Morphometric Study on the Energy Drink Induced Pancreatitis on Wistar Albino Rats
Objective: To assess the effects of energy drinks on the micrometric measurements of pancreas of Wistar Albino rats. Methods: This experimental study was conducted in the Department of Anatomy, in collaboration with the animal house of Basic Medical Sciences Institute (BMSI), Jinnah postgraduate m...
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| Format: | Article |
| Language: | English |
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Dow University of Health Sciences
2020-12-01
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| Series: | Journal of the Dow University of Health Sciences |
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| Online Access: | https://jduhs.com/index.php/jduhs/article/view/984 |
| _version_ | 1797810665643048960 |
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| author | Fatima Rehman Zia Ul Islam Sama ul Haq Lubna Faisal Sadia Rehman Santosh Kumar |
| author_facet | Fatima Rehman Zia Ul Islam Sama ul Haq Lubna Faisal Sadia Rehman Santosh Kumar |
| author_sort | Fatima Rehman |
| collection | DOAJ |
| description | Objective: To assess the effects of energy drinks on the micrometric measurements of pancreas of Wistar Albino rats.
Methods: This experimental study was conducted in the Department of Anatomy, in collaboration with the animal house of Basic Medical Sciences Institute (BMSI), Jinnah postgraduate medical center (JPMC), Karachi from September to October 2018. Thirty adult male Wistar Albino rats were equally divided into three groups (Group A as control, while groups B and C were administered 7.5ml and 15ml orally for four weeks, respectively. Pancreas was dissected out at the end of study. After tissue processing, slides were made and stained with hematoxylin and eosin stain (H&E) to observe the morphology and cytoarchitecture of the tissue.
Results: The findings of the study revealed that the mean diameter of pancreatic acini and islets were significantly lower in Group B (12.70 +- 1.20), (63.52 +- 8.98) as compared to Group A (26.29 +- 4.37), (123.66 +- 22.08) respectively, p-value <0.05. Diameter of pancreatic acini and islets were significantly less in Group C (10.06 +- 0.89), (44.58 +- 7.66) as compared to both Groups A (26.29 +- 4.37), (123.66 +- 22.08) and Group B (12.70 +- 1.20), (63.52 +- 8.98) respectively, p-value <0.01. The parenchyma of the observed organ was also damaged with loss of normal cytoarchitecture as observed on microscopy.
Conclusion: The results obtained in the current study suggest that consumption of energy drinks seriously damages the regular anatomy of the pancreas of Wistar Albino rats.
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| first_indexed | 2024-03-13T07:11:11Z |
| format | Article |
| id | doaj.art-c57fb4c168ad4027a1fdc6bd8d4e8b0d |
| institution | Directory Open Access Journal |
| issn | 1995-2198 2410-2180 |
| language | English |
| last_indexed | 2024-03-13T07:11:11Z |
| publishDate | 2020-12-01 |
| publisher | Dow University of Health Sciences |
| record_format | Article |
| series | Journal of the Dow University of Health Sciences |
| spelling | doaj.art-c57fb4c168ad4027a1fdc6bd8d4e8b0d2023-06-06T03:12:19ZengDow University of Health SciencesJournal of the Dow University of Health Sciences1995-21982410-21802020-12-0114310.36570/jduhs.2020.3.984Morphometric Study on the Energy Drink Induced Pancreatitis on Wistar Albino RatsFatima Rehman0Zia Ul Islam1Sama ul Haq2Lubna Faisal3Sadia Rehman4Santosh Kumar5Department of Anatomy, Liaquat National Hospital and Medical College Karachi, Pakistan.Department of Anatomy, Liaquat National Hospital and Medical College Karachi, Pakistan.Department of Anatomy, Fazaia Ruth Pfau Medical College, Karachi, PakistanDepartment of Anatomy, Liaquat National Hospital and Medical College Karachi, Pakistan.Department of Biochemistry, Bahria University Medical & Dental College, Karachi, PakistanDepartment of Nephrology, Jinnah Post Graduate Medical Center Karachi, Pakistan.Objective: To assess the effects of energy drinks on the micrometric measurements of pancreas of Wistar Albino rats. Methods: This experimental study was conducted in the Department of Anatomy, in collaboration with the animal house of Basic Medical Sciences Institute (BMSI), Jinnah postgraduate medical center (JPMC), Karachi from September to October 2018. Thirty adult male Wistar Albino rats were equally divided into three groups (Group A as control, while groups B and C were administered 7.5ml and 15ml orally for four weeks, respectively. Pancreas was dissected out at the end of study. After tissue processing, slides were made and stained with hematoxylin and eosin stain (H&E) to observe the morphology and cytoarchitecture of the tissue. Results: The findings of the study revealed that the mean diameter of pancreatic acini and islets were significantly lower in Group B (12.70 +- 1.20), (63.52 +- 8.98) as compared to Group A (26.29 +- 4.37), (123.66 +- 22.08) respectively, p-value <0.05. Diameter of pancreatic acini and islets were significantly less in Group C (10.06 +- 0.89), (44.58 +- 7.66) as compared to both Groups A (26.29 +- 4.37), (123.66 +- 22.08) and Group B (12.70 +- 1.20), (63.52 +- 8.98) respectively, p-value <0.01. The parenchyma of the observed organ was also damaged with loss of normal cytoarchitecture as observed on microscopy. Conclusion: The results obtained in the current study suggest that consumption of energy drinks seriously damages the regular anatomy of the pancreas of Wistar Albino rats. https://jduhs.com/index.php/jduhs/article/view/984AciniDiameterEnergy drinkIslets of Langerhan’sParenchyma |
| spellingShingle | Fatima Rehman Zia Ul Islam Sama ul Haq Lubna Faisal Sadia Rehman Santosh Kumar Morphometric Study on the Energy Drink Induced Pancreatitis on Wistar Albino Rats Journal of the Dow University of Health Sciences Acini Diameter Energy drink Islets of Langerhan’s Parenchyma |
| title | Morphometric Study on the Energy Drink Induced Pancreatitis on Wistar Albino Rats |
| title_full | Morphometric Study on the Energy Drink Induced Pancreatitis on Wistar Albino Rats |
| title_fullStr | Morphometric Study on the Energy Drink Induced Pancreatitis on Wistar Albino Rats |
| title_full_unstemmed | Morphometric Study on the Energy Drink Induced Pancreatitis on Wistar Albino Rats |
| title_short | Morphometric Study on the Energy Drink Induced Pancreatitis on Wistar Albino Rats |
| title_sort | morphometric study on the energy drink induced pancreatitis on wistar albino rats |
| topic | Acini Diameter Energy drink Islets of Langerhan’s Parenchyma |
| url | https://jduhs.com/index.php/jduhs/article/view/984 |
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