Influence of Temperature on Growth and Production of Pectenotoxin-2 by a Monoclonal Culture of Dinophysis caudata
The effects of temperature on growth and production of Lipophilic Toxins (LT) by a monoclonal culture of Dinophysis caudata was studied. The cell density of D. caudata increased significantly with increasing temperature, and was the highest under 27, 30, and 32.5 °C. Temperature affected the average...
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| Format: | Article |
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MDPI AG
2015-12-01
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| Series: | Marine Drugs |
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| Online Access: | http://www.mdpi.com/1660-3397/13/12/7061 |
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| author | Leila Basti Hajime Uchida Ryoji Matsushima Ryuichi Watanabe Toshiyuki Suzuki Toshifumi Yamatogi Satoshi Nagai |
| author_facet | Leila Basti Hajime Uchida Ryoji Matsushima Ryuichi Watanabe Toshiyuki Suzuki Toshifumi Yamatogi Satoshi Nagai |
| author_sort | Leila Basti |
| collection | DOAJ |
| description | The effects of temperature on growth and production of Lipophilic Toxins (LT) by a monoclonal culture of Dinophysis caudata was studied. The cell density of D. caudata increased significantly with increasing temperature, and was the highest under 27, 30, and 32.5 °C. Temperature affected the average specific growth rate (µ) during the exponential growth phase (EG), which increased from 15 °C to 30 °C, and then decreased at 32.5 °C. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) revealed that this strain of D. caudata produced only pectenotoxin-2 (PTX-2) whose concentration increased significantly with incubation period, except at 32.5 °C. It was significantly different between temperatures ≤18 °C, ≥21 °C, and 32.5 °C. The cellular toxin production (CTP, pg·cell−1·day−1) showed variation with growth phase and temperature, except at 32.5 °C. The average net toxin production (Rtox) was not affected by temperature. During EG, the average specific toxin production rate (µtox) increased significantly with increase in temperature, reaching a peak of 0.66 ± 0.01 day−1 at 30 °C, and then decreased. Over the entire growth span, µtox was significantly correlated to µ, and this correlation was most significant at 27 and 30 °C. During EG, µtox was affected by both temperature and growth. This study shows that temperature affects growth and toxin production of this strain of D. caudata during EG. In addition, a positive correlation was found between toxin production and growth. |
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| last_indexed | 2024-04-13T08:51:24Z |
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| series | Marine Drugs |
| spelling | doaj.art-c56e70d45648478ca3785a442e9cc5552022-12-22T02:53:29ZengMDPI AGMarine Drugs1660-33972015-12-0113127124713710.3390/md13127061md13127061Influence of Temperature on Growth and Production of Pectenotoxin-2 by a Monoclonal Culture of Dinophysis caudataLeila Basti0Hajime Uchida1Ryoji Matsushima2Ryuichi Watanabe3Toshiyuki Suzuki4Toshifumi Yamatogi5Satoshi Nagai6Department of Ocean Sciences, Tokyo University of Marine Science and Technology, Minato, Tokyo 108-8477, JapanNational research Institute of Fisheries Science, Fisheries Research Agency, Fukuura, Kanazawa, Yokohama, Kanagawa 236-8648, JapanNational research Institute of Fisheries Science, Fisheries Research Agency, Fukuura, Kanazawa, Yokohama, Kanagawa 236-8648, JapanNational research Institute of Fisheries Science, Fisheries Research Agency, Fukuura, Kanazawa, Yokohama, Kanagawa 236-8648, JapanNational research Institute of Fisheries Science, Fisheries Research Agency, Fukuura, Kanazawa, Yokohama, Kanagawa 236-8648, JapanNagasaki Prefectural Institute of Fisheries, 1551-4 Taira, Nagasaki, Nagasaki 851-2213, JapanNational research Institute of Fisheries Science, Fisheries Research Agency, Fukuura, Kanazawa, Yokohama, Kanagawa 236-8648, JapanThe effects of temperature on growth and production of Lipophilic Toxins (LT) by a monoclonal culture of Dinophysis caudata was studied. The cell density of D. caudata increased significantly with increasing temperature, and was the highest under 27, 30, and 32.5 °C. Temperature affected the average specific growth rate (µ) during the exponential growth phase (EG), which increased from 15 °C to 30 °C, and then decreased at 32.5 °C. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) revealed that this strain of D. caudata produced only pectenotoxin-2 (PTX-2) whose concentration increased significantly with incubation period, except at 32.5 °C. It was significantly different between temperatures ≤18 °C, ≥21 °C, and 32.5 °C. The cellular toxin production (CTP, pg·cell−1·day−1) showed variation with growth phase and temperature, except at 32.5 °C. The average net toxin production (Rtox) was not affected by temperature. During EG, the average specific toxin production rate (µtox) increased significantly with increase in temperature, reaching a peak of 0.66 ± 0.01 day−1 at 30 °C, and then decreased. Over the entire growth span, µtox was significantly correlated to µ, and this correlation was most significant at 27 and 30 °C. During EG, µtox was affected by both temperature and growth. This study shows that temperature affects growth and toxin production of this strain of D. caudata during EG. In addition, a positive correlation was found between toxin production and growth.http://www.mdpi.com/1660-3397/13/12/7061Dinophysis caudataculturegrowthpectenotoxin-2temperature |
| spellingShingle | Leila Basti Hajime Uchida Ryoji Matsushima Ryuichi Watanabe Toshiyuki Suzuki Toshifumi Yamatogi Satoshi Nagai Influence of Temperature on Growth and Production of Pectenotoxin-2 by a Monoclonal Culture of Dinophysis caudata Marine Drugs Dinophysis caudata culture growth pectenotoxin-2 temperature |
| title | Influence of Temperature on Growth and Production of Pectenotoxin-2 by a Monoclonal Culture of Dinophysis caudata |
| title_full | Influence of Temperature on Growth and Production of Pectenotoxin-2 by a Monoclonal Culture of Dinophysis caudata |
| title_fullStr | Influence of Temperature on Growth and Production of Pectenotoxin-2 by a Monoclonal Culture of Dinophysis caudata |
| title_full_unstemmed | Influence of Temperature on Growth and Production of Pectenotoxin-2 by a Monoclonal Culture of Dinophysis caudata |
| title_short | Influence of Temperature on Growth and Production of Pectenotoxin-2 by a Monoclonal Culture of Dinophysis caudata |
| title_sort | influence of temperature on growth and production of pectenotoxin 2 by a monoclonal culture of dinophysis caudata |
| topic | Dinophysis caudata culture growth pectenotoxin-2 temperature |
| url | http://www.mdpi.com/1660-3397/13/12/7061 |
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