Hepatoprotective Effect of the Aqueous Extract of Simarouba amara Aublet (Simaroubaceae) Stem Bark against Carbon Tetrachloride (CCl4)-Induced Hepatic Damage in Rats
Simarouba amara stem bark decoction has been traditionally used in Brazil to treat malaria, inflammation, fever, abdominal pain, diarrhea, wounds and as a tonic. In this study, we investigate the hepatoprotective effects of the aqueous extract of S. amara stem bark (SAAE) on CCl4-induced hepatic dam...
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MDPI AG
2014-10-01
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| author | Hélida M. L. Maranhão Carlos F. B. Vasconcelos Larissa A. Rolim Pedro J. Rolim Neto Jacinto da C. Silva Neto Reginaldo C. da Silva Filho Mariana P. Fernandes João H. Costa-Silva Alice V. Araújo Almir G. Wanderley |
| author_facet | Hélida M. L. Maranhão Carlos F. B. Vasconcelos Larissa A. Rolim Pedro J. Rolim Neto Jacinto da C. Silva Neto Reginaldo C. da Silva Filho Mariana P. Fernandes João H. Costa-Silva Alice V. Araújo Almir G. Wanderley |
| author_sort | Hélida M. L. Maranhão |
| collection | DOAJ |
| description | Simarouba amara stem bark decoction has been traditionally used in Brazil to treat malaria, inflammation, fever, abdominal pain, diarrhea, wounds and as a tonic. In this study, we investigate the hepatoprotective effects of the aqueous extract of S. amara stem bark (SAAE) on CCl4-induced hepatic damage in rats. SAAE was evaluated by high performance liquid chromatography. The animals were divided into six groups (n = 6/group). Groups I (vehicle—corn oil), II (control-CCl4), III, IV, V and VI were pretreated during 10 consecutive days, once a day p.o, with Legalon® 50 mg/kg b.w, SAAE at doses 100, 250 and 500 mg/kg b.w, respectively. The hepatotoxicity was induced on 11th day with 2 mL/kg of 20% CCl4 solution. 24 h after injury, the blood samples were collected and their livers were removed to biochemical and immunohistochemical analyzes. The SAAE decreased the levels of liver markers and lipid peroxidation in all doses and increased the catalase levels at doses 250 and 500 mg/kg. Immunohistochemical results suggested hepatocyte proliferation in all doses. These results may be related to catechins present in SAAE. Thus, SAAE prevented the oxidative damage at the same time that increased regenerative and reparative capacities of the liver. |
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| spelling | doaj.art-c571a470ee0545a3b2f26f20c839dc1a2022-12-21T21:43:20ZengMDPI AGMolecules1420-30492014-10-011911177351774610.3390/molecules191117735molecules191117735Hepatoprotective Effect of the Aqueous Extract of Simarouba amara Aublet (Simaroubaceae) Stem Bark against Carbon Tetrachloride (CCl4)-Induced Hepatic Damage in RatsHélida M. L. Maranhão0Carlos F. B. Vasconcelos1Larissa A. Rolim2Pedro J. Rolim Neto3Jacinto da C. Silva Neto4Reginaldo C. da Silva Filho5Mariana P. Fernandes6João H. Costa-Silva7Alice V. Araújo8Almir G. Wanderley9Department of Pharmaceutical Sciences, Universidade Federal de Pernambuco, Recife 50740-521, BrazilDepartment of Pharmaceutical Sciences, Universidade Federal de Pernambuco, Recife 50740-521, BrazilDepartment of Pharmaceutical Sciences, Universidade Federal de Pernambuco, Recife 50740-521, BrazilDepartment of Pharmaceutical Sciences, Universidade Federal de Pernambuco, Recife 50740-521, BrazilDepartment of Histology and Embriology, Universidade Federal de Pernambuco, Recife 50670-901, BrazilDepartment of Physical Education and Sport Science, Universidade Federal de Pernambuco, Vitória de Santo Antão 55608-680, BrazilDepartment of Physical Education and Sport Science, Universidade Federal de Pernambuco, Vitória de Santo Antão 55608-680, BrazilDepartment of Physical Education and Sport Science, Universidade Federal de Pernambuco, Vitória de Santo Antão 55608-680, BrazilDepartment of Nutrition, Universidade Federal de Pernambuco, Vitória de Santo Antão 55608-680, BrazilDepartment of Pharmaceutical Sciences, Universidade Federal de Pernambuco, Recife 50740-521, BrazilSimarouba amara stem bark decoction has been traditionally used in Brazil to treat malaria, inflammation, fever, abdominal pain, diarrhea, wounds and as a tonic. In this study, we investigate the hepatoprotective effects of the aqueous extract of S. amara stem bark (SAAE) on CCl4-induced hepatic damage in rats. SAAE was evaluated by high performance liquid chromatography. The animals were divided into six groups (n = 6/group). Groups I (vehicle—corn oil), II (control-CCl4), III, IV, V and VI were pretreated during 10 consecutive days, once a day p.o, with Legalon® 50 mg/kg b.w, SAAE at doses 100, 250 and 500 mg/kg b.w, respectively. The hepatotoxicity was induced on 11th day with 2 mL/kg of 20% CCl4 solution. 24 h after injury, the blood samples were collected and their livers were removed to biochemical and immunohistochemical analyzes. The SAAE decreased the levels of liver markers and lipid peroxidation in all doses and increased the catalase levels at doses 250 and 500 mg/kg. Immunohistochemical results suggested hepatocyte proliferation in all doses. These results may be related to catechins present in SAAE. Thus, SAAE prevented the oxidative damage at the same time that increased regenerative and reparative capacities of the liver.http://www.mdpi.com/1420-3049/19/11/17735SimaroubaceaeSimarouba amaracarbon tetrachloridecatechinhepatoprotectionantioxidant |
| spellingShingle | Hélida M. L. Maranhão Carlos F. B. Vasconcelos Larissa A. Rolim Pedro J. Rolim Neto Jacinto da C. Silva Neto Reginaldo C. da Silva Filho Mariana P. Fernandes João H. Costa-Silva Alice V. Araújo Almir G. Wanderley Hepatoprotective Effect of the Aqueous Extract of Simarouba amara Aublet (Simaroubaceae) Stem Bark against Carbon Tetrachloride (CCl4)-Induced Hepatic Damage in Rats Molecules Simaroubaceae Simarouba amara carbon tetrachloride catechin hepatoprotection antioxidant |
| title | Hepatoprotective Effect of the Aqueous Extract of Simarouba amara Aublet (Simaroubaceae) Stem Bark against Carbon Tetrachloride (CCl4)-Induced Hepatic Damage in Rats |
| title_full | Hepatoprotective Effect of the Aqueous Extract of Simarouba amara Aublet (Simaroubaceae) Stem Bark against Carbon Tetrachloride (CCl4)-Induced Hepatic Damage in Rats |
| title_fullStr | Hepatoprotective Effect of the Aqueous Extract of Simarouba amara Aublet (Simaroubaceae) Stem Bark against Carbon Tetrachloride (CCl4)-Induced Hepatic Damage in Rats |
| title_full_unstemmed | Hepatoprotective Effect of the Aqueous Extract of Simarouba amara Aublet (Simaroubaceae) Stem Bark against Carbon Tetrachloride (CCl4)-Induced Hepatic Damage in Rats |
| title_short | Hepatoprotective Effect of the Aqueous Extract of Simarouba amara Aublet (Simaroubaceae) Stem Bark against Carbon Tetrachloride (CCl4)-Induced Hepatic Damage in Rats |
| title_sort | hepatoprotective effect of the aqueous extract of simarouba amara aublet simaroubaceae stem bark against carbon tetrachloride ccl4 induced hepatic damage in rats |
| topic | Simaroubaceae Simarouba amara carbon tetrachloride catechin hepatoprotection antioxidant |
| url | http://www.mdpi.com/1420-3049/19/11/17735 |
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