Systemic Resistance in Arabidopsis Induced by Rhizobacteria Requires Ethylene-Dependent Signaling at the Site of Application
Root colonization of Arabidopsis thaliana by the nonpathogenic, rhizosphere-colonizing, biocontrol bacterium Pseudomonas fluorescens WCS417r has been shown to elicit induced systemic resistance (ISR) against Pseudomonas syringae pv. tomato (Pst). The ISR response differs from the pathogen-inducible...
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
1999-08-01
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| Series: | Molecular Plant-Microbe Interactions |
| Subjects: | |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI.1999.12.8.720 |
| _version_ | 1818915090843303936 |
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| author | Marga Knoester Corné M. J. Pieterse John F. Bol Leendert C. Van Loon |
| author_facet | Marga Knoester Corné M. J. Pieterse John F. Bol Leendert C. Van Loon |
| author_sort | Marga Knoester |
| collection | DOAJ |
| description | Root colonization of Arabidopsis thaliana by the nonpathogenic, rhizosphere-colonizing, biocontrol bacterium Pseudomonas fluorescens WCS417r has been shown to elicit induced systemic resistance (ISR) against Pseudomonas syringae pv. tomato (Pst). The ISR response differs from the pathogen-inducible systemic acquired resistance (SAR) response in that ISR is independent of salicylic acid and not associated with pathogenesis-related proteins. Several ethylene-response mutants were tested and showed essentially normal symptoms of Pst infection. ISR was abolished in the ethylene-insensitive mutant etr1-1, whereas SAR was unaffected. Similar results were obtained with the ethylene-insensitive mutants ein2 through ein7, indicating that the expression of ISR requires the complete signal-transduction pathway of ethylene known so far. The induction of ISR by WCS417r was not accompanied by increased ethylene production in roots or leaves, nor by increases in the expression of the genes encoding the ethylene biosynthetic enzymes 1-aminocyclopropane-1-carboxylic (ACC) synthase and ACC oxidase. The eir1 mutant, displaying ethylene insensitivity in the roots only, did not express ISR upon application of WCS417r to the roots, but did exhibit ISR when the inducing bacteria were infiltrated into the leaves. These results demonstrate that, for the induction of ISR, ethylene responsiveness is required at the site of application of inducing rhizobacteria. |
| first_indexed | 2024-12-19T23:56:45Z |
| format | Article |
| id | doaj.art-c575499f2fb342088b5c4b028d00137d |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-12-19T23:56:45Z |
| publishDate | 1999-08-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-c575499f2fb342088b5c4b028d00137d2022-12-21T20:00:59ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77061999-08-0112872072710.1094/MPMI.1999.12.8.720Systemic Resistance in Arabidopsis Induced by Rhizobacteria Requires Ethylene-Dependent Signaling at the Site of ApplicationMarga KnoesterCorné M. J. PieterseJohn F. BolLeendert C. Van LoonRoot colonization of Arabidopsis thaliana by the nonpathogenic, rhizosphere-colonizing, biocontrol bacterium Pseudomonas fluorescens WCS417r has been shown to elicit induced systemic resistance (ISR) against Pseudomonas syringae pv. tomato (Pst). The ISR response differs from the pathogen-inducible systemic acquired resistance (SAR) response in that ISR is independent of salicylic acid and not associated with pathogenesis-related proteins. Several ethylene-response mutants were tested and showed essentially normal symptoms of Pst infection. ISR was abolished in the ethylene-insensitive mutant etr1-1, whereas SAR was unaffected. Similar results were obtained with the ethylene-insensitive mutants ein2 through ein7, indicating that the expression of ISR requires the complete signal-transduction pathway of ethylene known so far. The induction of ISR by WCS417r was not accompanied by increased ethylene production in roots or leaves, nor by increases in the expression of the genes encoding the ethylene biosynthetic enzymes 1-aminocyclopropane-1-carboxylic (ACC) synthase and ACC oxidase. The eir1 mutant, displaying ethylene insensitivity in the roots only, did not express ISR upon application of WCS417r to the roots, but did exhibit ISR when the inducing bacteria were infiltrated into the leaves. These results demonstrate that, for the induction of ISR, ethylene responsiveness is required at the site of application of inducing rhizobacteria.https://apsjournals.apsnet.org/doi/10.1094/MPMI.1999.12.8.720bacterial speck diseaseethylene response mutants |
| spellingShingle | Marga Knoester Corné M. J. Pieterse John F. Bol Leendert C. Van Loon Systemic Resistance in Arabidopsis Induced by Rhizobacteria Requires Ethylene-Dependent Signaling at the Site of Application Molecular Plant-Microbe Interactions bacterial speck disease ethylene response mutants |
| title | Systemic Resistance in Arabidopsis Induced by Rhizobacteria Requires Ethylene-Dependent Signaling at the Site of Application |
| title_full | Systemic Resistance in Arabidopsis Induced by Rhizobacteria Requires Ethylene-Dependent Signaling at the Site of Application |
| title_fullStr | Systemic Resistance in Arabidopsis Induced by Rhizobacteria Requires Ethylene-Dependent Signaling at the Site of Application |
| title_full_unstemmed | Systemic Resistance in Arabidopsis Induced by Rhizobacteria Requires Ethylene-Dependent Signaling at the Site of Application |
| title_short | Systemic Resistance in Arabidopsis Induced by Rhizobacteria Requires Ethylene-Dependent Signaling at the Site of Application |
| title_sort | systemic resistance in arabidopsis induced by rhizobacteria requires ethylene dependent signaling at the site of application |
| topic | bacterial speck disease ethylene response mutants |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI.1999.12.8.720 |
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