The HP0256 gene product is involved in motility and cell envelope architecture of <it>Helicobacter pylori</it>
<p>Abstract</p> <p>Background</p> <p><it>Helicobacter pylori </it>is the causative agent for gastritis, and peptic and duodenal ulcers. The bacterium displays 5-6 polar sheathed flagella that are essential for colonisation and persistence in the gastric muco...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2010-04-01
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| Series: | BMC Microbiology |
| Online Access: | http://www.biomedcentral.com/1471-2180/10/106 |
| _version_ | 1811298567575306240 |
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| author | Penn Charles W Moore Stanley A Caly Delphine L Lane Michael C Ryan Kieran A Douillard François P Hinds Jason O'Toole Paul W |
| author_facet | Penn Charles W Moore Stanley A Caly Delphine L Lane Michael C Ryan Kieran A Douillard François P Hinds Jason O'Toole Paul W |
| author_sort | Penn Charles W |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p><it>Helicobacter pylori </it>is the causative agent for gastritis, and peptic and duodenal ulcers. The bacterium displays 5-6 polar sheathed flagella that are essential for colonisation and persistence in the gastric mucosa. The biochemistry and genetics of flagellar biogenesis in <it>H. pylori </it>has not been fully elucidated. Bioinformatics analysis suggested that the gene HP0256, annotated as hypothetical, was a FliJ homologue. In <it>Salmonella</it>, FliJ is a chaperone escort protein for FlgN and FliT, two proteins that themselves display chaperone activity for components of the hook, the rod and the filament.</p> <p>Results</p> <p>Ablation of the HP0256 gene in <it>H. pylori </it>significantly reduced motility. However, flagellin and hook protein synthesis was not affected in the HP0256 mutant. Transmission electron transmission microscopy revealed that the HP0256 mutant cells displayed a normal flagellum configuration, suggesting that HP0256 was not essential for assembly and polar localisation of the flagella in the cell. Interestingly, whole genome microarrays of an HP0256 mutant revealed transcriptional changes in a number of genes associated with the flagellar regulon and the cell envelope, such as outer membrane proteins and adhesins. Consistent with the array data, lack of the HP0256 gene significantly reduced adhesion and the inflammatory response in host cells.</p> <p>Conclusions</p> <p>We conclude that HP0256 is not a functional counterpart of FliJ in <it>H. pylori</it>. However, it is required for full motility and it is involved, possibly indirectly, in expression of outer membrane proteins and adhesins involved in pathogenesis and adhesion.</p> |
| first_indexed | 2024-04-13T06:21:15Z |
| format | Article |
| id | doaj.art-c5841496e3604ebea3b14f0b9cb981e0 |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-04-13T06:21:15Z |
| publishDate | 2010-04-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-c5841496e3604ebea3b14f0b9cb981e02022-12-22T02:58:38ZengBMCBMC Microbiology1471-21802010-04-0110110610.1186/1471-2180-10-106The HP0256 gene product is involved in motility and cell envelope architecture of <it>Helicobacter pylori</it>Penn Charles WMoore Stanley ACaly Delphine LLane Michael CRyan Kieran ADouillard François PHinds JasonO'Toole Paul W<p>Abstract</p> <p>Background</p> <p><it>Helicobacter pylori </it>is the causative agent for gastritis, and peptic and duodenal ulcers. The bacterium displays 5-6 polar sheathed flagella that are essential for colonisation and persistence in the gastric mucosa. The biochemistry and genetics of flagellar biogenesis in <it>H. pylori </it>has not been fully elucidated. Bioinformatics analysis suggested that the gene HP0256, annotated as hypothetical, was a FliJ homologue. In <it>Salmonella</it>, FliJ is a chaperone escort protein for FlgN and FliT, two proteins that themselves display chaperone activity for components of the hook, the rod and the filament.</p> <p>Results</p> <p>Ablation of the HP0256 gene in <it>H. pylori </it>significantly reduced motility. However, flagellin and hook protein synthesis was not affected in the HP0256 mutant. Transmission electron transmission microscopy revealed that the HP0256 mutant cells displayed a normal flagellum configuration, suggesting that HP0256 was not essential for assembly and polar localisation of the flagella in the cell. Interestingly, whole genome microarrays of an HP0256 mutant revealed transcriptional changes in a number of genes associated with the flagellar regulon and the cell envelope, such as outer membrane proteins and adhesins. Consistent with the array data, lack of the HP0256 gene significantly reduced adhesion and the inflammatory response in host cells.</p> <p>Conclusions</p> <p>We conclude that HP0256 is not a functional counterpart of FliJ in <it>H. pylori</it>. However, it is required for full motility and it is involved, possibly indirectly, in expression of outer membrane proteins and adhesins involved in pathogenesis and adhesion.</p>http://www.biomedcentral.com/1471-2180/10/106 |
| spellingShingle | Penn Charles W Moore Stanley A Caly Delphine L Lane Michael C Ryan Kieran A Douillard François P Hinds Jason O'Toole Paul W The HP0256 gene product is involved in motility and cell envelope architecture of <it>Helicobacter pylori</it> BMC Microbiology |
| title | The HP0256 gene product is involved in motility and cell envelope architecture of <it>Helicobacter pylori</it> |
| title_full | The HP0256 gene product is involved in motility and cell envelope architecture of <it>Helicobacter pylori</it> |
| title_fullStr | The HP0256 gene product is involved in motility and cell envelope architecture of <it>Helicobacter pylori</it> |
| title_full_unstemmed | The HP0256 gene product is involved in motility and cell envelope architecture of <it>Helicobacter pylori</it> |
| title_short | The HP0256 gene product is involved in motility and cell envelope architecture of <it>Helicobacter pylori</it> |
| title_sort | hp0256 gene product is involved in motility and cell envelope architecture of it helicobacter pylori it |
| url | http://www.biomedcentral.com/1471-2180/10/106 |
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