Kinetics of Pectin Biopolymer Facial Erosion Characterized by Fluorescent Tracer Microfluidics
Pectin is a plant-derived heteropolysaccharide that has been implicated in drug development, tissue engineering, and visceral organ repair. Pectin demonstrates remarkable biostability in a variety of physiologic environments but is biodegradable in water. To understand the dynamics of pectin biodegr...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-09-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/14/18/3911 |
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| author | Matthew W. Liao Betty S. Liu Joseph Sutlive Willi L. Wagner Hassan A. Khalil Zi Chen Maximilian Ackermann Steven J. Mentzer |
| author_facet | Matthew W. Liao Betty S. Liu Joseph Sutlive Willi L. Wagner Hassan A. Khalil Zi Chen Maximilian Ackermann Steven J. Mentzer |
| author_sort | Matthew W. Liao |
| collection | DOAJ |
| description | Pectin is a plant-derived heteropolysaccharide that has been implicated in drug development, tissue engineering, and visceral organ repair. Pectin demonstrates remarkable biostability in a variety of physiologic environments but is biodegradable in water. To understand the dynamics of pectin biodegradation in basic environments, we developed a microfluidics system that facilitated the quantitative comparison of pectin films exposed to facial erosion. Pectin biodegradation was assessed using fluorescein tracer embedded in pectin, trypan blue quenching of released fluorescence, and highly sensitive microfluorimetry. The microfluidic perfusate, delivered through 6 um-pore synthetic membrane interface, demonstrated nonlinear erosion of the pectin film; 75% of tracer was released in 28 h. The microfluidics system was used to identify potential modifiers of pectin erosion. The polyphenolic compound tannic acid, loaded into citrus pectin films, demonstrated a dose-dependent decrease in pectin erosion. Tannic acid had no detectable impact on the physical properties of citrus pectin including adhesivity and cohesion. In contrast, tannic acid weakened the burst strength and cohesion of pectins derived from soy bean and potato sources. We conclude that facial erosion may explain the biostability of citrus pectin on visceral organ surfaces as well as provide a useful method for identifying modifiers of citrus pectin biodegradation. |
| first_indexed | 2024-03-09T22:44:52Z |
| format | Article |
| id | doaj.art-c569ba82540e43f1b7e56b28075d20ce |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-09T22:44:52Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-c569ba82540e43f1b7e56b28075d20ce2023-11-23T18:32:00ZengMDPI AGPolymers2073-43602022-09-011418391110.3390/polym14183911Kinetics of Pectin Biopolymer Facial Erosion Characterized by Fluorescent Tracer MicrofluidicsMatthew W. Liao0Betty S. Liu1Joseph Sutlive2Willi L. Wagner3Hassan A. Khalil4Zi Chen5Maximilian Ackermann6Steven J. Mentzer7Laboratory of Adaptive and Regenerative Biology, Brigham & Women’s Hospital, Harvard Medical School, Boston, MA 02115, USALaboratory of Adaptive and Regenerative Biology, Brigham & Women’s Hospital, Harvard Medical School, Boston, MA 02115, USALaboratory of Adaptive and Regenerative Biology, Brigham & Women’s Hospital, Harvard Medical School, Boston, MA 02115, USALaboratory of Adaptive and Regenerative Biology, Brigham & Women’s Hospital, Harvard Medical School, Boston, MA 02115, USALaboratory of Adaptive and Regenerative Biology, Brigham & Women’s Hospital, Harvard Medical School, Boston, MA 02115, USALaboratory of Adaptive and Regenerative Biology, Brigham & Women’s Hospital, Harvard Medical School, Boston, MA 02115, USAInstitute of Functional and Clinical Anatomy, University Medical Center of the Johannes Gutenberg-University Mainz, 55131 Mainz, GermanyLaboratory of Adaptive and Regenerative Biology, Brigham & Women’s Hospital, Harvard Medical School, Boston, MA 02115, USAPectin is a plant-derived heteropolysaccharide that has been implicated in drug development, tissue engineering, and visceral organ repair. Pectin demonstrates remarkable biostability in a variety of physiologic environments but is biodegradable in water. To understand the dynamics of pectin biodegradation in basic environments, we developed a microfluidics system that facilitated the quantitative comparison of pectin films exposed to facial erosion. Pectin biodegradation was assessed using fluorescein tracer embedded in pectin, trypan blue quenching of released fluorescence, and highly sensitive microfluorimetry. The microfluidic perfusate, delivered through 6 um-pore synthetic membrane interface, demonstrated nonlinear erosion of the pectin film; 75% of tracer was released in 28 h. The microfluidics system was used to identify potential modifiers of pectin erosion. The polyphenolic compound tannic acid, loaded into citrus pectin films, demonstrated a dose-dependent decrease in pectin erosion. Tannic acid had no detectable impact on the physical properties of citrus pectin including adhesivity and cohesion. In contrast, tannic acid weakened the burst strength and cohesion of pectins derived from soy bean and potato sources. We conclude that facial erosion may explain the biostability of citrus pectin on visceral organ surfaces as well as provide a useful method for identifying modifiers of citrus pectin biodegradation.https://www.mdpi.com/2073-4360/14/18/3911pectintannic acidkineticsbiostabilitybiodegradationerosion |
| spellingShingle | Matthew W. Liao Betty S. Liu Joseph Sutlive Willi L. Wagner Hassan A. Khalil Zi Chen Maximilian Ackermann Steven J. Mentzer Kinetics of Pectin Biopolymer Facial Erosion Characterized by Fluorescent Tracer Microfluidics Polymers pectin tannic acid kinetics biostability biodegradation erosion |
| title | Kinetics of Pectin Biopolymer Facial Erosion Characterized by Fluorescent Tracer Microfluidics |
| title_full | Kinetics of Pectin Biopolymer Facial Erosion Characterized by Fluorescent Tracer Microfluidics |
| title_fullStr | Kinetics of Pectin Biopolymer Facial Erosion Characterized by Fluorescent Tracer Microfluidics |
| title_full_unstemmed | Kinetics of Pectin Biopolymer Facial Erosion Characterized by Fluorescent Tracer Microfluidics |
| title_short | Kinetics of Pectin Biopolymer Facial Erosion Characterized by Fluorescent Tracer Microfluidics |
| title_sort | kinetics of pectin biopolymer facial erosion characterized by fluorescent tracer microfluidics |
| topic | pectin tannic acid kinetics biostability biodegradation erosion |
| url | https://www.mdpi.com/2073-4360/14/18/3911 |
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