The dynamic change of antibody index against Covid-19 is a powerful diagnostic tool for the early phase of the infection and salvage PCR assay errors
Background: Currently, PCR assay is a golden standard for diagnosis of Covid-19. However, it needs nasopharyngeal swabs, expensive instruments and expertise. It even causes PCR errors. Methods: We validated the antibody assay (Roche) in 36 followed patients and 1879 controls (medical staffs). Result...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2021-10-01
|
| Series: | Journal of Microbiology, Immunology and Infection |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1684118221000086 |
| _version_ | 1818896842627219456 |
|---|---|
| author | Masao Omata Yosuke Hirotsu Hiroki Sugiura Makoto Maejima Yuki Nagakubo Kenji Amemiya Miyoko Hayakawa Toshiharu Tsutsui Yumiko Kakizaki Hitoshi Mochizuki Yoshihiro Miyashita |
| author_facet | Masao Omata Yosuke Hirotsu Hiroki Sugiura Makoto Maejima Yuki Nagakubo Kenji Amemiya Miyoko Hayakawa Toshiharu Tsutsui Yumiko Kakizaki Hitoshi Mochizuki Yoshihiro Miyashita |
| author_sort | Masao Omata |
| collection | DOAJ |
| description | Background: Currently, PCR assay is a golden standard for diagnosis of Covid-19. However, it needs nasopharyngeal swabs, expensive instruments and expertise. It even causes PCR errors. Methods: We validated the antibody assay (Roche) in 36 followed patients and 1879 controls (medical staffs). Results: Of 1879 medical staffs, only two (0.11%) were positive by Cut off Index (COI; 1.0) (mean ± SD, 0.094 ± 0.047). Thirty six patients were composed of three groups; Group A,4 from Diamond Princess cruise ship, Group B, 2 infected in Africa, and Group C, 30 infected in Japan. PCR assays were conducted at outside laboratories before and repeated in house after hospitalized. Of 36 at admission, positive antibody was seen in 4/4 from the ship, 0/2 from Africa, and 5/30 from Japan. Two from Africa showed the increase of COI and became positive on days 8 and 13. Thirty Japanese was divided in two groups, e.g., 23 showed dynamic increase of COI up to 84.4 within 3 days while active virus replication present (Group C). In remaining 7 (7/30, 23%) (Group C'), no rise of antibody nor positive in house PCR assays, indicative of false positive results of PCR at the beginning. Conclusion: This antibody testing has a wide dynamic ranges of COI and, thus, could be utilized in the early infection phase. This may also compliment and even help to avoid possible PCR errors. Therefore, this can serve as a powerful diagnostic tool, needed in the frontline of the clinic and hospitals. |
| first_indexed | 2024-12-19T19:06:42Z |
| format | Article |
| id | doaj.art-c58044cae40143e98c5c7e8a3e240724 |
| institution | Directory Open Access Journal |
| issn | 1684-1182 |
| language | English |
| last_indexed | 2024-12-19T19:06:42Z |
| publishDate | 2021-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Microbiology, Immunology and Infection |
| spelling | doaj.art-c58044cae40143e98c5c7e8a3e2407242022-12-21T20:09:26ZengElsevierJournal of Microbiology, Immunology and Infection1684-11822021-10-01545830838The dynamic change of antibody index against Covid-19 is a powerful diagnostic tool for the early phase of the infection and salvage PCR assay errorsMasao Omata0Yosuke Hirotsu1Hiroki Sugiura2Makoto Maejima3Yuki Nagakubo4Kenji Amemiya5Miyoko Hayakawa6Toshiharu Tsutsui7Yumiko Kakizaki8Hitoshi Mochizuki9Yoshihiro Miyashita10Genome Analysis Center, Yamanashi Central Hospital, 1-1-1 Fujimi, Kofu, Yamanashi, Japan; The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, Japan; Corresponding author. University of Tokyo, Yamanashi Central Hospital, Kofu, Japan. Fax: +81 55 253 8011.Genome Analysis Center, Yamanashi Central Hospital, 1-1-1 Fujimi, Kofu, Yamanashi, JapanDivision of Clinical Biochemistry and Immunology, Yamanashi Central Hospital, 1-1-1 Fujimi, Kofu, Yamanashi, JapanDivision of Microbiology in Clinical Laboratory, Yamanashi Central Hospital, 1-1-1 Fujimi, Kofu, Yamanashi, JapanDivision of Microbiology in Clinical Laboratory, Yamanashi Central Hospital, 1-1-1 Fujimi, Kofu, Yamanashi, Japan; Division of Genetics and Clinical Laboratory, Yamanashi Central Hospital, 1-1-1 Fujimi, Kofu, Yamanashi, JapanDivision of Genetics and Clinical Laboratory, Yamanashi Central Hospital, 1-1-1 Fujimi, Kofu, Yamanashi, JapanCentral Clinical Laboratory, Yamanashi Central Hospital, 1-1-1 Fujimi, Kofu, Yamanashi, JapanLung Cancer and Respiratory Disease Center, Yamanashi Central Hospital, 1-1-1 Fujimi, Kofu, Yamanashi, JapanLung Cancer and Respiratory Disease Center, Yamanashi Central Hospital, 1-1-1 Fujimi, Kofu, Yamanashi, JapanGenome Analysis Center, Yamanashi Central Hospital, 1-1-1 Fujimi, Kofu, Yamanashi, Japan; Central Clinical Laboratory, Yamanashi Central Hospital, 1-1-1 Fujimi, Kofu, Yamanashi, Japan; Department of Gastroenterology, Yamanashi Central Hospital, 1-1-1 Fujimi, Kofu, Yamanashi, JapanLung Cancer and Respiratory Disease Center, Yamanashi Central Hospital, 1-1-1 Fujimi, Kofu, Yamanashi, JapanBackground: Currently, PCR assay is a golden standard for diagnosis of Covid-19. However, it needs nasopharyngeal swabs, expensive instruments and expertise. It even causes PCR errors. Methods: We validated the antibody assay (Roche) in 36 followed patients and 1879 controls (medical staffs). Results: Of 1879 medical staffs, only two (0.11%) were positive by Cut off Index (COI; 1.0) (mean ± SD, 0.094 ± 0.047). Thirty six patients were composed of three groups; Group A,4 from Diamond Princess cruise ship, Group B, 2 infected in Africa, and Group C, 30 infected in Japan. PCR assays were conducted at outside laboratories before and repeated in house after hospitalized. Of 36 at admission, positive antibody was seen in 4/4 from the ship, 0/2 from Africa, and 5/30 from Japan. Two from Africa showed the increase of COI and became positive on days 8 and 13. Thirty Japanese was divided in two groups, e.g., 23 showed dynamic increase of COI up to 84.4 within 3 days while active virus replication present (Group C). In remaining 7 (7/30, 23%) (Group C'), no rise of antibody nor positive in house PCR assays, indicative of false positive results of PCR at the beginning. Conclusion: This antibody testing has a wide dynamic ranges of COI and, thus, could be utilized in the early infection phase. This may also compliment and even help to avoid possible PCR errors. Therefore, this can serve as a powerful diagnostic tool, needed in the frontline of the clinic and hospitals.http://www.sciencedirect.com/science/article/pii/S1684118221000086Covid-19Early diagnosisAntibody testPCR assay |
| spellingShingle | Masao Omata Yosuke Hirotsu Hiroki Sugiura Makoto Maejima Yuki Nagakubo Kenji Amemiya Miyoko Hayakawa Toshiharu Tsutsui Yumiko Kakizaki Hitoshi Mochizuki Yoshihiro Miyashita The dynamic change of antibody index against Covid-19 is a powerful diagnostic tool for the early phase of the infection and salvage PCR assay errors Journal of Microbiology, Immunology and Infection Covid-19 Early diagnosis Antibody test PCR assay |
| title | The dynamic change of antibody index against Covid-19 is a powerful diagnostic tool for the early phase of the infection and salvage PCR assay errors |
| title_full | The dynamic change of antibody index against Covid-19 is a powerful diagnostic tool for the early phase of the infection and salvage PCR assay errors |
| title_fullStr | The dynamic change of antibody index against Covid-19 is a powerful diagnostic tool for the early phase of the infection and salvage PCR assay errors |
| title_full_unstemmed | The dynamic change of antibody index against Covid-19 is a powerful diagnostic tool for the early phase of the infection and salvage PCR assay errors |
| title_short | The dynamic change of antibody index against Covid-19 is a powerful diagnostic tool for the early phase of the infection and salvage PCR assay errors |
| title_sort | dynamic change of antibody index against covid 19 is a powerful diagnostic tool for the early phase of the infection and salvage pcr assay errors |
| topic | Covid-19 Early diagnosis Antibody test PCR assay |
| url | http://www.sciencedirect.com/science/article/pii/S1684118221000086 |
| work_keys_str_mv | AT masaoomata thedynamicchangeofantibodyindexagainstcovid19isapowerfuldiagnostictoolfortheearlyphaseoftheinfectionandsalvagepcrassayerrors AT yosukehirotsu thedynamicchangeofantibodyindexagainstcovid19isapowerfuldiagnostictoolfortheearlyphaseoftheinfectionandsalvagepcrassayerrors AT hirokisugiura thedynamicchangeofantibodyindexagainstcovid19isapowerfuldiagnostictoolfortheearlyphaseoftheinfectionandsalvagepcrassayerrors AT makotomaejima thedynamicchangeofantibodyindexagainstcovid19isapowerfuldiagnostictoolfortheearlyphaseoftheinfectionandsalvagepcrassayerrors AT yukinagakubo thedynamicchangeofantibodyindexagainstcovid19isapowerfuldiagnostictoolfortheearlyphaseoftheinfectionandsalvagepcrassayerrors AT kenjiamemiya thedynamicchangeofantibodyindexagainstcovid19isapowerfuldiagnostictoolfortheearlyphaseoftheinfectionandsalvagepcrassayerrors AT miyokohayakawa thedynamicchangeofantibodyindexagainstcovid19isapowerfuldiagnostictoolfortheearlyphaseoftheinfectionandsalvagepcrassayerrors AT toshiharutsutsui thedynamicchangeofantibodyindexagainstcovid19isapowerfuldiagnostictoolfortheearlyphaseoftheinfectionandsalvagepcrassayerrors AT yumikokakizaki thedynamicchangeofantibodyindexagainstcovid19isapowerfuldiagnostictoolfortheearlyphaseoftheinfectionandsalvagepcrassayerrors AT hitoshimochizuki thedynamicchangeofantibodyindexagainstcovid19isapowerfuldiagnostictoolfortheearlyphaseoftheinfectionandsalvagepcrassayerrors AT yoshihiromiyashita thedynamicchangeofantibodyindexagainstcovid19isapowerfuldiagnostictoolfortheearlyphaseoftheinfectionandsalvagepcrassayerrors AT masaoomata dynamicchangeofantibodyindexagainstcovid19isapowerfuldiagnostictoolfortheearlyphaseoftheinfectionandsalvagepcrassayerrors AT yosukehirotsu dynamicchangeofantibodyindexagainstcovid19isapowerfuldiagnostictoolfortheearlyphaseoftheinfectionandsalvagepcrassayerrors AT hirokisugiura dynamicchangeofantibodyindexagainstcovid19isapowerfuldiagnostictoolfortheearlyphaseoftheinfectionandsalvagepcrassayerrors AT makotomaejima dynamicchangeofantibodyindexagainstcovid19isapowerfuldiagnostictoolfortheearlyphaseoftheinfectionandsalvagepcrassayerrors AT yukinagakubo dynamicchangeofantibodyindexagainstcovid19isapowerfuldiagnostictoolfortheearlyphaseoftheinfectionandsalvagepcrassayerrors AT kenjiamemiya dynamicchangeofantibodyindexagainstcovid19isapowerfuldiagnostictoolfortheearlyphaseoftheinfectionandsalvagepcrassayerrors AT miyokohayakawa dynamicchangeofantibodyindexagainstcovid19isapowerfuldiagnostictoolfortheearlyphaseoftheinfectionandsalvagepcrassayerrors AT toshiharutsutsui dynamicchangeofantibodyindexagainstcovid19isapowerfuldiagnostictoolfortheearlyphaseoftheinfectionandsalvagepcrassayerrors AT yumikokakizaki dynamicchangeofantibodyindexagainstcovid19isapowerfuldiagnostictoolfortheearlyphaseoftheinfectionandsalvagepcrassayerrors AT hitoshimochizuki dynamicchangeofantibodyindexagainstcovid19isapowerfuldiagnostictoolfortheearlyphaseoftheinfectionandsalvagepcrassayerrors AT yoshihiromiyashita dynamicchangeofantibodyindexagainstcovid19isapowerfuldiagnostictoolfortheearlyphaseoftheinfectionandsalvagepcrassayerrors |