Expression of Cell-Cycle Regulatory Proteins pRb, Cyclin D1, and p53 Is Not Associated with Recurrence Rates of Equine Sarcoids
Sarcoids are among the most common tumors diagnosed in equids; their association with bovine papillomaviruses (BPV) infection has been widely reported, but the mechanism of carcinogenesis has not been fully elucidated. To verify whether BPV infection causes dysregulation of the pRb-Cyclin D1-p16<...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-09-01
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| Series: | Veterinary Sciences |
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| Online Access: | https://www.mdpi.com/2306-7381/9/9/474 |
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| author | Giorgia Tura Barbara Brunetti Elena Brigandì Riccardo Rinnovati Giuseppe Sarli Giancarlo Avallone Luisa Vera Muscatello Roberto Marcello La Ragione Andy E. Durham Barbara Bacci |
| author_facet | Giorgia Tura Barbara Brunetti Elena Brigandì Riccardo Rinnovati Giuseppe Sarli Giancarlo Avallone Luisa Vera Muscatello Roberto Marcello La Ragione Andy E. Durham Barbara Bacci |
| author_sort | Giorgia Tura |
| collection | DOAJ |
| description | Sarcoids are among the most common tumors diagnosed in equids; their association with bovine papillomaviruses (BPV) infection has been widely reported, but the mechanism of carcinogenesis has not been fully elucidated. To verify whether BPV infection causes dysregulation of the pRb-Cyclin D1-p16<sup>CDKN2A</sup>-p53 pathway as reported for human papillomavirus (HPV), the study employed immunohistochemistry to test 55 equine sarcoid biopsies for the expression of pRb, Cyclin D1, and p53 cell cycle regulatory proteins and to evaluate the proliferative rate through Ki67. High Cyclin D1 and pRb expression were observed in 51% and 80% of cases, respectively, while low expression was observed in 49% and 20% of cases, respectively. Significantly higher Ki67 proliferation indexes were observed in fibroblastic, nodular, and mixed sarcoids compared to the occult and verrucous. High proliferation was significantly associated with high Cyclin D1 expression. In contrast with previous studies, p53 positivity was not observed in the cases examined in this study. Moreover, follow-up analysis revealed that fibroblastic, mixed sarcoids were associated with significantly higher local recurrence rates while the verrucous subtype was associated with higher rates of new sarcoid development at distant sites. |
| first_indexed | 2024-03-09T22:15:39Z |
| format | Article |
| id | doaj.art-c563433f5c62449989b1a9f783ba8614 |
| institution | Directory Open Access Journal |
| issn | 2306-7381 |
| language | English |
| last_indexed | 2024-03-09T22:15:39Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Veterinary Sciences |
| spelling | doaj.art-c563433f5c62449989b1a9f783ba86142023-11-23T19:24:00ZengMDPI AGVeterinary Sciences2306-73812022-09-019947410.3390/vetsci9090474Expression of Cell-Cycle Regulatory Proteins pRb, Cyclin D1, and p53 Is Not Associated with Recurrence Rates of Equine SarcoidsGiorgia Tura0Barbara Brunetti1Elena Brigandì2Riccardo Rinnovati3Giuseppe Sarli4Giancarlo Avallone5Luisa Vera Muscatello6Roberto Marcello La Ragione7Andy E. Durham8Barbara Bacci9Department of Veterinary Medical Sciences, University of Bologna, 40064 Bologna, ItalyDepartment of Veterinary Medical Sciences, University of Bologna, 40064 Bologna, ItalyDepartment of Veterinary Medical Sciences, University of Bologna, 40064 Bologna, ItalyDepartment of Veterinary Medical Sciences, University of Bologna, 40064 Bologna, ItalyDepartment of Veterinary Medical Sciences, University of Bologna, 40064 Bologna, ItalyDepartment of Veterinary Medical Sciences, University of Bologna, 40064 Bologna, ItalyDepartment of Veterinary Medical Sciences, University of Bologna, 40064 Bologna, ItalySchool of Veterinary Medicine and Science, University of Surrey, Guildford GU2 7XH, UKSchool of Veterinary Medicine and Science, University of Surrey, Guildford GU2 7XH, UKDepartment of Veterinary Medical Sciences, University of Bologna, 40064 Bologna, ItalySarcoids are among the most common tumors diagnosed in equids; their association with bovine papillomaviruses (BPV) infection has been widely reported, but the mechanism of carcinogenesis has not been fully elucidated. To verify whether BPV infection causes dysregulation of the pRb-Cyclin D1-p16<sup>CDKN2A</sup>-p53 pathway as reported for human papillomavirus (HPV), the study employed immunohistochemistry to test 55 equine sarcoid biopsies for the expression of pRb, Cyclin D1, and p53 cell cycle regulatory proteins and to evaluate the proliferative rate through Ki67. High Cyclin D1 and pRb expression were observed in 51% and 80% of cases, respectively, while low expression was observed in 49% and 20% of cases, respectively. Significantly higher Ki67 proliferation indexes were observed in fibroblastic, nodular, and mixed sarcoids compared to the occult and verrucous. High proliferation was significantly associated with high Cyclin D1 expression. In contrast with previous studies, p53 positivity was not observed in the cases examined in this study. Moreover, follow-up analysis revealed that fibroblastic, mixed sarcoids were associated with significantly higher local recurrence rates while the verrucous subtype was associated with higher rates of new sarcoid development at distant sites.https://www.mdpi.com/2306-7381/9/9/474equidsarcoidbovine papillomavirusp16<sup>CDKN2A</sup>pRbCyclin D1 |
| spellingShingle | Giorgia Tura Barbara Brunetti Elena Brigandì Riccardo Rinnovati Giuseppe Sarli Giancarlo Avallone Luisa Vera Muscatello Roberto Marcello La Ragione Andy E. Durham Barbara Bacci Expression of Cell-Cycle Regulatory Proteins pRb, Cyclin D1, and p53 Is Not Associated with Recurrence Rates of Equine Sarcoids Veterinary Sciences equid sarcoid bovine papillomavirus p16<sup>CDKN2A</sup> pRb Cyclin D1 |
| title | Expression of Cell-Cycle Regulatory Proteins pRb, Cyclin D1, and p53 Is Not Associated with Recurrence Rates of Equine Sarcoids |
| title_full | Expression of Cell-Cycle Regulatory Proteins pRb, Cyclin D1, and p53 Is Not Associated with Recurrence Rates of Equine Sarcoids |
| title_fullStr | Expression of Cell-Cycle Regulatory Proteins pRb, Cyclin D1, and p53 Is Not Associated with Recurrence Rates of Equine Sarcoids |
| title_full_unstemmed | Expression of Cell-Cycle Regulatory Proteins pRb, Cyclin D1, and p53 Is Not Associated with Recurrence Rates of Equine Sarcoids |
| title_short | Expression of Cell-Cycle Regulatory Proteins pRb, Cyclin D1, and p53 Is Not Associated with Recurrence Rates of Equine Sarcoids |
| title_sort | expression of cell cycle regulatory proteins prb cyclin d1 and p53 is not associated with recurrence rates of equine sarcoids |
| topic | equid sarcoid bovine papillomavirus p16<sup>CDKN2A</sup> pRb Cyclin D1 |
| url | https://www.mdpi.com/2306-7381/9/9/474 |
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