The Cellular Abundance of Chemoreceptors, Chemosensory Signaling Proteins, Sensor Histidine Kinases, and Solute Binding Proteins of <i>Pseudomonas aeruginosa</i> Provides Insight into Sensory Preferences and Signaling Mechanisms
Chemosensory pathways and two-component systems are important bacterial signal transduction systems. In the human pathogen <i>Pseudomonas aeruginosa,</i> these systems control many virulence traits. Previous studies showed that inorganic phosphate (Pi) deficiency induces virulence. We re...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-01-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/24/2/1363 |
| _version_ | 1797441375360253952 |
|---|---|
| author | Miguel A. Matilla Roberta Genova David Martín-Mora Sandra Maaβ Dörte Becher Tino Krell |
| author_facet | Miguel A. Matilla Roberta Genova David Martín-Mora Sandra Maaβ Dörte Becher Tino Krell |
| author_sort | Miguel A. Matilla |
| collection | DOAJ |
| description | Chemosensory pathways and two-component systems are important bacterial signal transduction systems. In the human pathogen <i>Pseudomonas aeruginosa,</i> these systems control many virulence traits. Previous studies showed that inorganic phosphate (Pi) deficiency induces virulence. We report here the abundance of chemosensory and two-component signaling proteins of <i>P. aeruginosa</i> grown in Pi deficient and sufficient media. The cellular abundance of chemoreceptors differed greatly, since a 2400-fold difference between the most and least abundant receptors was observed. For many chemoreceptors, their amount varied with the growth condition. The amount of chemoreceptors did not correlate with the magnitude of chemotaxis to their cognate chemoeffectors. Of the four chemosensory pathways, proteins of the Che chemotaxis pathway were most abundant and showed little variation in different growth conditions. The abundance of chemoreceptors and solute binding proteins indicates a sensing preference for amino acids and polyamines. There was an excess of response regulators over sensor histidine kinases in two-component systems. In contrast, ratios of the response regulators CheY and CheB to the histidine kinase CheA of the Che pathway were all below 1, indicative of different signaling mechanisms. This study will serve as a reference for exploring sensing preferences and signaling mechanisms of other bacteria. |
| first_indexed | 2024-03-09T12:22:09Z |
| format | Article |
| id | doaj.art-c56f41c2e39041929880cd3cd298dc11 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T12:22:09Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-c56f41c2e39041929880cd3cd298dc112023-11-30T22:39:15ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-01-01242136310.3390/ijms24021363The Cellular Abundance of Chemoreceptors, Chemosensory Signaling Proteins, Sensor Histidine Kinases, and Solute Binding Proteins of <i>Pseudomonas aeruginosa</i> Provides Insight into Sensory Preferences and Signaling MechanismsMiguel A. Matilla0Roberta Genova1David Martín-Mora2Sandra Maaβ3Dörte Becher4Tino Krell5Department of Biotechnology and Environmental Protection, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas, 18008 Granada, SpainDepartment of Biotechnology and Environmental Protection, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas, 18008 Granada, SpainDepartment of Biotechnology and Environmental Protection, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas, 18008 Granada, SpainDepartment of Microbial Proteomics, Institute of Microbiology, University of Greifswald, D-17489 Greifswald, GermanyDepartment of Microbial Proteomics, Institute of Microbiology, University of Greifswald, D-17489 Greifswald, GermanyDepartment of Biotechnology and Environmental Protection, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas, 18008 Granada, SpainChemosensory pathways and two-component systems are important bacterial signal transduction systems. In the human pathogen <i>Pseudomonas aeruginosa,</i> these systems control many virulence traits. Previous studies showed that inorganic phosphate (Pi) deficiency induces virulence. We report here the abundance of chemosensory and two-component signaling proteins of <i>P. aeruginosa</i> grown in Pi deficient and sufficient media. The cellular abundance of chemoreceptors differed greatly, since a 2400-fold difference between the most and least abundant receptors was observed. For many chemoreceptors, their amount varied with the growth condition. The amount of chemoreceptors did not correlate with the magnitude of chemotaxis to their cognate chemoeffectors. Of the four chemosensory pathways, proteins of the Che chemotaxis pathway were most abundant and showed little variation in different growth conditions. The abundance of chemoreceptors and solute binding proteins indicates a sensing preference for amino acids and polyamines. There was an excess of response regulators over sensor histidine kinases in two-component systems. In contrast, ratios of the response regulators CheY and CheB to the histidine kinase CheA of the Che pathway were all below 1, indicative of different signaling mechanisms. This study will serve as a reference for exploring sensing preferences and signaling mechanisms of other bacteria.https://www.mdpi.com/1422-0067/24/2/1363chemotaxischemoreceptorsensor histidine kinasessolute binding proteinchemosensory pathwayprotein abundance |
| spellingShingle | Miguel A. Matilla Roberta Genova David Martín-Mora Sandra Maaβ Dörte Becher Tino Krell The Cellular Abundance of Chemoreceptors, Chemosensory Signaling Proteins, Sensor Histidine Kinases, and Solute Binding Proteins of <i>Pseudomonas aeruginosa</i> Provides Insight into Sensory Preferences and Signaling Mechanisms International Journal of Molecular Sciences chemotaxis chemoreceptor sensor histidine kinases solute binding protein chemosensory pathway protein abundance |
| title | The Cellular Abundance of Chemoreceptors, Chemosensory Signaling Proteins, Sensor Histidine Kinases, and Solute Binding Proteins of <i>Pseudomonas aeruginosa</i> Provides Insight into Sensory Preferences and Signaling Mechanisms |
| title_full | The Cellular Abundance of Chemoreceptors, Chemosensory Signaling Proteins, Sensor Histidine Kinases, and Solute Binding Proteins of <i>Pseudomonas aeruginosa</i> Provides Insight into Sensory Preferences and Signaling Mechanisms |
| title_fullStr | The Cellular Abundance of Chemoreceptors, Chemosensory Signaling Proteins, Sensor Histidine Kinases, and Solute Binding Proteins of <i>Pseudomonas aeruginosa</i> Provides Insight into Sensory Preferences and Signaling Mechanisms |
| title_full_unstemmed | The Cellular Abundance of Chemoreceptors, Chemosensory Signaling Proteins, Sensor Histidine Kinases, and Solute Binding Proteins of <i>Pseudomonas aeruginosa</i> Provides Insight into Sensory Preferences and Signaling Mechanisms |
| title_short | The Cellular Abundance of Chemoreceptors, Chemosensory Signaling Proteins, Sensor Histidine Kinases, and Solute Binding Proteins of <i>Pseudomonas aeruginosa</i> Provides Insight into Sensory Preferences and Signaling Mechanisms |
| title_sort | cellular abundance of chemoreceptors chemosensory signaling proteins sensor histidine kinases and solute binding proteins of i pseudomonas aeruginosa i provides insight into sensory preferences and signaling mechanisms |
| topic | chemotaxis chemoreceptor sensor histidine kinases solute binding protein chemosensory pathway protein abundance |
| url | https://www.mdpi.com/1422-0067/24/2/1363 |
| work_keys_str_mv | AT miguelamatilla thecellularabundanceofchemoreceptorschemosensorysignalingproteinssensorhistidinekinasesandsolutebindingproteinsofipseudomonasaeruginosaiprovidesinsightintosensorypreferencesandsignalingmechanisms AT robertagenova thecellularabundanceofchemoreceptorschemosensorysignalingproteinssensorhistidinekinasesandsolutebindingproteinsofipseudomonasaeruginosaiprovidesinsightintosensorypreferencesandsignalingmechanisms AT davidmartinmora thecellularabundanceofchemoreceptorschemosensorysignalingproteinssensorhistidinekinasesandsolutebindingproteinsofipseudomonasaeruginosaiprovidesinsightintosensorypreferencesandsignalingmechanisms AT sandramaab thecellularabundanceofchemoreceptorschemosensorysignalingproteinssensorhistidinekinasesandsolutebindingproteinsofipseudomonasaeruginosaiprovidesinsightintosensorypreferencesandsignalingmechanisms AT dortebecher thecellularabundanceofchemoreceptorschemosensorysignalingproteinssensorhistidinekinasesandsolutebindingproteinsofipseudomonasaeruginosaiprovidesinsightintosensorypreferencesandsignalingmechanisms AT tinokrell thecellularabundanceofchemoreceptorschemosensorysignalingproteinssensorhistidinekinasesandsolutebindingproteinsofipseudomonasaeruginosaiprovidesinsightintosensorypreferencesandsignalingmechanisms AT miguelamatilla cellularabundanceofchemoreceptorschemosensorysignalingproteinssensorhistidinekinasesandsolutebindingproteinsofipseudomonasaeruginosaiprovidesinsightintosensorypreferencesandsignalingmechanisms AT robertagenova cellularabundanceofchemoreceptorschemosensorysignalingproteinssensorhistidinekinasesandsolutebindingproteinsofipseudomonasaeruginosaiprovidesinsightintosensorypreferencesandsignalingmechanisms AT davidmartinmora cellularabundanceofchemoreceptorschemosensorysignalingproteinssensorhistidinekinasesandsolutebindingproteinsofipseudomonasaeruginosaiprovidesinsightintosensorypreferencesandsignalingmechanisms AT sandramaab cellularabundanceofchemoreceptorschemosensorysignalingproteinssensorhistidinekinasesandsolutebindingproteinsofipseudomonasaeruginosaiprovidesinsightintosensorypreferencesandsignalingmechanisms AT dortebecher cellularabundanceofchemoreceptorschemosensorysignalingproteinssensorhistidinekinasesandsolutebindingproteinsofipseudomonasaeruginosaiprovidesinsightintosensorypreferencesandsignalingmechanisms AT tinokrell cellularabundanceofchemoreceptorschemosensorysignalingproteinssensorhistidinekinasesandsolutebindingproteinsofipseudomonasaeruginosaiprovidesinsightintosensorypreferencesandsignalingmechanisms |