Functionalized Silica Star-Shaped Nanoparticles and Human Mesenchymal Stem Cells: An In Vitro Model
The biomedical translational applications of functionalized nanoparticles require comprehensive studies on their effect on human stem cells. Here, we have tested neat star-shaped mesoporous silica nanoparticles (s-MSN) and their chemically functionalized derivates; we examined nanoparticles (NPs) wi...
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MDPI AG
2021-03-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/11/3/779 |
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| author | Chiara Argentati Francesco Morena Chiara Fontana Ilaria Tortorella Carla Emiliani Loredana Latterini Giulia Zampini Sabata Martino |
| author_facet | Chiara Argentati Francesco Morena Chiara Fontana Ilaria Tortorella Carla Emiliani Loredana Latterini Giulia Zampini Sabata Martino |
| author_sort | Chiara Argentati |
| collection | DOAJ |
| description | The biomedical translational applications of functionalized nanoparticles require comprehensive studies on their effect on human stem cells. Here, we have tested neat star-shaped mesoporous silica nanoparticles (s-MSN) and their chemically functionalized derivates; we examined nanoparticles (NPs) with similar dimensions but different surface chemistry, due to the amino groups grafted on silica nanoparticles (s-MSN-NH<sub>2</sub>), and gold nanoseeds chemically adsorbed on silica nanoparticles (s-MSN-Au). The different samples were dropped on glass coverslips to obtain a homogeneous deposition differing only for NPs’ chemical functionalization and suitable for long-term culture of human Bone Marrow–Mesenchymal stem cells (hBM-MSCs) and Adipose stem cells (hASCs). Our model allowed us to demonstrate that hBM-MSCs and hASCs have comparable growth curves, viability, and canonical Vinculin Focal adhesion spots on functionalized s-MSN-NH<sub>2</sub> and s-MSN-Au as on neat s-MSN and control systems, but also to show morphological changes on all NP types compared to the control counterparts. The new shape was stem-cell-specific and was maintained on all types of NPs. Compared to the other NPs, s-MSN-Au exerted a small genotoxic effect on both stem cell types, which, however, did not affect the stem cell behavior, likely due to a peculiar stem cell metabolic restoration response. |
| first_indexed | 2024-03-10T13:07:08Z |
| format | Article |
| id | doaj.art-c56d832f7c4447a5a32d43c9220bdd50 |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-10T13:07:08Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-c56d832f7c4447a5a32d43c9220bdd502023-11-21T11:05:28ZengMDPI AGNanomaterials2079-49912021-03-0111377910.3390/nano11030779Functionalized Silica Star-Shaped Nanoparticles and Human Mesenchymal Stem Cells: An In Vitro ModelChiara Argentati0Francesco Morena1Chiara Fontana2Ilaria Tortorella3Carla Emiliani4Loredana Latterini5Giulia Zampini6Sabata Martino7Department of Chemistry, Biology and Biotechnology, University of Perugia, Via del Giochetto, 06123 Perugia, ItalyDepartment of Chemistry, Biology and Biotechnology, University of Perugia, Via del Giochetto, 06123 Perugia, ItalyDepartment of Chemistry, Biology and Biotechnology, University of Perugia, Via Elce di Sotto 8, 06123 Perugia, ItalyDepartment of Chemistry, Biology and Biotechnology, University of Perugia, Via del Giochetto, 06123 Perugia, ItalyDepartment of Chemistry, Biology and Biotechnology, University of Perugia, Via del Giochetto, 06123 Perugia, ItalyDepartment of Chemistry, Biology and Biotechnology, University of Perugia, Via Elce di Sotto 8, 06123 Perugia, ItalyDepartment of Chemistry, Biology and Biotechnology, University of Perugia, Via Elce di Sotto 8, 06123 Perugia, ItalyDepartment of Chemistry, Biology and Biotechnology, University of Perugia, Via del Giochetto, 06123 Perugia, ItalyThe biomedical translational applications of functionalized nanoparticles require comprehensive studies on their effect on human stem cells. Here, we have tested neat star-shaped mesoporous silica nanoparticles (s-MSN) and their chemically functionalized derivates; we examined nanoparticles (NPs) with similar dimensions but different surface chemistry, due to the amino groups grafted on silica nanoparticles (s-MSN-NH<sub>2</sub>), and gold nanoseeds chemically adsorbed on silica nanoparticles (s-MSN-Au). The different samples were dropped on glass coverslips to obtain a homogeneous deposition differing only for NPs’ chemical functionalization and suitable for long-term culture of human Bone Marrow–Mesenchymal stem cells (hBM-MSCs) and Adipose stem cells (hASCs). Our model allowed us to demonstrate that hBM-MSCs and hASCs have comparable growth curves, viability, and canonical Vinculin Focal adhesion spots on functionalized s-MSN-NH<sub>2</sub> and s-MSN-Au as on neat s-MSN and control systems, but also to show morphological changes on all NP types compared to the control counterparts. The new shape was stem-cell-specific and was maintained on all types of NPs. Compared to the other NPs, s-MSN-Au exerted a small genotoxic effect on both stem cell types, which, however, did not affect the stem cell behavior, likely due to a peculiar stem cell metabolic restoration response.https://www.mdpi.com/2079-4991/11/3/779mesenchymal stem cellsstem-cell–nanomaterials interactionsilica star-shaped nanoparticlesgold nanoparticles |
| spellingShingle | Chiara Argentati Francesco Morena Chiara Fontana Ilaria Tortorella Carla Emiliani Loredana Latterini Giulia Zampini Sabata Martino Functionalized Silica Star-Shaped Nanoparticles and Human Mesenchymal Stem Cells: An In Vitro Model Nanomaterials mesenchymal stem cells stem-cell–nanomaterials interaction silica star-shaped nanoparticles gold nanoparticles |
| title | Functionalized Silica Star-Shaped Nanoparticles and Human Mesenchymal Stem Cells: An In Vitro Model |
| title_full | Functionalized Silica Star-Shaped Nanoparticles and Human Mesenchymal Stem Cells: An In Vitro Model |
| title_fullStr | Functionalized Silica Star-Shaped Nanoparticles and Human Mesenchymal Stem Cells: An In Vitro Model |
| title_full_unstemmed | Functionalized Silica Star-Shaped Nanoparticles and Human Mesenchymal Stem Cells: An In Vitro Model |
| title_short | Functionalized Silica Star-Shaped Nanoparticles and Human Mesenchymal Stem Cells: An In Vitro Model |
| title_sort | functionalized silica star shaped nanoparticles and human mesenchymal stem cells an in vitro model |
| topic | mesenchymal stem cells stem-cell–nanomaterials interaction silica star-shaped nanoparticles gold nanoparticles |
| url | https://www.mdpi.com/2079-4991/11/3/779 |
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