimensional High-Performance Thin-Layer Chromatography Methods with Assessment of Their Antioxidant Activity International Journal of Molecular Sciences poplar buds bioautography flavonoids phenolics standardization |
| title | Characterization of Secondary Metabolites of Leaf Buds from Some Species and Hybrids of <i>Populus</i> by Gas Chromatography Coupled with Mass Detection and Two-Dimensional High-Performance Thin-Layer Chromatography Methods with Assessment of Their Antioxidant Activity |
| title_full | Characterization of Secondary Metabolites of Leaf Buds from Some Species and Hybrids of <i>Populus</i> by Gas Chromatography Coupled with Mass Detection and Two-Dimensional High-Performance Thin-Layer Chromatography Methods with Assessment of Their Antioxidant Activity |
| title_fullStr | Characterization of Secondary Metabolites of Leaf Buds from Some Species and Hybrids of <i>Populus</i> by Gas Chromatography Coupled with Mass Detection and Two-Dimensional High-Performance Thin-Layer Chromatography Methods with Assessment of Their Antioxidant Activity |
| title_full_unstemmed | Characterization of Secondary Metabolites of Leaf Buds from Some Species and Hybrids of <i>Populus</i> by Gas Chromatography Coupled with Mass Detection and Two-Dimensional High-Performance Thin-Layer Chromatography Methods with Assessment of Their Antioxidant Activity |
| title_short | Characterization of Secondary Metabolites of Leaf Buds from Some Species and Hybrids of <i>Populus</i> by Gas Chromatography Coupled with Mass Detection and Two-Dimensional High-Performance Thin-Layer Chromatography Methods with Assessment of Their Antioxidant Activity |
| title_sort | characterization of secondary metabolites of leaf buds from some species and hybrids of i populus i by gas chromatography coupled with mass detection and two dimensional high performance thin layer chromatography methods with assessment of their antioxidant activity |
| topic | poplar buds bioautography flavonoids phenolics standardization |
| url | https://www.mdpi.com/1422-0067/25/7/3971 |
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