Generation of a felinized swine endothelial cell line by expression of feline decay-accelerating factor.
Embryonic stem cell research has facilitated the generation of many cell types for the production of tissues and organs for both humans and companion animals. Because ≥30% of pet cats suffer from chronic kidney disease (CKD), xenotransplantation between pigs and cats has been studied. For a successf...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4324824?pdf=render |
| _version_ | 1818277088326582272 |
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| author | Luna Izuhara Norifumi Tatsumi Shuji Miyagawa Satomi Iwai Masahito Watanabe Shuichiro Yamanaka Yuichi Katsuoka Hiroshi Nagashima Hirotaka J Okano Takashi Yokoo |
| author_facet | Luna Izuhara Norifumi Tatsumi Shuji Miyagawa Satomi Iwai Masahito Watanabe Shuichiro Yamanaka Yuichi Katsuoka Hiroshi Nagashima Hirotaka J Okano Takashi Yokoo |
| author_sort | Luna Izuhara |
| collection | DOAJ |
| description | Embryonic stem cell research has facilitated the generation of many cell types for the production of tissues and organs for both humans and companion animals. Because ≥30% of pet cats suffer from chronic kidney disease (CKD), xenotransplantation between pigs and cats has been studied. For a successful pig to cat xenotransplant, the immune reaction must be overcome, especially hyperacute rejection. In this study, we isolated the gene for feline decay-accelerating factor (fDAF), an inhibitor of complement proteins, and transfected a swine endothelial cell line with fDAF to "felinize" the pig cells. These fDAF-expressing cells were resistant to feline serum containing anti-pig antibodies, suggesting that felinized pig cells were resistant to hyperacute rejection. Our results suggest that a "felinized" pig kidney can be generated for the treatment of CKD in cats in the future. |
| first_indexed | 2024-12-12T22:55:59Z |
| format | Article |
| id | doaj.art-c5783eb158f041c08e0f76f6d740d199 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-12T22:55:59Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-c5783eb158f041c08e0f76f6d740d1992022-12-22T00:08:57ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01102e011768210.1371/journal.pone.0117682Generation of a felinized swine endothelial cell line by expression of feline decay-accelerating factor.Luna IzuharaNorifumi TatsumiShuji MiyagawaSatomi IwaiMasahito WatanabeShuichiro YamanakaYuichi KatsuokaHiroshi NagashimaHirotaka J OkanoTakashi YokooEmbryonic stem cell research has facilitated the generation of many cell types for the production of tissues and organs for both humans and companion animals. Because ≥30% of pet cats suffer from chronic kidney disease (CKD), xenotransplantation between pigs and cats has been studied. For a successful pig to cat xenotransplant, the immune reaction must be overcome, especially hyperacute rejection. In this study, we isolated the gene for feline decay-accelerating factor (fDAF), an inhibitor of complement proteins, and transfected a swine endothelial cell line with fDAF to "felinize" the pig cells. These fDAF-expressing cells were resistant to feline serum containing anti-pig antibodies, suggesting that felinized pig cells were resistant to hyperacute rejection. Our results suggest that a "felinized" pig kidney can be generated for the treatment of CKD in cats in the future.http://europepmc.org/articles/PMC4324824?pdf=render |
| spellingShingle | Luna Izuhara Norifumi Tatsumi Shuji Miyagawa Satomi Iwai Masahito Watanabe Shuichiro Yamanaka Yuichi Katsuoka Hiroshi Nagashima Hirotaka J Okano Takashi Yokoo Generation of a felinized swine endothelial cell line by expression of feline decay-accelerating factor. PLoS ONE |
| title | Generation of a felinized swine endothelial cell line by expression of feline decay-accelerating factor. |
| title_full | Generation of a felinized swine endothelial cell line by expression of feline decay-accelerating factor. |
| title_fullStr | Generation of a felinized swine endothelial cell line by expression of feline decay-accelerating factor. |
| title_full_unstemmed | Generation of a felinized swine endothelial cell line by expression of feline decay-accelerating factor. |
| title_short | Generation of a felinized swine endothelial cell line by expression of feline decay-accelerating factor. |
| title_sort | generation of a felinized swine endothelial cell line by expression of feline decay accelerating factor |
| url | http://europepmc.org/articles/PMC4324824?pdf=render |
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