Effect of Green Peas Protein Hydrolysate on Antihyperlipidemia and Antinephrotoxicity of Gentamicin-Induced Wistar Rats
Green peas protein hydrolysate is used in the treatment of kidney disease. Nephrotoxicity of gentamicin (GM) is known to be involved in kidney changes, dyslipidemia, and hematology. The aim of the present study is to evaluate the effects of three doses of green peas protein hydrolysate bromelain (GP...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2020-06-01
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| Series: | Acta Medica Iranica |
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| Online Access: | https://acta.tums.ac.ir/index.php/acta/article/view/7692 |
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| author | Meilinah Hidayat Sijani Prahastuti Widya Arina Ricky Febriansyah Sylvia S. Somya Audrey Aurelia Andreanus Andaja Soemardji Nova Suliska Afrilia N.Garmana Khomaini Hasan |
| author_facet | Meilinah Hidayat Sijani Prahastuti Widya Arina Ricky Febriansyah Sylvia S. Somya Audrey Aurelia Andreanus Andaja Soemardji Nova Suliska Afrilia N.Garmana Khomaini Hasan |
| author_sort | Meilinah Hidayat |
| collection | DOAJ |
| description | Green peas protein hydrolysate is used in the treatment of kidney disease. Nephrotoxicity of gentamicin (GM) is known to be involved in kidney changes, dyslipidemia, and hematology. The aim of the present study is to evaluate the effects of three doses of green peas protein hydrolysate bromelain (GPPHB) as antinephrotoxicity and antihyperlipidemia on gentamicin (GM)-induced Wistar rats as an effort to find an effective dose for kidney diseases therapy. Nephrotoxicity was induced in female Wistar rats through intraperitoneal administration of GM 80 mg/kg/day for seven days. Nine treatment groups (n=6) were monitored to determine the effects of the concurrent administration of green peas protein hydrolysate at a dose of 50, 100, and 200 mg/kg/day given with GM toward kidney damage using serum creatinine and blood urea nitrogen as indicators. The parameters for nephrotoxicity of GM were kidney organ index (OI), histopathological: cloudy swelling tubular degeneration, nucleus necrosis, and hyaline cast; hematology profiles; and for hyperlipidemia were total cholesterol, low-density lipoprotein (LDL), and triglyceride. After 28 days of treatments, GPPHB at the dose of 50 mg/kgBW has a good effect on kidney OI, whereas, at a dose of 200 mg/kgBW, GPPHB significantly protects rat kidneys from GM-induced histopathological changes. The GPPHB dose of 100 and 200 mg/kgBW significantly lowered the total cholesterol, LDL, and triglyceride levels but had no significant effect on hematology profiles. Three doses of GPPHB had antihyperlipidemia and antinephrotoxicity effects in GM-induced nephrotoxicity Wistar rats. |
| first_indexed | 2024-04-12T21:07:40Z |
| format | Article |
| id | doaj.art-c56fd0c03a314d41a80418990f9cdf11 |
| institution | Directory Open Access Journal |
| issn | 0044-6025 1735-9694 |
| language | English |
| last_indexed | 2024-04-12T21:07:40Z |
| publishDate | 2020-06-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Acta Medica Iranica |
| spelling | doaj.art-c56fd0c03a314d41a80418990f9cdf112022-12-22T03:16:40ZengTehran University of Medical SciencesActa Medica Iranica0044-60251735-96942020-06-01571210.18502/acta.v57i12.34647692Effect of Green Peas Protein Hydrolysate on Antihyperlipidemia and Antinephrotoxicity of Gentamicin-Induced Wistar RatsMeilinah Hidayat0Sijani Prahastuti1Widya Arina2Ricky Febriansyah3Sylvia S. Somya4Audrey Aurelia5Andreanus Andaja Soemardji6Nova Suliska7Afrilia N.Garmana8Khomaini Hasan9Faculty of Medicine, Universitas Kristen Maranatha, Bandung, Indonesia.Faculty of Medicine, Universitas Kristen Maranatha, Bandung, Indonesia.Faculty of Medicine, Universitas Kristen Maranatha, Bandung, Indonesia.Faculty of Medicine, Universitas Kristen Maranatha, Bandung, Indonesia.Faculty of Medicine, Universitas Kristen Maranatha, Bandung, Indonesia.Faculty of Medicine, Universitas Kristen Maranatha, Bandung, Indonesia.Pharmacy School, Institut Teknologi, Bandung, Indonesia.Pharmacy School, Institut Teknologi, Bandung, Indonesia.Pharmacy School, Institut Teknologi, Bandung, Indonesia.Faculty of Medicine, Universitas Jenderal Achmad Yani, Bandung, Indonesia.Green peas protein hydrolysate is used in the treatment of kidney disease. Nephrotoxicity of gentamicin (GM) is known to be involved in kidney changes, dyslipidemia, and hematology. The aim of the present study is to evaluate the effects of three doses of green peas protein hydrolysate bromelain (GPPHB) as antinephrotoxicity and antihyperlipidemia on gentamicin (GM)-induced Wistar rats as an effort to find an effective dose for kidney diseases therapy. Nephrotoxicity was induced in female Wistar rats through intraperitoneal administration of GM 80 mg/kg/day for seven days. Nine treatment groups (n=6) were monitored to determine the effects of the concurrent administration of green peas protein hydrolysate at a dose of 50, 100, and 200 mg/kg/day given with GM toward kidney damage using serum creatinine and blood urea nitrogen as indicators. The parameters for nephrotoxicity of GM were kidney organ index (OI), histopathological: cloudy swelling tubular degeneration, nucleus necrosis, and hyaline cast; hematology profiles; and for hyperlipidemia were total cholesterol, low-density lipoprotein (LDL), and triglyceride. After 28 days of treatments, GPPHB at the dose of 50 mg/kgBW has a good effect on kidney OI, whereas, at a dose of 200 mg/kgBW, GPPHB significantly protects rat kidneys from GM-induced histopathological changes. The GPPHB dose of 100 and 200 mg/kgBW significantly lowered the total cholesterol, LDL, and triglyceride levels but had no significant effect on hematology profiles. Three doses of GPPHB had antihyperlipidemia and antinephrotoxicity effects in GM-induced nephrotoxicity Wistar rats.https://acta.tums.ac.ir/index.php/acta/article/view/7692Green peas protein hydrolysateBromelainAntihyperlipidemiaHematologyHistopathological changes |
| spellingShingle | Meilinah Hidayat Sijani Prahastuti Widya Arina Ricky Febriansyah Sylvia S. Somya Audrey Aurelia Andreanus Andaja Soemardji Nova Suliska Afrilia N.Garmana Khomaini Hasan Effect of Green Peas Protein Hydrolysate on Antihyperlipidemia and Antinephrotoxicity of Gentamicin-Induced Wistar Rats Acta Medica Iranica Green peas protein hydrolysate Bromelain Antihyperlipidemia Hematology Histopathological changes |
| title | Effect of Green Peas Protein Hydrolysate on Antihyperlipidemia and Antinephrotoxicity of Gentamicin-Induced Wistar Rats |
| title_full | Effect of Green Peas Protein Hydrolysate on Antihyperlipidemia and Antinephrotoxicity of Gentamicin-Induced Wistar Rats |
| title_fullStr | Effect of Green Peas Protein Hydrolysate on Antihyperlipidemia and Antinephrotoxicity of Gentamicin-Induced Wistar Rats |
| title_full_unstemmed | Effect of Green Peas Protein Hydrolysate on Antihyperlipidemia and Antinephrotoxicity of Gentamicin-Induced Wistar Rats |
| title_short | Effect of Green Peas Protein Hydrolysate on Antihyperlipidemia and Antinephrotoxicity of Gentamicin-Induced Wistar Rats |
| title_sort | effect of green peas protein hydrolysate on antihyperlipidemia and antinephrotoxicity of gentamicin induced wistar rats |
| topic | Green peas protein hydrolysate Bromelain Antihyperlipidemia Hematology Histopathological changes |
| url | https://acta.tums.ac.ir/index.php/acta/article/view/7692 |
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