Polymorphism in the Plasmodium vivax msp 3: gene in field samples from Tierralta, Colombia
We evaluated the Plasmodium vivax polymorphism by studying the Pvmsp-3 gene's polymorphic region by PCR-RFLP in 55 samples from patients living in Tierralta, Colombia. Three different sizes of the Pvmsp-3 gene were found, type A (1,900 bp), type B (1,500 bp) and type C (1,100 bp); most of the s...
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
2008-08-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762008000500015 |
| _version_ | 1797712697182126080 |
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| author | Fabio Aníbal Cristiano Manuel Alberto Pérez Rubén Santiago Nicholls Angela Patricia Guerra |
| author_facet | Fabio Aníbal Cristiano Manuel Alberto Pérez Rubén Santiago Nicholls Angela Patricia Guerra |
| author_sort | Fabio Aníbal Cristiano |
| collection | DOAJ |
| description | We evaluated the Plasmodium vivax polymorphism by studying the Pvmsp-3 gene's polymorphic region by PCR-RFLP in 55 samples from patients living in Tierralta, Colombia. Three different sizes of the Pvmsp-3 gene were found, type A (1,900 bp), type B (1,500 bp) and type C (1,100 bp); most of the samples were type A (96.4 %). The Pvmsp-3 gene exhibited high polymorphism. Seven restriction patterns were found when using Alu I, and nine were found with Hha I; 12 different alleles were obtained when these patterns were combined. The findings suggest that this gene could be used in Colombia as a molecular epidemiologic marker for genotyping P. vivax. |
| first_indexed | 2024-03-12T07:25:32Z |
| format | Article |
| id | doaj.art-c57ac5a055994b16ad569c84ec273ffe |
| institution | Directory Open Access Journal |
| issn | 0074-0276 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T07:25:32Z |
| publishDate | 2008-08-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-c57ac5a055994b16ad569c84ec273ffe2023-09-02T22:11:04ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz0074-02761678-80602008-08-01103549349610.1590/S0074-02762008000500015Polymorphism in the Plasmodium vivax msp 3: gene in field samples from Tierralta, ColombiaFabio Aníbal CristianoManuel Alberto PérezRubén Santiago NichollsAngela Patricia GuerraWe evaluated the Plasmodium vivax polymorphism by studying the Pvmsp-3 gene's polymorphic region by PCR-RFLP in 55 samples from patients living in Tierralta, Colombia. Three different sizes of the Pvmsp-3 gene were found, type A (1,900 bp), type B (1,500 bp) and type C (1,100 bp); most of the samples were type A (96.4 %). The Pvmsp-3 gene exhibited high polymorphism. Seven restriction patterns were found when using Alu I, and nine were found with Hha I; 12 different alleles were obtained when these patterns were combined. The findings suggest that this gene could be used in Colombia as a molecular epidemiologic marker for genotyping P. vivax.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762008000500015Plasmodium vivaxColombiagenetic polymorphism |
| spellingShingle | Fabio Aníbal Cristiano Manuel Alberto Pérez Rubén Santiago Nicholls Angela Patricia Guerra Polymorphism in the Plasmodium vivax msp 3: gene in field samples from Tierralta, Colombia Memorias do Instituto Oswaldo Cruz Plasmodium vivax Colombia genetic polymorphism |
| title | Polymorphism in the Plasmodium vivax msp 3: gene in field samples from Tierralta, Colombia |
| title_full | Polymorphism in the Plasmodium vivax msp 3: gene in field samples from Tierralta, Colombia |
| title_fullStr | Polymorphism in the Plasmodium vivax msp 3: gene in field samples from Tierralta, Colombia |
| title_full_unstemmed | Polymorphism in the Plasmodium vivax msp 3: gene in field samples from Tierralta, Colombia |
| title_short | Polymorphism in the Plasmodium vivax msp 3: gene in field samples from Tierralta, Colombia |
| title_sort | polymorphism in the plasmodium vivax msp 3 gene in field samples from tierralta colombia |
| topic | Plasmodium vivax Colombia genetic polymorphism |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762008000500015 |
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