Proteins and Signaling Pathways Response to Dry Needling Combined with Static Stretching Treatment for Chronic Myofascial Pain in a RAT Model: An Explorative Proteomic Study
A quantitative proteomic analysis of the response to dry needling combined with static stretching treatment was performed in a rat model of active myofascial trigger points (MTrPs). 36 rats were divided into a model group (MG), a stretching group (SG) and a dry needling combined with stretching grou...
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MDPI AG
2019-01-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/20/3/564 |
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| author | Lihui Li Qiangmin Huang Marco Barbero Lin Liu Thitham Nguyen Anle Xu Lijuan Ji |
| author_facet | Lihui Li Qiangmin Huang Marco Barbero Lin Liu Thitham Nguyen Anle Xu Lijuan Ji |
| author_sort | Lihui Li |
| collection | DOAJ |
| description | A quantitative proteomic analysis of the response to dry needling combined with static stretching treatment was performed in a rat model of active myofascial trigger points (MTrPs). 36 rats were divided into a model group (MG), a stretching group (SG) and a dry needling combined with stretching group (SDG). We performed three biological replicates to compare large-scale differential protein expression between groups by tandem mass tag (TMT) labeling technology based on nanoscale liquid chromatography mass spectrometry analysis (LC⁻MS/MS). Hierarchical clustering, Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment and protein-protein interaction network analyses were performed for the general characterization of overall enriched proteins. For validation of the results of TMT, the candidate proteins were verified by parallel reaction monitoring (PRM) analysis. 285 differentially expressed proteins between groups were identified and quantified. Tight junction pathway played a dominant role in dry needling combined with static stretching treatment for the rat model of active MTrPs. Three candidate proteins, namely actinin alpha 3, calsequestrin-1 and parvalbumin alpha, were further validated, consistent with the results of LC⁻MS/MS. This is the first proteomics-based study to report the therapeutic mechanism underlying dry needling and static stretching treatment for MTrPs. Further functional verification of the potential signaling pathways and the enriched proteins is warranted. |
| first_indexed | 2024-04-11T23:14:43Z |
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| institution | Directory Open Access Journal |
| issn | 1422-0067 |
| language | English |
| last_indexed | 2024-04-11T23:14:43Z |
| publishDate | 2019-01-01 |
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| spelling | doaj.art-c57712dc0a694263825b264cac3aa7ed2022-12-22T03:57:40ZengMDPI AGInternational Journal of Molecular Sciences1422-00672019-01-0120356410.3390/ijms20030564ijms20030564Proteins and Signaling Pathways Response to Dry Needling Combined with Static Stretching Treatment for Chronic Myofascial Pain in a RAT Model: An Explorative Proteomic StudyLihui Li0Qiangmin Huang1Marco Barbero2Lin Liu3Thitham Nguyen4Anle Xu5Lijuan Ji6Department of Sport Medicine and Rehabilitation Center, Shanghai University of Sport, Shanghai 200438, ChinaDepartment of Sport Medicine and Rehabilitation Center, Shanghai University of Sport, Shanghai 200438, ChinaRehabilitation Research Laboratory 2rLab, Department of Business Economics Health and Social Care, University of Applied Sciences and Arts of Southern Switzerland, 6928 Manno, SwitzerlandSport and Health Science Department, Nanjing Sport Institute, Nanjing 210014, ChinaFaculty of Sport Science, Ton Duc Thang University, Ho Chi Minh City 71000, Viet NamDepartment of Sport Medicine and Rehabilitation Center, Shanghai University of Sport, Shanghai 200438, ChinaDepartment of Sport Medicine and Rehabilitation Center, Shanghai University of Sport, Shanghai 200438, ChinaA quantitative proteomic analysis of the response to dry needling combined with static stretching treatment was performed in a rat model of active myofascial trigger points (MTrPs). 36 rats were divided into a model group (MG), a stretching group (SG) and a dry needling combined with stretching group (SDG). We performed three biological replicates to compare large-scale differential protein expression between groups by tandem mass tag (TMT) labeling technology based on nanoscale liquid chromatography mass spectrometry analysis (LC⁻MS/MS). Hierarchical clustering, Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment and protein-protein interaction network analyses were performed for the general characterization of overall enriched proteins. For validation of the results of TMT, the candidate proteins were verified by parallel reaction monitoring (PRM) analysis. 285 differentially expressed proteins between groups were identified and quantified. Tight junction pathway played a dominant role in dry needling combined with static stretching treatment for the rat model of active MTrPs. Three candidate proteins, namely actinin alpha 3, calsequestrin-1 and parvalbumin alpha, were further validated, consistent with the results of LC⁻MS/MS. This is the first proteomics-based study to report the therapeutic mechanism underlying dry needling and static stretching treatment for MTrPs. Further functional verification of the potential signaling pathways and the enriched proteins is warranted.https://www.mdpi.com/1422-0067/20/3/564myofascial trigger pointsproteomicchronic myofascial paindry needlingstretchingmass spectrometrytandem mass tagactinin alpha 3calsequestrin-1parvalbumin alpha |
| spellingShingle | Lihui Li Qiangmin Huang Marco Barbero Lin Liu Thitham Nguyen Anle Xu Lijuan Ji Proteins and Signaling Pathways Response to Dry Needling Combined with Static Stretching Treatment for Chronic Myofascial Pain in a RAT Model: An Explorative Proteomic Study International Journal of Molecular Sciences myofascial trigger points proteomic chronic myofascial pain dry needling stretching mass spectrometry tandem mass tag actinin alpha 3 calsequestrin-1 parvalbumin alpha |
| title | Proteins and Signaling Pathways Response to Dry Needling Combined with Static Stretching Treatment for Chronic Myofascial Pain in a RAT Model: An Explorative Proteomic Study |
| title_full | Proteins and Signaling Pathways Response to Dry Needling Combined with Static Stretching Treatment for Chronic Myofascial Pain in a RAT Model: An Explorative Proteomic Study |
| title_fullStr | Proteins and Signaling Pathways Response to Dry Needling Combined with Static Stretching Treatment for Chronic Myofascial Pain in a RAT Model: An Explorative Proteomic Study |
| title_full_unstemmed | Proteins and Signaling Pathways Response to Dry Needling Combined with Static Stretching Treatment for Chronic Myofascial Pain in a RAT Model: An Explorative Proteomic Study |
| title_short | Proteins and Signaling Pathways Response to Dry Needling Combined with Static Stretching Treatment for Chronic Myofascial Pain in a RAT Model: An Explorative Proteomic Study |
| title_sort | proteins and signaling pathways response to dry needling combined with static stretching treatment for chronic myofascial pain in a rat model an explorative proteomic study |
| topic | myofascial trigger points proteomic chronic myofascial pain dry needling stretching mass spectrometry tandem mass tag actinin alpha 3 calsequestrin-1 parvalbumin alpha |
| url | https://www.mdpi.com/1422-0067/20/3/564 |
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