Electron Beam-Induced Immobilization of Laccase on Porous Supports for Waste Water Treatment Applications
The versatile oxidase enzyme laccase was immobilized on porous supports such as polymer membranes and cryogels with a view of using such biocatalysts in bioreactors aiming at the degradation of environmental pollutants in wastewater. Besides a large surface area for supporting the biocatalyst, the a...
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MDPI AG
2014-08-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/19/8/11860 |
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| author | Elham Jahangiri Senta Reichelt Isabell Thomas Kristin Hausmann Dietmar Schlosser Agnes Schulze |
| author_facet | Elham Jahangiri Senta Reichelt Isabell Thomas Kristin Hausmann Dietmar Schlosser Agnes Schulze |
| author_sort | Elham Jahangiri |
| collection | DOAJ |
| description | The versatile oxidase enzyme laccase was immobilized on porous supports such as polymer membranes and cryogels with a view of using such biocatalysts in bioreactors aiming at the degradation of environmental pollutants in wastewater. Besides a large surface area for supporting the biocatalyst, the aforementioned porous systems also offer the possibility for simultaneous filtration applications in wastewater treatment. Herein a “green” water-based, initiator-free, and straightforward route to highly reactive membrane and cryogel-based bioreactors is presented, where laccase was immobilized onto the porous polymer supports using a water-based electron beam-initiated grafting reaction. In a second approach, the laccase redox mediators 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) and syringaldehyde were cross-linked instead of the enzyme via electron irradiation in a frozen aqueous poly(acrylate) mixture in a one pot set-up, yielding a mechanical stable macroporous cryogel with interconnected pores ranging from 10 to 50 µm in size. The membranes as well as the cryogels were characterized regarding their morphology, chemical composition, and catalytic activity. The reactivity towards waste- water pollutants was demonstrated by the degradation of the model compound bisphenol A (BPA). Both membrane- and cryogel-immobilized laccase remained highly active after electron beam irradiation. Apparent specific BPA removal rates were higher for cryogel- than for membrane-immobilized and free laccase, whereas membrane-immobilized laccase was more stable with respect to maintenance of enzymatic activity and prevention of enzyme leakage from the carrier than cryogel-immobilized laccase. Cryogel-immobilized redox mediators remained functional in accelerating the laccase-catalyzed BPA degradation, and especially ABTS was found to act more efficiently in immobilized than in freely dissolved state. |
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| issn | 1420-3049 |
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| last_indexed | 2024-12-14T13:29:12Z |
| publishDate | 2014-08-01 |
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| series | Molecules |
| spelling | doaj.art-c5706d8a6a2647498d65f66e1fe149592022-12-21T22:59:45ZengMDPI AGMolecules1420-30492014-08-01198118601188210.3390/molecules190811860molecules190811860Electron Beam-Induced Immobilization of Laccase on Porous Supports for Waste Water Treatment ApplicationsElham Jahangiri0Senta Reichelt1Isabell Thomas2Kristin Hausmann3Dietmar Schlosser4Agnes Schulze5Helmholtz Centre for Environmental Research, Permoserstr. 15, D-04318 Leipzig, GermanyLeibniz Institute of Surface Modification, Permoserstr. 15, D-04318 Leipzig, GermanyLeibniz Institute of Surface Modification, Permoserstr. 15, D-04318 Leipzig, GermanyLeibniz Institute of Surface Modification, Permoserstr. 15, D-04318 Leipzig, GermanyHelmholtz Centre for Environmental Research, Permoserstr. 15, D-04318 Leipzig, GermanyLeibniz Institute of Surface Modification, Permoserstr. 15, D-04318 Leipzig, GermanyThe versatile oxidase enzyme laccase was immobilized on porous supports such as polymer membranes and cryogels with a view of using such biocatalysts in bioreactors aiming at the degradation of environmental pollutants in wastewater. Besides a large surface area for supporting the biocatalyst, the aforementioned porous systems also offer the possibility for simultaneous filtration applications in wastewater treatment. Herein a “green” water-based, initiator-free, and straightforward route to highly reactive membrane and cryogel-based bioreactors is presented, where laccase was immobilized onto the porous polymer supports using a water-based electron beam-initiated grafting reaction. In a second approach, the laccase redox mediators 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) and syringaldehyde were cross-linked instead of the enzyme via electron irradiation in a frozen aqueous poly(acrylate) mixture in a one pot set-up, yielding a mechanical stable macroporous cryogel with interconnected pores ranging from 10 to 50 µm in size. The membranes as well as the cryogels were characterized regarding their morphology, chemical composition, and catalytic activity. The reactivity towards waste- water pollutants was demonstrated by the degradation of the model compound bisphenol A (BPA). Both membrane- and cryogel-immobilized laccase remained highly active after electron beam irradiation. Apparent specific BPA removal rates were higher for cryogel- than for membrane-immobilized and free laccase, whereas membrane-immobilized laccase was more stable with respect to maintenance of enzymatic activity and prevention of enzyme leakage from the carrier than cryogel-immobilized laccase. Cryogel-immobilized redox mediators remained functional in accelerating the laccase-catalyzed BPA degradation, and especially ABTS was found to act more efficiently in immobilized than in freely dissolved state.http://www.mdpi.com/1420-3049/19/8/11860cryogelsmembraneslaccase immobilizationelectron beam irradiationbioreactordegradation of pollutantsredox mediator immobilization |
| spellingShingle | Elham Jahangiri Senta Reichelt Isabell Thomas Kristin Hausmann Dietmar Schlosser Agnes Schulze Electron Beam-Induced Immobilization of Laccase on Porous Supports for Waste Water Treatment Applications Molecules cryogels membranes laccase immobilization electron beam irradiation bioreactor degradation of pollutants redox mediator immobilization |
| title | Electron Beam-Induced Immobilization of Laccase on Porous Supports for Waste Water Treatment Applications |
| title_full | Electron Beam-Induced Immobilization of Laccase on Porous Supports for Waste Water Treatment Applications |
| title_fullStr | Electron Beam-Induced Immobilization of Laccase on Porous Supports for Waste Water Treatment Applications |
| title_full_unstemmed | Electron Beam-Induced Immobilization of Laccase on Porous Supports for Waste Water Treatment Applications |
| title_short | Electron Beam-Induced Immobilization of Laccase on Porous Supports for Waste Water Treatment Applications |
| title_sort | electron beam induced immobilization of laccase on porous supports for waste water treatment applications |
| topic | cryogels membranes laccase immobilization electron beam irradiation bioreactor degradation of pollutants redox mediator immobilization |
| url | http://www.mdpi.com/1420-3049/19/8/11860 |
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