An Efficient Nitroblue Tetrazolium Staining and Bright-Field Microscopy Based Method for Detecting and Quantifying Intracellular Reactive Oxygen Species in Oocytes, Cumulus Cells and Embryos

An Efficient Nitroblue Tetrazolium Staining and Bright-Field Microscopy Based Method for Detecting and Quantifying Intracellular Reactive Oxygen Species in Oocytes, Cumulus Cells and Embryos

Assessment of intracellular reactive oxygen species (ROS) is important for evaluating the developmental ability of cumulus-oocyte complexes (COC) and embryos. Although, fluorescence-based 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA) staining method is used widely for detecting intracellular...

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Bibliographic Details
Main Authors: Pradeep K. Javvaji, Arindam Dhali, Joseph R. Francis, Atul P. Kolte, Anjumoni Mech, Sudhir C. Roy, Ashish Mishra, Raghavendra Bhatta
Format: Article
Language:English
Published: Frontiers Media S.A. 2020-08-01
Series:Frontiers in Cell and Developmental Biology
Subjects:
intracellular ROS
detection
NBT
oocyte
cumulus cell
embryo
Online Access:https://www.frontiersin.org/article/10.3389/fcell.2020.00764/full

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