Translational regulation of human embryonic kidney cell line HEK293
Objective To investigate the translational regulation of human embryonic kidney (HEK)293 cell line. Methods Polysome profiling coupled with Western blot was used to verify the translational activity of HEK293 cells. RPL10A and RPS17 RNA immunoprecipitation coupled with sequencing (RIP-seq) and bio...
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| Format: | Article |
| Language: | zho |
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Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.
2021-05-01
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| Series: | Jichu yixue yu linchuang |
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| Online Access: | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/a210081.pdf |
| _version_ | 1797365811890880512 |
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| author | LIU Man, REN Yue, GAO Yu-feng, WANG Fang, YU Jia |
| author_facet | LIU Man, REN Yue, GAO Yu-feng, WANG Fang, YU Jia |
| author_sort | LIU Man, REN Yue, GAO Yu-feng, WANG Fang, YU Jia |
| collection | DOAJ |
| description | Objective To investigate the translational regulation of human embryonic kidney (HEK)293 cell line. Methods Polysome profiling coupled with Western blot was used to verify the translational activity of HEK293 cells. RPL10A and RPS17 RNA immunoprecipitation coupled with sequencing (RIP-seq) and bioinformatic analysis were used to investigate the actively translating mRNAs. Results A total of 129 genes were found in the RNA immunoprecipitation experiments RPL10A and RPS17, among which 93.8% were protein-coding genes and enriched in RNA splicing, metabolism, MAPK signal pathway, chromatin localization and RNA transport. Conclusions A variety of molecules involved in HEK293 cells translation are obtained by RPL10A and RPS17 RIP-seq analysis. |
| first_indexed | 2024-03-08T16:54:15Z |
| format | Article |
| id | doaj.art-c56f37febf414b4a8c8298941694029d |
| institution | Directory Open Access Journal |
| issn | 1001-6325 |
| language | zho |
| last_indexed | 2024-03-08T16:54:15Z |
| publishDate | 2021-05-01 |
| publisher | Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College. |
| record_format | Article |
| series | Jichu yixue yu linchuang |
| spelling | doaj.art-c56f37febf414b4a8c8298941694029d2024-01-05T03:05:08ZzhoInstitute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.Jichu yixue yu linchuang1001-63252021-05-01415630635Translational regulation of human embryonic kidney cell line HEK293LIU Man, REN Yue, GAO Yu-feng, WANG Fang, YU Jia0State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences CAMS, School of Basic Medicine PUMC,Beijing 100005,ChinaObjective To investigate the translational regulation of human embryonic kidney (HEK)293 cell line. Methods Polysome profiling coupled with Western blot was used to verify the translational activity of HEK293 cells. RPL10A and RPS17 RNA immunoprecipitation coupled with sequencing (RIP-seq) and bioinformatic analysis were used to investigate the actively translating mRNAs. Results A total of 129 genes were found in the RNA immunoprecipitation experiments RPL10A and RPS17, among which 93.8% were protein-coding genes and enriched in RNA splicing, metabolism, MAPK signal pathway, chromatin localization and RNA transport. Conclusions A variety of molecules involved in HEK293 cells translation are obtained by RPL10A and RPS17 RIP-seq analysis.http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/a210081.pdfhuman embryonic kidney 293 cell line|polysome profiling|rna binding protein|immunoprecipitation |
| spellingShingle | LIU Man, REN Yue, GAO Yu-feng, WANG Fang, YU Jia Translational regulation of human embryonic kidney cell line HEK293 Jichu yixue yu linchuang human embryonic kidney 293 cell line|polysome profiling|rna binding protein|immunoprecipitation |
| title | Translational regulation of human embryonic kidney cell line HEK293 |
| title_full | Translational regulation of human embryonic kidney cell line HEK293 |
| title_fullStr | Translational regulation of human embryonic kidney cell line HEK293 |
| title_full_unstemmed | Translational regulation of human embryonic kidney cell line HEK293 |
| title_short | Translational regulation of human embryonic kidney cell line HEK293 |
| title_sort | translational regulation of human embryonic kidney cell line hek293 |
| topic | human embryonic kidney 293 cell line|polysome profiling|rna binding protein|immunoprecipitation |
| url | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/a210081.pdf |
| work_keys_str_mv | AT liumanrenyuegaoyufengwangfangyujia translationalregulationofhumanembryonickidneycelllinehek293 |