Abnormal neurobehavior in fish early life stages after exposure to cyanobacterial exudates
Cyanobacterial harmful algal blooms (cHABs) pose a risk to exposed aquatic and terrestrial species. Numerous studies have addressed effects of single toxins while much less attention has been devoted to mixtures of cHAB metabolites that are continually released by living cyanobacteria. Neuro-impairm...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-10-01
|
| Series: | Ecotoxicology and Environmental Safety |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651322009599 |
| _version_ | 1811204154446577664 |
|---|---|
| author | Wenwen Cai Hugh J. MacIsaac Runbing Xu Jinlong Zhang Xiaofu Pan Yuanwei Zhang Junxing Yang Brian Dixon Jiaojiao Li Yuanyan Zi Xuexiu Chang |
| author_facet | Wenwen Cai Hugh J. MacIsaac Runbing Xu Jinlong Zhang Xiaofu Pan Yuanwei Zhang Junxing Yang Brian Dixon Jiaojiao Li Yuanyan Zi Xuexiu Chang |
| author_sort | Wenwen Cai |
| collection | DOAJ |
| description | Cyanobacterial harmful algal blooms (cHABs) pose a risk to exposed aquatic and terrestrial species. Numerous studies have addressed effects of single toxins while much less attention has been devoted to mixtures of cHAB metabolites that are continually released by living cyanobacteria. Neuro-impairment associated with cHABs has been reported in fish, though the mechanism remains unclear. Here we exposed embryos of Sinocyclocheilus grahami, an endangered fish, to Microcystis aeruginosa exudates (MaE) to evaluate neurotoxicity and the toxicity mechanism(s). We found that MaE affected embryonic development by increasing malformation and mortality rates and decreasing the fertilization rate. MaE also inhibited fish neurobehavior including touch response, social frequency, swimming distance, and aggravated light-stimulation response. Neurobehavior suppression resulted from a decrease in excitatory neurotransmitters acetylcholine and dopamine, even though receptors increased. MaE also affected gene and protein expression of neurotransmitters, synthetic and/or degrading enzymes, and receptors. Our findings shed light on specific mechanisms by which MaE induces neurotoxicity in early life stages in fish and contributes to improvement of the conservation strategy for this species. |
| first_indexed | 2024-04-12T03:07:37Z |
| format | Article |
| id | doaj.art-c57b24c2e9e64fda89c986f76ecf9b9a |
| institution | Directory Open Access Journal |
| issn | 0147-6513 |
| language | English |
| last_indexed | 2024-04-12T03:07:37Z |
| publishDate | 2022-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj.art-c57b24c2e9e64fda89c986f76ecf9b9a2022-12-22T03:50:28ZengElsevierEcotoxicology and Environmental Safety0147-65132022-10-01245114119Abnormal neurobehavior in fish early life stages after exposure to cyanobacterial exudatesWenwen Cai0Hugh J. MacIsaac1Runbing Xu2Jinlong Zhang3Xiaofu Pan4Yuanwei Zhang5Junxing Yang6Brian Dixon7Jiaojiao Li8Yuanyan Zi9Xuexiu Chang10School of Ecology and Environmental Science, Yunnan University, Kunming 650091, China; Great Lakes Institute for Environmental Research, University of Windsor, Windsor, ON N9B 3P4, CanadaSchool of Ecology and Environmental Science, Yunnan University, Kunming 650091, China; Great Lakes Institute for Environmental Research, University of Windsor, Windsor, ON N9B 3P4, CanadaSchool of Ecology and Environmental Science, Yunnan University, Kunming 650091, ChinaSchool of Ecology and Environmental Science, Yunnan University, Kunming 650091, ChinaYunnan Key Laboratory of Plateau Fish Breeding, Yunnan Engineering Research Center for Plateau-Lake Health and Restoration, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, ChinaYunnan Key Laboratory of Plateau Fish Breeding, Yunnan Engineering Research Center for Plateau-Lake Health and Restoration, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, ChinaYunnan Key Laboratory of Plateau Fish Breeding, Yunnan Engineering Research Center for Plateau-Lake Health and Restoration, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, ChinaDepartment of Biology, University of Waterloo, Waterloo, ON N2L 3G1, CanadaSchool of Ecology and Environmental Science, Yunnan University, Kunming 650091, ChinaSchool of Ecology and Environmental Science, Yunnan University, Kunming 650091, China; Great Lakes Institute for Environmental Research, University of Windsor, Windsor, ON N9B 3P4, CanadaGreat Lakes Institute for Environmental Research, University of Windsor, Windsor, ON N9B 3P4, Canada; College of Agronomy and Life Sciences, Kunming University, Kunming 650214, China; Corresponding author at: College of Agronomy and Life Sciences, Kunming University, Kunming 650214, China.Cyanobacterial harmful algal blooms (cHABs) pose a risk to exposed aquatic and terrestrial species. Numerous studies have addressed effects of single toxins while much less attention has been devoted to mixtures of cHAB metabolites that are continually released by living cyanobacteria. Neuro-impairment associated with cHABs has been reported in fish, though the mechanism remains unclear. Here we exposed embryos of Sinocyclocheilus grahami, an endangered fish, to Microcystis aeruginosa exudates (MaE) to evaluate neurotoxicity and the toxicity mechanism(s). We found that MaE affected embryonic development by increasing malformation and mortality rates and decreasing the fertilization rate. MaE also inhibited fish neurobehavior including touch response, social frequency, swimming distance, and aggravated light-stimulation response. Neurobehavior suppression resulted from a decrease in excitatory neurotransmitters acetylcholine and dopamine, even though receptors increased. MaE also affected gene and protein expression of neurotransmitters, synthetic and/or degrading enzymes, and receptors. Our findings shed light on specific mechanisms by which MaE induces neurotoxicity in early life stages in fish and contributes to improvement of the conservation strategy for this species.http://www.sciencedirect.com/science/article/pii/S0147651322009599Microcystis aeruginosaSinocyclocheilus grahamiEmbryonic developmentNeurotoxicityEndangered speciesCyanobacterial blooms |
| spellingShingle | Wenwen Cai Hugh J. MacIsaac Runbing Xu Jinlong Zhang Xiaofu Pan Yuanwei Zhang Junxing Yang Brian Dixon Jiaojiao Li Yuanyan Zi Xuexiu Chang Abnormal neurobehavior in fish early life stages after exposure to cyanobacterial exudates Ecotoxicology and Environmental Safety Microcystis aeruginosa Sinocyclocheilus grahami Embryonic development Neurotoxicity Endangered species Cyanobacterial blooms |
| title | Abnormal neurobehavior in fish early life stages after exposure to cyanobacterial exudates |
| title_full | Abnormal neurobehavior in fish early life stages after exposure to cyanobacterial exudates |
| title_fullStr | Abnormal neurobehavior in fish early life stages after exposure to cyanobacterial exudates |
| title_full_unstemmed | Abnormal neurobehavior in fish early life stages after exposure to cyanobacterial exudates |
| title_short | Abnormal neurobehavior in fish early life stages after exposure to cyanobacterial exudates |
| title_sort | abnormal neurobehavior in fish early life stages after exposure to cyanobacterial exudates |
| topic | Microcystis aeruginosa Sinocyclocheilus grahami Embryonic development Neurotoxicity Endangered species Cyanobacterial blooms |
| url | http://www.sciencedirect.com/science/article/pii/S0147651322009599 |
| work_keys_str_mv | AT wenwencai abnormalneurobehaviorinfishearlylifestagesafterexposuretocyanobacterialexudates AT hughjmacisaac abnormalneurobehaviorinfishearlylifestagesafterexposuretocyanobacterialexudates AT runbingxu abnormalneurobehaviorinfishearlylifestagesafterexposuretocyanobacterialexudates AT jinlongzhang abnormalneurobehaviorinfishearlylifestagesafterexposuretocyanobacterialexudates AT xiaofupan abnormalneurobehaviorinfishearlylifestagesafterexposuretocyanobacterialexudates AT yuanweizhang abnormalneurobehaviorinfishearlylifestagesafterexposuretocyanobacterialexudates AT junxingyang abnormalneurobehaviorinfishearlylifestagesafterexposuretocyanobacterialexudates AT briandixon abnormalneurobehaviorinfishearlylifestagesafterexposuretocyanobacterialexudates AT jiaojiaoli abnormalneurobehaviorinfishearlylifestagesafterexposuretocyanobacterialexudates AT yuanyanzi abnormalneurobehaviorinfishearlylifestagesafterexposuretocyanobacterialexudates AT xuexiuchang abnormalneurobehaviorinfishearlylifestagesafterexposuretocyanobacterialexudates |