Comparison of single radial immunodiffusion and ELISA for the quantification of immunoglobulin G in bovine colostrum, milk and calf sera
The overall objective was to compare immunoglobulin G (IgG) concentrations measured by single radial immunodiffusion (sRID) and ELISA-based methods in samples of bovine colostrum and transition milk from contrasting breed types (Limousin × Friesian (n = 10) and Holstein (n = 10)). Jugular blood samp...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2018-01-01
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| Series: | Journal of Applied Animal Research |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/09712119.2017.1394860 |
| _version_ | 1818973037967441920 |
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| author | Amanda Dunn Catherine Duffy Alan Gordon Steven Morrison Anastasio Argűello Michael Welsh Bernadette Earley |
| author_facet | Amanda Dunn Catherine Duffy Alan Gordon Steven Morrison Anastasio Argűello Michael Welsh Bernadette Earley |
| author_sort | Amanda Dunn |
| collection | DOAJ |
| description | The overall objective was to compare immunoglobulin G (IgG) concentrations measured by single radial immunodiffusion (sRID) and ELISA-based methods in samples of bovine colostrum and transition milk from contrasting breed types (Limousin × Friesian (n = 10) and Holstein (n = 10)). Jugular blood samples were collected at 48 h post-birth from beef (n = 10) and dairy (n = 10) calves and sera harvested subsequent to colostrum consumption. Absolute colostrum IgG values determined by ELISA showed poor agreement with mean (SD) IgG values measured using sRID, fixed bias (sRID – ELISA) was 31.89 (±9.84) mg/mL; having wide limits of agreement (12.61–51.17) and a low concordance coefficient (0.26). The agreement between ELISA and sRID when measuring serum IgG was greater than that of colostrum, fixed bias (sRID – ELISA) was 12.36 (±6.60) mg/mL; having narrower limits of agreement (−0.58 to 25.30) and serum IgG concentrations had a greater concordance coefficient (0.44) between samples. Calf sera IgG measured using the indirect zinc sulphate turbidity test showed a strong correlation with the sRID and ELISA methods (P < .001), R2 = 0.78 and R2 = 0.77 respectively. Overall, the ELISA and sRID methodologies had a strong positive association with almost a twofold (1.8) difference between values; therefore, they provide diverse absolute values of IgG concentration. |
| first_indexed | 2024-12-20T15:17:48Z |
| format | Article |
| id | doaj.art-c56a9f3d35b2434eb32743455a6b2f18 |
| institution | Directory Open Access Journal |
| issn | 0971-2119 0974-1844 |
| language | English |
| last_indexed | 2024-12-20T15:17:48Z |
| publishDate | 2018-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Applied Animal Research |
| spelling | doaj.art-c56a9f3d35b2434eb32743455a6b2f182022-12-21T19:36:08ZengTaylor & Francis GroupJournal of Applied Animal Research0971-21190974-18442018-01-0146175876510.1080/09712119.2017.13948601394860Comparison of single radial immunodiffusion and ELISA for the quantification of immunoglobulin G in bovine colostrum, milk and calf seraAmanda Dunn0Catherine Duffy1Alan Gordon2Steven Morrison3Anastasio Argűello4Michael Welsh5Bernadette Earley6Agri-food and Bio-sciences InstituteVeterinary Science DivisionAgri-food and Bio-sciences InstituteAgri-food and Bio-sciences InstituteAnimal and Grassland Research and Innovation Centre (AGRIC), Teagasc, GrangeSisafAnimal and Grassland Research and Innovation Centre (AGRIC), Teagasc, GrangeThe overall objective was to compare immunoglobulin G (IgG) concentrations measured by single radial immunodiffusion (sRID) and ELISA-based methods in samples of bovine colostrum and transition milk from contrasting breed types (Limousin × Friesian (n = 10) and Holstein (n = 10)). Jugular blood samples were collected at 48 h post-birth from beef (n = 10) and dairy (n = 10) calves and sera harvested subsequent to colostrum consumption. Absolute colostrum IgG values determined by ELISA showed poor agreement with mean (SD) IgG values measured using sRID, fixed bias (sRID – ELISA) was 31.89 (±9.84) mg/mL; having wide limits of agreement (12.61–51.17) and a low concordance coefficient (0.26). The agreement between ELISA and sRID when measuring serum IgG was greater than that of colostrum, fixed bias (sRID – ELISA) was 12.36 (±6.60) mg/mL; having narrower limits of agreement (−0.58 to 25.30) and serum IgG concentrations had a greater concordance coefficient (0.44) between samples. Calf sera IgG measured using the indirect zinc sulphate turbidity test showed a strong correlation with the sRID and ELISA methods (P < .001), R2 = 0.78 and R2 = 0.77 respectively. Overall, the ELISA and sRID methodologies had a strong positive association with almost a twofold (1.8) difference between values; therefore, they provide diverse absolute values of IgG concentration.http://dx.doi.org/10.1080/09712119.2017.1394860ELISAsingle radial immunodiffusionzinc sulphate turbidity testBrix refractometerIgGcalfcolostrum |
| spellingShingle | Amanda Dunn Catherine Duffy Alan Gordon Steven Morrison Anastasio Argűello Michael Welsh Bernadette Earley Comparison of single radial immunodiffusion and ELISA for the quantification of immunoglobulin G in bovine colostrum, milk and calf sera Journal of Applied Animal Research ELISA single radial immunodiffusion zinc sulphate turbidity test Brix refractometer IgG calf colostrum |
| title | Comparison of single radial immunodiffusion and ELISA for the quantification of immunoglobulin G in bovine colostrum, milk and calf sera |
| title_full | Comparison of single radial immunodiffusion and ELISA for the quantification of immunoglobulin G in bovine colostrum, milk and calf sera |
| title_fullStr | Comparison of single radial immunodiffusion and ELISA for the quantification of immunoglobulin G in bovine colostrum, milk and calf sera |
| title_full_unstemmed | Comparison of single radial immunodiffusion and ELISA for the quantification of immunoglobulin G in bovine colostrum, milk and calf sera |
| title_short | Comparison of single radial immunodiffusion and ELISA for the quantification of immunoglobulin G in bovine colostrum, milk and calf sera |
| title_sort | comparison of single radial immunodiffusion and elisa for the quantification of immunoglobulin g in bovine colostrum milk and calf sera |
| topic | ELISA single radial immunodiffusion zinc sulphate turbidity test Brix refractometer IgG calf colostrum |
| url | http://dx.doi.org/10.1080/09712119.2017.1394860 |
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