Relationship Between Expression of MicroRNA-150 and Tumor-associated Macrophages in Oral Squamous Cell Carcinoma
Objective To investigate the expression of miRNA-150 and IL-10 in TAMs and its clinicopathological significance in OSCC. Methods Different expression of miRNA in OSCC and control was screened by gene chip. The expression of miRNA-150 and CD68/IL-10 protein in oral tissues from 45 cases of OSCC and 3...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | zho |
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Magazine House of Cancer Research on Prevention and Treatment
2018-12-01
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| Series: | Zhongliu Fangzhi Yanjiu |
| Subjects: | |
| Online Access: | http://html.rhhz.net/ZLFZYJ/html/8578.2018.18.0931.htm |
| _version_ | 1818513315830169600 |
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| author | PENG Hongfeng WANG Yuanjie TIAN Songbo DONG Wei LIANG Yongqiang DONG Qing MA Dong |
| author_facet | PENG Hongfeng WANG Yuanjie TIAN Songbo DONG Wei LIANG Yongqiang DONG Qing MA Dong |
| author_sort | PENG Hongfeng |
| collection | DOAJ |
| description | Objective To investigate the expression of miRNA-150 and IL-10 in TAMs and its clinicopathological significance in OSCC. Methods Different expression of miRNA in OSCC and control was screened by gene chip. The expression of miRNA-150 and CD68/IL-10 protein in oral tissues from 45 cases of OSCC and 38 cases of normal oral mucosa were detected by qRT-PCR and dual immunofluorescence staining. The relationship between miRNA-150 expression and clinical data was analyzed. Results The expression of miRNA-150 and CD68/IL-10 in OSCC tissues was significantly higher than in normal tissues (P < 0.05). The infiltration of CD68+IL-10+ cells increased, and miRNA-150 was related to pathological staging, tumor differentiation and lymph node metastasis in patients with OSCC (P < 0.05). Conclusion The expression of miRNA-150 and TAM-derived IL-10 in OSCC is increased, suggesting that miRNA-150 may promote phenotype transformation of M2 macrophages participating in immunosuppressive function and oral tumorigenesis. |
| first_indexed | 2024-12-10T23:59:38Z |
| format | Article |
| id | doaj.art-c55d9fc283524952aeb56340b9f5aee0 |
| institution | Directory Open Access Journal |
| issn | 1000-8578 1000-8578 |
| language | zho |
| last_indexed | 2024-12-10T23:59:38Z |
| publishDate | 2018-12-01 |
| publisher | Magazine House of Cancer Research on Prevention and Treatment |
| record_format | Article |
| series | Zhongliu Fangzhi Yanjiu |
| spelling | doaj.art-c55d9fc283524952aeb56340b9f5aee02022-12-22T01:28:31ZzhoMagazine House of Cancer Research on Prevention and TreatmentZhongliu Fangzhi Yanjiu1000-85781000-85782018-12-01451299699910.3971/j.issn.1000-8578.2018.18.09318578.2018.18.0931Relationship Between Expression of MicroRNA-150 and Tumor-associated Macrophages in Oral Squamous Cell CarcinomaPENG Hongfeng0WANG Yuanjie1TIAN Songbo2DONG Wei3LIANG Yongqiang4DONG Qing5MA Dong6School of Stomatology, North China University of Science and Technology, Tangshan 063210, ChinaDepatement of Stomatology, Tangshan Vocational and Technical College, Tangshan 063000, ChinaDepartment of Oral Medicine, The Second Hospital of Hebei Medical University, Shijiazhuang 050000, ChinaSchool of Stomatology, North China University of Science and Technology, Tangshan 063210, ChinaSchool of Stomatology, North China University of Science and Technology, Tangshan 063210, ChinaSchool of Stomatology, North China University of Science and Technology, Tangshan 063210, ChinaSchool of Public Health, North China University of Science and Technology, Tangshan 063210, ChinaObjective To investigate the expression of miRNA-150 and IL-10 in TAMs and its clinicopathological significance in OSCC. Methods Different expression of miRNA in OSCC and control was screened by gene chip. The expression of miRNA-150 and CD68/IL-10 protein in oral tissues from 45 cases of OSCC and 38 cases of normal oral mucosa were detected by qRT-PCR and dual immunofluorescence staining. The relationship between miRNA-150 expression and clinical data was analyzed. Results The expression of miRNA-150 and CD68/IL-10 in OSCC tissues was significantly higher than in normal tissues (P < 0.05). The infiltration of CD68+IL-10+ cells increased, and miRNA-150 was related to pathological staging, tumor differentiation and lymph node metastasis in patients with OSCC (P < 0.05). Conclusion The expression of miRNA-150 and TAM-derived IL-10 in OSCC is increased, suggesting that miRNA-150 may promote phenotype transformation of M2 macrophages participating in immunosuppressive function and oral tumorigenesis.http://html.rhhz.net/ZLFZYJ/html/8578.2018.18.0931.htmoral squamous cell carcinomail-10tumor-associated macrophages |
| spellingShingle | PENG Hongfeng WANG Yuanjie TIAN Songbo DONG Wei LIANG Yongqiang DONG Qing MA Dong Relationship Between Expression of MicroRNA-150 and Tumor-associated Macrophages in Oral Squamous Cell Carcinoma Zhongliu Fangzhi Yanjiu oral squamous cell carcinoma il-10 tumor-associated macrophages |
| title | Relationship Between Expression of MicroRNA-150 and Tumor-associated Macrophages in Oral Squamous Cell Carcinoma |
| title_full | Relationship Between Expression of MicroRNA-150 and Tumor-associated Macrophages in Oral Squamous Cell Carcinoma |
| title_fullStr | Relationship Between Expression of MicroRNA-150 and Tumor-associated Macrophages in Oral Squamous Cell Carcinoma |
| title_full_unstemmed | Relationship Between Expression of MicroRNA-150 and Tumor-associated Macrophages in Oral Squamous Cell Carcinoma |
| title_short | Relationship Between Expression of MicroRNA-150 and Tumor-associated Macrophages in Oral Squamous Cell Carcinoma |
| title_sort | relationship between expression of microrna 150 and tumor associated macrophages in oral squamous cell carcinoma |
| topic | oral squamous cell carcinoma il-10 tumor-associated macrophages |
| url | http://html.rhhz.net/ZLFZYJ/html/8578.2018.18.0931.htm |
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