Taurine improves the wound healing process in cutaneous leishmaniasis in mice model, based on stereological parameters
Background: Cutaneous Leishmaniasis is a self-limiting disease caused by protozoan parasites of the genus Leishmania, which affects the skin with full-thickness wounds, which are prone to scar formation even after treatment. Taurine (Tu) is one of the most abundant amino acids that has antioxidant a...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2014-01-01
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| Series: | Advanced Biomedical Research |
| Subjects: | |
| Online Access: | http://www.advbiores.net/article.asp?issn=2277-9175;year=2014;volume=3;issue=1;spage=204;epage=204;aulast=Ashkani-Esfahani |
| _version_ | 1818959243390222336 |
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| author | Soheil Ashkani-Esfahani Fariba Zarifi Qasem Asgari Asal Zargari Samadnejad Shima Rafiee Ali Noorafshan |
| author_facet | Soheil Ashkani-Esfahani Fariba Zarifi Qasem Asgari Asal Zargari Samadnejad Shima Rafiee Ali Noorafshan |
| author_sort | Soheil Ashkani-Esfahani |
| collection | DOAJ |
| description | Background: Cutaneous Leishmaniasis is a self-limiting disease caused by protozoan parasites of the genus Leishmania, which affects the skin with full-thickness wounds, which are prone to scar formation even after treatment. Taurine (Tu) is one of the most abundant amino acids that has antioxidant and anti-inflammatory effects, which play an important role in the process of wound healing. Herein, we have investigated the effects of Tu on cutaneous Leishmaniasis wounds and L. major promastigotes.
Materials and Methods: Eighteen mice were induced with Leishmaniasis wounds (with L. Major) on the base of their tails and divided into three groups, T1: Treated with Tu injection, T2: Treated with Tu gel, and C: No treatment. Treatments were carried out every 24 hours for 21 days. The volume densities of the collagen bundles and vessels, vessel′s length density and diameter, and fibroblast populations were estimated by stereological methods. Flow cytometry was used in order to investigate the direct Tu effect on parasites. The Mann-Whitney U test was used and P ≤ 0.05 was considered to be statistically significant.
Results: The numerical density of the fibroblasts, volume density of the collagen bundles, and length densities of the vessels in groups T1 and T2 were significantly higher than in group C (P < 0.05). The fibroblast numerical density of group T1 was higher than that of group T2 (P = 0.02). Incidentally, Tu had no direct effect on L. major parasites according to the flow cytometry analysis.
Conclusion: Tu showed the ability to improve the wound healing process and tissue regeneration although it had no direct anti-leishmaniasis effect. |
| first_indexed | 2024-12-20T11:38:32Z |
| format | Article |
| id | doaj.art-c545b54a550d4fe795ddd517933e94c1 |
| institution | Directory Open Access Journal |
| issn | 2277-9175 2277-9175 |
| language | English |
| last_indexed | 2024-12-20T11:38:32Z |
| publishDate | 2014-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Advanced Biomedical Research |
| spelling | doaj.art-c545b54a550d4fe795ddd517933e94c12022-12-21T19:42:03ZengWolters Kluwer Medknow PublicationsAdvanced Biomedical Research2277-91752277-91752014-01-013120420410.4103/2277-9175.142314Taurine improves the wound healing process in cutaneous leishmaniasis in mice model, based on stereological parametersSoheil Ashkani-EsfahaniFariba ZarifiQasem AsgariAsal Zargari SamadnejadShima RafieeAli NoorafshanBackground: Cutaneous Leishmaniasis is a self-limiting disease caused by protozoan parasites of the genus Leishmania, which affects the skin with full-thickness wounds, which are prone to scar formation even after treatment. Taurine (Tu) is one of the most abundant amino acids that has antioxidant and anti-inflammatory effects, which play an important role in the process of wound healing. Herein, we have investigated the effects of Tu on cutaneous Leishmaniasis wounds and L. major promastigotes. Materials and Methods: Eighteen mice were induced with Leishmaniasis wounds (with L. Major) on the base of their tails and divided into three groups, T1: Treated with Tu injection, T2: Treated with Tu gel, and C: No treatment. Treatments were carried out every 24 hours for 21 days. The volume densities of the collagen bundles and vessels, vessel′s length density and diameter, and fibroblast populations were estimated by stereological methods. Flow cytometry was used in order to investigate the direct Tu effect on parasites. The Mann-Whitney U test was used and P ≤ 0.05 was considered to be statistically significant. Results: The numerical density of the fibroblasts, volume density of the collagen bundles, and length densities of the vessels in groups T1 and T2 were significantly higher than in group C (P < 0.05). The fibroblast numerical density of group T1 was higher than that of group T2 (P = 0.02). Incidentally, Tu had no direct effect on L. major parasites according to the flow cytometry analysis. Conclusion: Tu showed the ability to improve the wound healing process and tissue regeneration although it had no direct anti-leishmaniasis effect.http://www.advbiores.net/article.asp?issn=2277-9175;year=2014;volume=3;issue=1;spage=204;epage=204;aulast=Ashkani-EsfahaniCutaneous leishmaniasismicestereologytaurinewound healing |
| spellingShingle | Soheil Ashkani-Esfahani Fariba Zarifi Qasem Asgari Asal Zargari Samadnejad Shima Rafiee Ali Noorafshan Taurine improves the wound healing process in cutaneous leishmaniasis in mice model, based on stereological parameters Advanced Biomedical Research Cutaneous leishmaniasis mice stereology taurine wound healing |
| title | Taurine improves the wound healing process in cutaneous leishmaniasis in mice model, based on stereological parameters |
| title_full | Taurine improves the wound healing process in cutaneous leishmaniasis in mice model, based on stereological parameters |
| title_fullStr | Taurine improves the wound healing process in cutaneous leishmaniasis in mice model, based on stereological parameters |
| title_full_unstemmed | Taurine improves the wound healing process in cutaneous leishmaniasis in mice model, based on stereological parameters |
| title_short | Taurine improves the wound healing process in cutaneous leishmaniasis in mice model, based on stereological parameters |
| title_sort | taurine improves the wound healing process in cutaneous leishmaniasis in mice model based on stereological parameters |
| topic | Cutaneous leishmaniasis mice stereology taurine wound healing |
| url | http://www.advbiores.net/article.asp?issn=2277-9175;year=2014;volume=3;issue=1;spage=204;epage=204;aulast=Ashkani-Esfahani |
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