Multi-Sample Preparation Assay for Isolation of Nucleic Acids Using Bio-Silica with Syringe Filters
The spin-column system for the isolation of nucleic acids (NAs) from multiple samples presents the inconvenience of repeated experimentation, time-consumption, and the risk of contamination in the process of the spin-column exchange. Herein, we propose a convenient and universal assay that can be us...
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MDPI AG
2020-08-01
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| Series: | Micromachines |
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| Online Access: | https://www.mdpi.com/2072-666X/11/9/823 |
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| author | Geun Su Noh Huifang Liu Myoung Gyu Kim Zhen Qiao Yoon Ok Jang Yong Shin |
| author_facet | Geun Su Noh Huifang Liu Myoung Gyu Kim Zhen Qiao Yoon Ok Jang Yong Shin |
| author_sort | Geun Su Noh |
| collection | DOAJ |
| description | The spin-column system for the isolation of nucleic acids (NAs) from multiple samples presents the inconvenience of repeated experimentation, time-consumption, and the risk of contamination in the process of the spin-column exchange. Herein, we propose a convenient and universal assay that can be used to diagnose multiple pathogens using a multi-sample preparation assay. The multi-sample preparation assay combines a 96-well filter/membrane plate, a bio-micromaterial lattice-like micro amine-functional diatomaceous earth (D-APDMS), and homobifunctional imidoesters (HI) for the processing of pathogen enrichment and extraction for multiple samples simultaneously. The purity and quantity of the extracted NAs from pathogens (<i>E. coli</i> and <i>Brucella</i>) using the proposed assay is superior to that of the commercialized spin-column kit. The assay also does not require the replacement of several collection tubes during the reaction processing. For the multi-sample testing, we used as many as six samples simultaneously with the proposed assay. This assay can simultaneously separate up to 96 NAs from one plate, and the use of multichannel pipettes allows faster and simpler experimentation. Therefore, we believe it is a convenient and easy process, and can be easily integrated with other detection methods for clinical diagnostics. |
| first_indexed | 2024-03-10T16:43:15Z |
| format | Article |
| id | doaj.art-c56bae6cb00c4c97bdf54d25920c7961 |
| institution | Directory Open Access Journal |
| issn | 2072-666X |
| language | English |
| last_indexed | 2024-03-10T16:43:15Z |
| publishDate | 2020-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Micromachines |
| spelling | doaj.art-c56bae6cb00c4c97bdf54d25920c79612023-11-20T11:53:54ZengMDPI AGMicromachines2072-666X2020-08-0111982310.3390/mi11090823Multi-Sample Preparation Assay for Isolation of Nucleic Acids Using Bio-Silica with Syringe FiltersGeun Su Noh0Huifang Liu1Myoung Gyu Kim2Zhen Qiao3Yoon Ok Jang4Yong Shin5Department of Convergence Medicine, Asan Medical Institute of Convergence Science and Technology (AMIST), University of Ulsan College of Medicine, 88 Olympicro-43gil, Songpa-gu, Seoul 05505, KoreaDepartment of Convergence Medicine, Asan Medical Institute of Convergence Science and Technology (AMIST), University of Ulsan College of Medicine, 88 Olympicro-43gil, Songpa-gu, Seoul 05505, KoreaDepartment of Convergence Medicine, Asan Medical Institute of Convergence Science and Technology (AMIST), University of Ulsan College of Medicine, 88 Olympicro-43gil, Songpa-gu, Seoul 05505, KoreaDepartment of Convergence Medicine, Asan Medical Institute of Convergence Science and Technology (AMIST), University of Ulsan College of Medicine, 88 Olympicro-43gil, Songpa-gu, Seoul 05505, KoreaDepartment of Convergence Medicine, Asan Medical Institute of Convergence Science and Technology (AMIST), University of Ulsan College of Medicine, 88 Olympicro-43gil, Songpa-gu, Seoul 05505, KoreaDepartment of Convergence Medicine, Asan Medical Institute of Convergence Science and Technology (AMIST), University of Ulsan College of Medicine, 88 Olympicro-43gil, Songpa-gu, Seoul 05505, KoreaThe spin-column system for the isolation of nucleic acids (NAs) from multiple samples presents the inconvenience of repeated experimentation, time-consumption, and the risk of contamination in the process of the spin-column exchange. Herein, we propose a convenient and universal assay that can be used to diagnose multiple pathogens using a multi-sample preparation assay. The multi-sample preparation assay combines a 96-well filter/membrane plate, a bio-micromaterial lattice-like micro amine-functional diatomaceous earth (D-APDMS), and homobifunctional imidoesters (HI) for the processing of pathogen enrichment and extraction for multiple samples simultaneously. The purity and quantity of the extracted NAs from pathogens (<i>E. coli</i> and <i>Brucella</i>) using the proposed assay is superior to that of the commercialized spin-column kit. The assay also does not require the replacement of several collection tubes during the reaction processing. For the multi-sample testing, we used as many as six samples simultaneously with the proposed assay. This assay can simultaneously separate up to 96 NAs from one plate, and the use of multichannel pipettes allows faster and simpler experimentation. Therefore, we believe it is a convenient and easy process, and can be easily integrated with other detection methods for clinical diagnostics.https://www.mdpi.com/2072-666X/11/9/823multi-sample preparation assaypathogen extractionnucleic acid isolation |
| spellingShingle | Geun Su Noh Huifang Liu Myoung Gyu Kim Zhen Qiao Yoon Ok Jang Yong Shin Multi-Sample Preparation Assay for Isolation of Nucleic Acids Using Bio-Silica with Syringe Filters Micromachines multi-sample preparation assay pathogen extraction nucleic acid isolation |
| title | Multi-Sample Preparation Assay for Isolation of Nucleic Acids Using Bio-Silica with Syringe Filters |
| title_full | Multi-Sample Preparation Assay for Isolation of Nucleic Acids Using Bio-Silica with Syringe Filters |
| title_fullStr | Multi-Sample Preparation Assay for Isolation of Nucleic Acids Using Bio-Silica with Syringe Filters |
| title_full_unstemmed | Multi-Sample Preparation Assay for Isolation of Nucleic Acids Using Bio-Silica with Syringe Filters |
| title_short | Multi-Sample Preparation Assay for Isolation of Nucleic Acids Using Bio-Silica with Syringe Filters |
| title_sort | multi sample preparation assay for isolation of nucleic acids using bio silica with syringe filters |
| topic | multi-sample preparation assay pathogen extraction nucleic acid isolation |
| url | https://www.mdpi.com/2072-666X/11/9/823 |
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