Enhanced bone regeneration by low-intensity pulsed ultrasound and lipid microbubbles on PLGA/TCP 3D-printed scaffolds
Abstract Background To investigate the effect of low-intensity pulsed ultrasound (LIPUS) combined with lipid microbubbles on the proliferation and bone regeneration of bone marrow mesenchymal stem cells (BMSCs) in poly (lactic-glycolic acid copolymer) (PLGA)/α-tricalcium phosphate (TCP) 3D-printed s...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-06-01
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| Series: | BMC Biotechnology |
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| Online Access: | https://doi.org/10.1186/s12896-023-00783-9 |
| _version_ | 1797806670539128832 |
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| author | Lin Jin Jiali Shan Yanhong Hao Yingchun Wang Liping Liu |
| author_facet | Lin Jin Jiali Shan Yanhong Hao Yingchun Wang Liping Liu |
| author_sort | Lin Jin |
| collection | DOAJ |
| description | Abstract Background To investigate the effect of low-intensity pulsed ultrasound (LIPUS) combined with lipid microbubbles on the proliferation and bone regeneration of bone marrow mesenchymal stem cells (BMSCs) in poly (lactic-glycolic acid copolymer) (PLGA)/α-tricalcium phosphate (TCP) 3D-printed scaffolds. Methods BMSCs were irradiated with different LIPUS parameters and microbubble concentrations, and the best acoustic excitation parameters were selected. The expression of type I collagen and the activity of alkaline phosphatase were detected. Alizarin red staining was used to evaluate the calcium salt production during osteogenic differentiation. Results BMSCs proliferation was the most significant under the condition of 0.5% (v/v) lipid microbubble concentration, 2.0 MHz frequency, 0.3 W/cm2 sound intensity and 20% duty cycle. After 14 days, the type I collagen expression and alkaline phosphatase activity in the scaffold increased significantly compared to those in the control group, and alizarin red staining showed more calcium salt production during osteogenic differentiation. After 21 days, scanning electron microscopy experiments showed that osteogenesis was obvious in the PLGA/TCP scaffolds. Conclusion LIPUS combined with lipid microbubbles on PLGA/TCP scaffolds can promote BMSCs growth and bone differentiation, which is expected to provide a new and effective method for the treatment of bone regeneration in tissue engineering. |
| first_indexed | 2024-03-13T06:10:48Z |
| format | Article |
| id | doaj.art-c54a93925bdb46b993d39b18273f98e4 |
| institution | Directory Open Access Journal |
| issn | 1472-6750 |
| language | English |
| last_indexed | 2024-03-13T06:10:48Z |
| publishDate | 2023-06-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Biotechnology |
| spelling | doaj.art-c54a93925bdb46b993d39b18273f98e42023-06-11T11:17:42ZengBMCBMC Biotechnology1472-67502023-06-012311810.1186/s12896-023-00783-9Enhanced bone regeneration by low-intensity pulsed ultrasound and lipid microbubbles on PLGA/TCP 3D-printed scaffoldsLin Jin0Jiali Shan1Yanhong Hao2Yingchun Wang3Liping Liu4Department of Ultrasound, Jiading District Central Hospital Affiliated Shanghai University of Medicine & Health SciencesDepartment of Ultrasound, Jiading District Central Hospital Affiliated Shanghai University of Medicine & Health SciencesDepartment of Ultrasound, First Hospital of Shanxi Medical UniversityDepartment of Ultrasound, Jiading District Central Hospital Affiliated Shanghai University of Medicine & Health SciencesDepartment of Ultrasound, First Hospital of Shanxi Medical UniversityAbstract Background To investigate the effect of low-intensity pulsed ultrasound (LIPUS) combined with lipid microbubbles on the proliferation and bone regeneration of bone marrow mesenchymal stem cells (BMSCs) in poly (lactic-glycolic acid copolymer) (PLGA)/α-tricalcium phosphate (TCP) 3D-printed scaffolds. Methods BMSCs were irradiated with different LIPUS parameters and microbubble concentrations, and the best acoustic excitation parameters were selected. The expression of type I collagen and the activity of alkaline phosphatase were detected. Alizarin red staining was used to evaluate the calcium salt production during osteogenic differentiation. Results BMSCs proliferation was the most significant under the condition of 0.5% (v/v) lipid microbubble concentration, 2.0 MHz frequency, 0.3 W/cm2 sound intensity and 20% duty cycle. After 14 days, the type I collagen expression and alkaline phosphatase activity in the scaffold increased significantly compared to those in the control group, and alizarin red staining showed more calcium salt production during osteogenic differentiation. After 21 days, scanning electron microscopy experiments showed that osteogenesis was obvious in the PLGA/TCP scaffolds. Conclusion LIPUS combined with lipid microbubbles on PLGA/TCP scaffolds can promote BMSCs growth and bone differentiation, which is expected to provide a new and effective method for the treatment of bone regeneration in tissue engineering.https://doi.org/10.1186/s12896-023-00783-9Bone repairLow-intensity pulsed ultrasoundMicrobubble3D printing scaffoldScaffold |
| spellingShingle | Lin Jin Jiali Shan Yanhong Hao Yingchun Wang Liping Liu Enhanced bone regeneration by low-intensity pulsed ultrasound and lipid microbubbles on PLGA/TCP 3D-printed scaffolds BMC Biotechnology Bone repair Low-intensity pulsed ultrasound Microbubble 3D printing scaffold Scaffold |
| title | Enhanced bone regeneration by low-intensity pulsed ultrasound and lipid microbubbles on PLGA/TCP 3D-printed scaffolds |
| title_full | Enhanced bone regeneration by low-intensity pulsed ultrasound and lipid microbubbles on PLGA/TCP 3D-printed scaffolds |
| title_fullStr | Enhanced bone regeneration by low-intensity pulsed ultrasound and lipid microbubbles on PLGA/TCP 3D-printed scaffolds |
| title_full_unstemmed | Enhanced bone regeneration by low-intensity pulsed ultrasound and lipid microbubbles on PLGA/TCP 3D-printed scaffolds |
| title_short | Enhanced bone regeneration by low-intensity pulsed ultrasound and lipid microbubbles on PLGA/TCP 3D-printed scaffolds |
| title_sort | enhanced bone regeneration by low intensity pulsed ultrasound and lipid microbubbles on plga tcp 3d printed scaffolds |
| topic | Bone repair Low-intensity pulsed ultrasound Microbubble 3D printing scaffold Scaffold |
| url | https://doi.org/10.1186/s12896-023-00783-9 |
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