Expanding the Benefits of Tnt1 for the Identification of Dominant Mutations in Polyploid Crops: A Single Allelic Mutation in the MsNAC39 Gene Produces Multifoliated Alfalfa
Most major crops are polyploid species and the production of genetically engineered cultivars normally requires the introgression of transgenic or gene-edited traits into elite germplasm. Thus, a main goal of plant research is the search of systems to identify dominant mutations. In this article, we...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2021-12-01
|
| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2021.805032/full |
| _version_ | 1818732605778952192 |
|---|---|
| author | Cintia Jozefkowicz Cintia Jozefkowicz Cristina Gómez Cristina Gómez Ariel Odorizzi Anelia Iantcheva Pascal Ratet Pascal Ratet Nicolás Ayub Nicolás Ayub Gabriela Soto Gabriela Soto |
| author_facet | Cintia Jozefkowicz Cintia Jozefkowicz Cristina Gómez Cristina Gómez Ariel Odorizzi Anelia Iantcheva Pascal Ratet Pascal Ratet Nicolás Ayub Nicolás Ayub Gabriela Soto Gabriela Soto |
| author_sort | Cintia Jozefkowicz |
| collection | DOAJ |
| description | Most major crops are polyploid species and the production of genetically engineered cultivars normally requires the introgression of transgenic or gene-edited traits into elite germplasm. Thus, a main goal of plant research is the search of systems to identify dominant mutations. In this article, we show that the Tnt1 element can be used to identify dominant mutations in allogamous tetraploid cultivated alfalfa. Specifically, we show that a single allelic mutation in the MsNAC39 gene produces multifoliate leaves (mfl) alfalfa plants, a pivot trait of breeding programs of this forage species. Finally, we discuss the potential application of a combination of preliminary screening of beneficial dominant mutants using Tnt1 mutant libraries and genome editing via the CRISPR/Cas9 system to identify target genes and to rapidly improve both autogamous and allogamous polyploid crops. |
| first_indexed | 2024-12-17T23:36:14Z |
| format | Article |
| id | doaj.art-c55205abb84340d281accf481c78979f |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-17T23:36:14Z |
| publishDate | 2021-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-c55205abb84340d281accf481c78979f2022-12-21T21:28:33ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2021-12-011210.3389/fpls.2021.805032805032Expanding the Benefits of Tnt1 for the Identification of Dominant Mutations in Polyploid Crops: A Single Allelic Mutation in the MsNAC39 Gene Produces Multifoliated AlfalfaCintia Jozefkowicz0Cintia Jozefkowicz1Cristina Gómez2Cristina Gómez3Ariel Odorizzi4Anelia Iantcheva5Pascal Ratet6Pascal Ratet7Nicolás Ayub8Nicolás Ayub9Gabriela Soto10Gabriela Soto11Instituto de Agrobiotecnología y Biología Molecular (INTA-CONICET), Buenos Aires, ArgentinaInstituto de Genética (IGEAF), Instituto Nacional de Tecnología Agropecuaria (INTA), Buenos Aires, ArgentinaInstituto de Agrobiotecnología y Biología Molecular (INTA-CONICET), Buenos Aires, ArgentinaInstituto de Genética (IGEAF), Instituto Nacional de Tecnología Agropecuaria (INTA), Buenos Aires, ArgentinaEstación Experimental Agropecuaria Manfredi, Instituto Nacional de Tecnología Agropecuaria (INTA), Córdoba, ArgentinaAgroBioInstitute, Agricultural Academy, Sofia, BulgariaUniversité Paris-Saclay, INRAE, CNRS, Université d’Évry, Institute of Plant Sciences Paris-Saclay (IPS2), Orsay, FranceUniversité de Paris, CNRS, INRAE, Institute of Plant Sciences Paris Saclay (IPS2), Orsay, FranceInstituto de Agrobiotecnología y Biología Molecular (INTA-CONICET), Buenos Aires, ArgentinaInstituto de Genética (IGEAF), Instituto Nacional de Tecnología Agropecuaria (INTA), Buenos Aires, ArgentinaInstituto de Agrobiotecnología y Biología Molecular (INTA-CONICET), Buenos Aires, ArgentinaInstituto de Genética (IGEAF), Instituto Nacional de Tecnología Agropecuaria (INTA), Buenos Aires, ArgentinaMost major crops are polyploid species and the production of genetically engineered cultivars normally requires the introgression of transgenic or gene-edited traits into elite germplasm. Thus, a main goal of plant research is the search of systems to identify dominant mutations. In this article, we show that the Tnt1 element can be used to identify dominant mutations in allogamous tetraploid cultivated alfalfa. Specifically, we show that a single allelic mutation in the MsNAC39 gene produces multifoliate leaves (mfl) alfalfa plants, a pivot trait of breeding programs of this forage species. Finally, we discuss the potential application of a combination of preliminary screening of beneficial dominant mutants using Tnt1 mutant libraries and genome editing via the CRISPR/Cas9 system to identify target genes and to rapidly improve both autogamous and allogamous polyploid crops.https://www.frontiersin.org/articles/10.3389/fpls.2021.805032/fulltransformationTnt1dominant mutationpolyploidyalfalfa |
| spellingShingle | Cintia Jozefkowicz Cintia Jozefkowicz Cristina Gómez Cristina Gómez Ariel Odorizzi Anelia Iantcheva Pascal Ratet Pascal Ratet Nicolás Ayub Nicolás Ayub Gabriela Soto Gabriela Soto Expanding the Benefits of Tnt1 for the Identification of Dominant Mutations in Polyploid Crops: A Single Allelic Mutation in the MsNAC39 Gene Produces Multifoliated Alfalfa Frontiers in Plant Science transformation Tnt1 dominant mutation polyploidy alfalfa |
| title | Expanding the Benefits of Tnt1 for the Identification of Dominant Mutations in Polyploid Crops: A Single Allelic Mutation in the MsNAC39 Gene Produces Multifoliated Alfalfa |
| title_full | Expanding the Benefits of Tnt1 for the Identification of Dominant Mutations in Polyploid Crops: A Single Allelic Mutation in the MsNAC39 Gene Produces Multifoliated Alfalfa |
| title_fullStr | Expanding the Benefits of Tnt1 for the Identification of Dominant Mutations in Polyploid Crops: A Single Allelic Mutation in the MsNAC39 Gene Produces Multifoliated Alfalfa |
| title_full_unstemmed | Expanding the Benefits of Tnt1 for the Identification of Dominant Mutations in Polyploid Crops: A Single Allelic Mutation in the MsNAC39 Gene Produces Multifoliated Alfalfa |
| title_short | Expanding the Benefits of Tnt1 for the Identification of Dominant Mutations in Polyploid Crops: A Single Allelic Mutation in the MsNAC39 Gene Produces Multifoliated Alfalfa |
| title_sort | expanding the benefits of tnt1 for the identification of dominant mutations in polyploid crops a single allelic mutation in the msnac39 gene produces multifoliated alfalfa |
| topic | transformation Tnt1 dominant mutation polyploidy alfalfa |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2021.805032/full |
| work_keys_str_mv | AT cintiajozefkowicz expandingthebenefitsoftnt1fortheidentificationofdominantmutationsinpolyploidcropsasingleallelicmutationinthemsnac39geneproducesmultifoliatedalfalfa AT cintiajozefkowicz expandingthebenefitsoftnt1fortheidentificationofdominantmutationsinpolyploidcropsasingleallelicmutationinthemsnac39geneproducesmultifoliatedalfalfa AT cristinagomez expandingthebenefitsoftnt1fortheidentificationofdominantmutationsinpolyploidcropsasingleallelicmutationinthemsnac39geneproducesmultifoliatedalfalfa AT cristinagomez expandingthebenefitsoftnt1fortheidentificationofdominantmutationsinpolyploidcropsasingleallelicmutationinthemsnac39geneproducesmultifoliatedalfalfa AT arielodorizzi expandingthebenefitsoftnt1fortheidentificationofdominantmutationsinpolyploidcropsasingleallelicmutationinthemsnac39geneproducesmultifoliatedalfalfa AT aneliaiantcheva expandingthebenefitsoftnt1fortheidentificationofdominantmutationsinpolyploidcropsasingleallelicmutationinthemsnac39geneproducesmultifoliatedalfalfa AT pascalratet expandingthebenefitsoftnt1fortheidentificationofdominantmutationsinpolyploidcropsasingleallelicmutationinthemsnac39geneproducesmultifoliatedalfalfa AT pascalratet expandingthebenefitsoftnt1fortheidentificationofdominantmutationsinpolyploidcropsasingleallelicmutationinthemsnac39geneproducesmultifoliatedalfalfa AT nicolasayub expandingthebenefitsoftnt1fortheidentificationofdominantmutationsinpolyploidcropsasingleallelicmutationinthemsnac39geneproducesmultifoliatedalfalfa AT nicolasayub expandingthebenefitsoftnt1fortheidentificationofdominantmutationsinpolyploidcropsasingleallelicmutationinthemsnac39geneproducesmultifoliatedalfalfa AT gabrielasoto expandingthebenefitsoftnt1fortheidentificationofdominantmutationsinpolyploidcropsasingleallelicmutationinthemsnac39geneproducesmultifoliatedalfalfa AT gabrielasoto expandingthebenefitsoftnt1fortheidentificationofdominantmutationsinpolyploidcropsasingleallelicmutationinthemsnac39geneproducesmultifoliatedalfalfa |